Western Blotting Products & Reviews

4X Protein Sample Loading Buffer is optimized for use as a loading buffer for protein gel electrophoresis. This orange loading buffer is recommended for use with Odyssey® Imaging Systems as it does not fluoresce in the 700 channel.

Chameleon Vue Pre-stained Protein Ladder offers multi-colored, pre-stained proteins for molecular weight estimation

Chameleon Duo Pre-stained Protein Ladder provides multi-colored, pre-stained bands for visual inspection and two-color near-infrared detection. It includes 11 protein bands from 8 to 260 kDa.

IRDye Labeling Kits come in 3 forms and allow you to label your own peptides, antibodies, or proteins.

IRDye 800CW secondary antibodies are the antibodies of choice for a wide variety of applications in the 800 channel of Odyssey ® Imaging Systems.

Odyssey Loading Indicators (OLI) provide a simple, convenient method to evaluate the consistency of sample loading volume across gel lanes, as well as the uniformity of Western blot transfer.

The Odyssey Pen is suitable for writing on membranes to be scanned on any of the Odyssey Imagers.

Western Blot Storage Bags are ideal for long-term storage of near-infrared fluorescence Western blot membranes.

The Odyssey XF is the perfect entry point for labs transitioning from qualitative chemiluminescent Western blotting to more robust quantitative data analysis. Drawing from more than 30 years of imaging expertise, the Odyssey XF offers unmatched dynamic range with the click of a mouse. In addition to two NIR lasers, it has LED and CCD capabilities, providing multi-functional versatility for greater value.

CellTag 520 Stain is a visible fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications.

IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes.

A high-throughput fluorescence-based assay for protein stability screening with higher tolerance for detergents and other protein additives compared to commonly used Thermal Shift dyes.

Quench lipofuscin autofluorescence in PBS, with minimal background.

Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification; with your choice of next-generation fluorescent CF® dyes or biotin.

Label your antibody with one of our superior CF ®  Dyes in 15 minutes without a purification step. Labeling tolerates many common buffer components including BSA and ascites.

Buffer system optimized for fluorescence-based western provides superior specificity, sensitivity, and background suppression.

The AttoPhos® AP Fluorescent Substrate System provides a highly sensitive fluorescent alkaline phosphatase substrate.   The system includes AttoPhos® Substrate, AttoPhos® Buffer and Calibration Solution. AttoPhos® Substrate (2′-[2-benzothiazoyl]-6′-hydroxybenzothiazole phosphate [BBTP]) is cleaved by alkaline phosphatase to produce inorganic phosphate (Pi) and the alcohol, 2′-[2-benzothiazoyl]-6´-hydroxybenzothiazole (BB…

BioIVT has the expertise in the processes required for ethical acquisition of biospecimens that cover a wide range of disease states and the protocols needed to produce reliable data. With over 425 IRB-approved clinical sites, BioIVT provides clinical specimens for nearly all disease states.

A concentrated Ponceau S protein staining solution designed for rapid and reversible visualization of proteins on nitrocellulose and PVDF membranes. Ideal for efficiently assessing protein transfer quality in western blot workflows while allowing flexible preparation of working solutions.

IRIS9 Prestained Protein Ladder is a ready-to-use prestained protein molecular weight marker composed of 9 recombinant proteins ranging from 15 to 180 kDa. It is designed for real-time monitoring of protein separation during SDS-PAGE and for evaluating protein transfer efficiency in Western blot workflows.

A ready-to-use, reversible Ponceau S protein staining solution for rapid visualization of proteins on nitrocellulose and PVDF membranes. Designed to quickly assess protein transfer efficiency after western blotting without interfering with downstream immunodetection.

OneStep Blocker, Catalog No. BS001-B500ML, 500 ml; suffices for ≥25 mini-gel membranes, stable for 1 year at 25 °C. Protein-free Western blot reagent, enables one-step blocking/antibody hybridization, enhances HRP/AP signals; suitable for Western Blot/ELISA/ECL, compatible with PVDF/NC Membrane, HRP/AP.

eLITE Xe MultiSpectral Light Source is a fast and efficient process for enabling researchers to achieve simultaneous detection. The eLITE MultiSpectral Light Source utilizes high-intensity xenon or halogen lighting in conjunction with fiber optic guides to supply powerful, directed illumination of live plants, animals, and other fluorescent stained samples in Analytik Jena's BioImaging Systems.   

Recombinant Human Antibody (D25) is capable of binding to RSV, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-RSV mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-RSV mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.

Recombinant monoclonal antibody to CD274. This antibody is a human IgG1 monoclonal antibody that targets and binds to PD-L1, a protein that is often overexpressed on the surface of cancer cells and immune cells in the tumor microenvironment.