Western Blotting Products & Reviews

When performing two-color detection, loading controls and normalization are essential for reliable, precise comparison of protein levels. LICORbio offers primary antibodies for normalization that can be used for two-color normalization when performing multiplex Western blots or for cell-based assay normalization.

WesternSure ECL Stripping Buffer provides a robust solution for stripping primary and secondary antibodies from Western blot membranes while maintaining target antigen integrity for efficient reprobing.

NewBlot IR Stripping Buffer is ideal for stripping and reprobing nitrocellulose and PVDF membranes detected with IRDye ® secondary antibodies. It may also be suitable for other dye-conjugated secondary antibodies.

CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications. The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers.

Chameleon®  Pre-Stained Protein Ladders offer easy visualization of gel migration and protein size, and can be used to orient your gel or membrane quickly. These versatile protein markers offer multi-colored, pre-stained proteins for both visual inspection and two-color near-infrared detection. Choose from either single-channel or two-channel detection.

The WesternSure Protein Ladder is the only pre-stained, multi-colored protein ladder that provides chemiluminescent signal for film and digital detection. It will fit with your current protocol without optimization and does not rely on strep-tagged HRP or secondary antibody binding. The WesternSure protein ladder works with a variety of substrates and provides a vibrant color scheme that allows you to quickly orient your membr…

4X Protein Sample Loading Buffer is optimized for use as a loading buffer for protein gel electrophoresis. This orange loading buffer is recommended for use with Odyssey® Imaging Systems as it does not fluoresce in the 700 channel.

Chameleon Vue Pre-stained Protein Ladder offers multi-colored, pre-stained proteins for molecular weight estimation

Chameleon Duo Pre-stained Protein Ladder provides multi-colored, pre-stained bands for visual inspection and two-color near-infrared detection. It includes 11 protein bands from 8 to 260 kDa.

IRDye Labeling Kits come in 3 forms and allow you to label your own peptides, antibodies, or proteins.

IRDye 800CW secondary antibodies are the antibodies of choice for a wide variety of applications in the 800 channel of Odyssey ® Imaging Systems.

Odyssey Loading Indicators (OLI) provide a simple, convenient method to evaluate the consistency of sample loading volume across gel lanes, as well as the uniformity of Western blot transfer.

The Odyssey Pen is suitable for writing on membranes to be scanned on any of the Odyssey Imagers.

Western Blot Storage Bags are ideal for long-term storage of near-infrared fluorescence Western blot membranes.

The Odyssey XF is the perfect entry point for labs transitioning from qualitative chemiluminescent Western blotting to more robust quantitative data analysis. Drawing from more than 30 years of imaging expertise, the Odyssey XF offers unmatched dynamic range with the click of a mouse. In addition to two NIR lasers, it has LED and CCD capabilities, providing multi-functional versatility for greater value.

CellTag 520 Stain is a visible fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications.

IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes.

V10-SDB 10 x 10cm and V20-SDB 20 x 20cm semi-dry blotters are ideal for fast transfer of proteins and nucleic acids without the need for costly accessories such as gel cassettes and tanks. Corrosion-free platinum-coated titanium and stainless steel plate electrodes, corresponding to the anode and cathode, maximise the active area of transfer so that full electrical contact is made between the gel and the membrane, allowing tr…

Fast sample-to-membrane hybridisation guaranteed for immediate screening Available in alphanumeric 24, 48 and 96 sample throughputs, Scie-Plas's dot and slot blot microfiltration manifolds provide an easy and reproducible means to hybridise proteins and nucleic acids in solution onto membranes. These manifolds, which are machined from high-density acrylic, have precision-lapped mating surfaces that ensure uniform filter/membra…

Syngene GeneTools - Advanced 1D gel analysis Syngene GeneTools is an advanced 1D gel and 2D spot blot analysis software for use with any gel documentation system.  The Syngene GeneTools highly automated software can rapidly analyse a gel from loading an image to output of results in a matter of seconds.  With an extensive and flexible array of options you can handle a range of media including gels, plates, films and blots,…

Anti-cdc42 is a mouse monoclonal IgG1 that recognizes cdc42 from human, mouse, rat and dog

The ChIPAb+ CREB, antibody/primer set includes the CREB, antibody, a negative control antibody (purified rabbit IgG), and qPCR primers which amplify a 64 bp region flanking the CRE of the human cFos gene. The CREB and negative control cultured supernatant are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of CREB associated chromatin.

Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. Sp1 antibody and the negative control antibody (rabbit normal IgG) can be used to demonstrate that the Sp1 antibody is functionally validated in the precipitation of Sp1 associated chromatin. The qPCR…

Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. LEF1 antibody and the negative control antibody (mouse normal IgG) can be used to demonstrate that the LEF1 antibody is functionally validated in the precipitation of LEF1 associated chromatin.The qPC…

The ChIPAb+ HDAC1 set includes the HDAC1 antibody, the negative control antibody supernatant (mouse IgG), and qPCR primers flanking an Sp1 binding site in human p21 (WAF1/CIP1/CDKN1A) promoter, amplifying a 105 base pair PCR product. The HDAC1 and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of HDAC1 associated chromatin.