Next-Generation Sequencing Products & Reviews

Kit for determining the nucleotide sequence (in extra-long reads, up to 1kb) of an immobilized and clonally amplified DNA library prepared using the GS FLX+ System methods for the preparation of DNA libraries and amplified by the emPCR amplification process, using the appropriate GS FLX Titanium series kit(s). For use in combination with the GS FLX Titanium PicoTiterPlate Kit 70 × 75 and the GS FLX+ Instrument. Contents The GS…

The Roche Genome Sequencer FLX System, powered by 454 Sequencing, enables ground-breaking discoveries in de novo sequencing, resequencing of whole genomes and target DNA regions, metagenomics, and RNA analysis. Featuring a unique combination of long reads, exceptional accuracy, and ultra-high throughput, the Genome Sequencer FLX System delivers the most comprehensive result at a low total cost, giving it the best overall value…

VAHTS ® Universal Plus DNA Library Prep Kit for Illumina is a DNA library construction kit developed for the Illumina ® high-throughput sequencing platform.

Stop gambling on cycle numbers. The icon96 uses iconPCR™ technology with AutoNorm™ software to provide real-time fluorescence feedback in each of 96 independent wells, preventing under-amplification dropouts and over-amplification artifacts—delivering balanced, sequencing-ready libraries without manual quantification or normalization.

The icon16™ brings AutoNorm™ adaptive amplification to lower-throughput NGS workflows, with up to 16 samples at a time. Designed for labs that refuse to compromise on data quality, it delivers per-well real-time fluorescence monitoring and automatic library normalization in a compact, benchtop footprint.

Unlocking the potential of genomics with miniaturized, automated liquid handling 

Rapidly design your NGS panels for tumor genomic profiling to fit your specific requirements, including incorporation of new and emerging biomarkers. Leverage the curated content of the SureSelect Cancer CGP and Tumor-Specific catalog DNA panels to add or subtract gene content in the SureDesign web portal.

SureSelect Cancer Tumor-Specific assays offer genomic profiling for solid tumors from lung, colon, pancreas, kidney, or bladder tissue, to detect single nucleotide variants (SNVs), copy number variants (CNVs), insertions/deletions (indels), and translocations (TLs). These small cancer panels enable affordable tumor genomic profiling.

Agilent SureSelect DNA panels are predefined designs which focus on targeted gene sets for specific applications. Agilent offers two human panels, the SureSelect Human Kinome panel and SureSelect X-Chromosome panel. The SureSelect Human DNA Kinome panel targets a comprehensive set of kinases and kinase related genes for enrichment, targeting over 500 kinases and 612 genes. The SureSelect X-Chromosome panel targets all of the g…

Agilent's GeneSpring provides powerful, accessible statistical tools for intuitive data analysis and visualization. 

SureCall is a research desktop application combining both novel and widely accepted opensource algorithms for end-to-end NGS data analysis from alignment to categorization and annotation of mutations, supporting Agilent Target Enrichment applications. 

Agilent SureSelect All Exon kits are the most widely used target enrichment solution for exome sequencing. SureSelect Human All Exon V5 (targeting coding regions only) and V5+UTRs (targeting coding regions plus UTRs) have been updated to provide the greatest sequencing efficiency and enable samples ready for sequencing the next day. Greater coverage with less sequencing for lower overall costs – up to 60% less sequencing than…

Agilent Solutions for Automated NGS Sample Prep provide: Flexibility to automate virtually any NGS reagent Performance to dramatically increase throughput and walk-away time Optimized SureSelectXT protocols to rapidly automate your NGS Sample Prep Agilent NGS Option A and Option B Both Deliver Higher Throughput with Less Hands-On Time Automating your NGS sample preparation with either Agilent NGS Option A or Option B…

The SureSelect Strand-Specific RNA Library Preparation kit prepares Poly(A) enriched libraries for whole-transcriptome sequencing. Sequence a wide range of RNA to detect gene expression changes and alternative splicing. The kit contains mRNA sequencing library preparation and enrichment materials.

The HaloPlex Cancer Research Panel enables fast, simple, and efficient analysis of genomic regions of interest for cancer research for a large number of sample types, including FFPE. HaloPlex Cancer is uniquely suited to high performance with cancer research samples, which are commonly preserved as formalin fixed and paraffin embedded (FFPE). This FFPE process often results in highly fragmented DNA, resulting in insufficient…

Agilent’s GeneSpring NGS software suite includes data analysis workflows for Methyl-Seq, RNA-Seq, DNA-Seq, Chip-Seq and Small RNA-Seq data. GeneSpring NGS provides SureSelect customers with an easy to use QC, visualization and reporting tool for Methyl-Seq, RNA-Seq and DNA-Seq applications. GeneSpring NGS also allows the detection of transcriptomic changes like splice variants and gene fusions, or identify structural variation…

HaloPlex technology provides outstanding performance, streamlined workflow, and low sample input requirements for next generation sequencing of human exomes. The HaloPlex Exome has been optimized to provide comprehensive coverage of the coding regions of the human genome with minimal hands on time and requires only 200ng of input DNA. The Haloplex Exome performance and workflow is ideal for clinical research: • Outstanding cov…

Agilent Haloplex custom kits offer the flexibility to create custom panels for any genomic regions of interest. A unique and efficient library prep-free workflow enables complete target enrichment in less than 6 hours from just 200 ng of input DNA.

Agilent HaloPlex panels are predefined designs which focus on targeted gene sets for specific applications. Agilent offers two catalog HaloPlex panels, the Cancer Research Panel and the Cardiomyopathy panel.The HaloPlex Cardiomyopathy (catalog) is a next generation sequencing target enrichment panel designed specifically for inherited forms of cardiomyopathy. Following a careful review of cardiomyopathy publications as well as…

The SureSelect Focused Exome is a highly targeted design that enables analysis of only disease-associated targets, enabling superior coverage of disease-associated regions even on benchtop sequencers. This design provides 20 or more reads for 95% of targets at 1.5Gb (100x) of sequencing and 98% of targets with 3Gb (200x) of sequencing.

The Magnis system provides a complete workflow for NGS library preparation that is fully automated and delivers reproducible results. Built on proven Agilent SureSelect XT HS2 chemistry, start from total RNA with integrated cDNA conversion or unfragmented DNA with onboard enzymatic fragmentation for increased workflow efficiency.

Unlock the full potential of molecular diagnostics on a single platform

SureSelect Cancer CGP assay offers comprehensive genomic profiling for solid tumors. This pan-cancer NGS panel enables detection of somatic variants, including single nucleotide variants (SNVs), copy number variants (CNVs), insertions/deletions (indels), translocations (TLs), gene fusions, and the immuno-oncology biomarkers TMB (tumor mutational burden) and MSI (microsatellite instability).

Designed for smaller studies, the SureSelect DNA Capture Arrays complement Agilent's in-solution SureSelect Target Enrichment System, which is designed for medium to large-scale NGS studies of tens through thousands of samples, including automated high-throughput workflows.

Agilent’s market leading SureSelect platform provides a complete portfolio of catalog and custom products, providing the flexibility you need from discovery to follow-up studies. Agilent’s custom solutions offer the only available method for target enrichment of RNA, allowing you to target your regions of interest using our free web portal SureDesign. Custom solutions are available for DNA and RNA target enrichment. • The only…