PCR and Thermal Cycling Products & Reviews

UltraScript® 2.0 cDNA Synthesis Kit is a 2-tube kit containing all the components required for fast, reliable and unbiased cDNA synthesis of the most challenging RNA sample types.

UltraScript® 2.0 Reverse Transcriptase is a highly thermostable reverse transcriptase designed for cDNA synthesis of the most challenging RNA sample types. This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55 °C, giving improved specificity, higher cDNA yields and more full length cDNA product.

qPCRBIO SyGreen® Blue Mix uses a non-reactive blue dye for easy sample visualisation during reaction setup, allowing fast, highly sensitive and reproducible real-time PCR with more precision and accuracy than ever. This easy-to-see mix is particularly useful in reducing errors when loading small volume reactions or using white plates.

VeriFi® Mix is a versatile and robust high fidelity 2x ready-mix engineered for all PCR applications where greater sequence accuracy is required. Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets. 

VeriFi® Hot Start Mix is a versatile and robust 2x proofreading ready-mix with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

VeriFi® Hot Start Mix Red is a versatile and robust 2x proofreading ready-mix with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates. The mix includes a convenient red dye for direct gel loading.

VeriFi® Hot Start Polymerase is a versatile and robust proofreading enzyme with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

qPCRBIO Probe Blue Mix is a universal probe kit that includes a non-reactive blue dye for easy sample visualisation and enhanced pipetting accuracy. Whether your application is for a singleplex or multiplex expression study or for genotyping and allelic discrimination analyses, qPCRBIO Probe Blue Mix is the robust choice for all your probe-based real-time PCR needs.

PCRBIO HiFi Polymerase is our original high-fidelity enzyme, derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. Several proprietary point mutations allow for significantly improved performance when compared with its native form. Together with advanced buffer chemistry this enzyme brings robust performance to the world of high fidelity PCR.

PCRBIO Ultra Polymerase has been engineered for the amplification of extremely difficult templates. Proprietary modifications that enhance processivity together with advanced buffer chemistry and hot start technology deliver outstanding performance whether your template is GC or AT-rich, low in abundance or contains PCR inhibitors.

PCRBIO Ultra Mix is a convenient 2x ready mix that has been engineered for the amplification of extremely difficult templates. Proprietary modifications that enhance processivity together with advanced buffer chemistry and hot start technology deliver outstanding performance whether your template is GC or AT-rich, low in abundance or contains PCR inhibitors.

PCRBIO Ultra Mix is a convenient 2x ready mix that has been engineered for the amplification of extremely difficult templates, with a red dye included for direct gel loading.

UltraScript® cDNA Synthesis Kit is an easy-to-use 2-tube system specifically developed to generate cDNA for use in real-time PCR. The kit provides unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations.

PCRBIO 1-Step Go RT-PCR Kit is a convenient, easy-to-use kit for fast and efficient cDNA synthesis and PCR in a single tube. The advanced buffer system, reverse transcriptase and hot start polymerase give highly specific and ultra-sensitive 1-step RT-PCR from any RNA template including mRNA, total RNA and viral RNA sequences.

qPCRBIO Probe 1-Step Virus Detect is designed for ultra-sensitive detection of viral RNA by 1-step RT-qPCR using sequence-specific probes.

From collecting samples to reading results, the Q-POC™ SARS-CoV-2 assay has been developed considering patient and user experience at every step of the COVID-19 testing process. The test delivers rapid PCR results in approximately 30 minutes at the Point of Need, on the accurate and portable Q-POC™ platform.

Diagnosing, treating and managing clinically similar respiratory infections can be challenging.  The Q-POC™ SARS-CoV-2, Flu A/B & RSV multiplex assay provides rapid detection and differentiation of three leading respiratory diseases in just ~35 minutes. Running on the “one sample, one test” portable Q-POC™ platform, the test provides results you can trust at the Point of Need.

CFX Opus Dx Real-Time PCR Systems combine the flexibility you need in your workflow and outstanding performance to deliver reliable results that your clinical laboratory demands.

A PCR detection system with 96-well block and 2-color detection plus FRET. Includes CFX Duet base unit and cables.

Highly discriminatative master mix for mismatches at the 3’-terminus of primers in PCR.  HiDi® Taq 2x PCR Master Mix is a ready-to-use aptamer-based fast-start formulated master mix specially optimized for assays in which high discrimination is required. With this unique mix less than 10 copies of a mutation can be detected in a background of >10^4 wild-type copies.  

Volcano3G ® RT-PCR Probe 2x Master Mix contains an engineered, highly thermostable DNA Taq polymerase with reverse transcriptase activity that enables amplification of RNA target sequences with quick and easy PCR protocols, even including "zero-step" amplification.  

The 5x concentration of PlexTaq® 5x qPCR Multiplex Master Mix makes it optimal for multiplexing applications, leaving more free volume for primers and probes, and samples. The combination of aptamer-based fast-start Taq DNA polymerase, optimized reaction buffer, and ultrapure dNTPs, ensures consistent results, rapid set-up time, and a lower risk of pipetting errors.   

 MedixMDx qRT-PCR Lyo ready mix is ready to be lyophilized to produce stable reagents at room temperature. Upon addition of target specific primers/probes and reverse transcriptase (RTase Lyo) to the mastermix, the mixture can be lyophilized directly, without the need to add additional excipients.  

MedixMDx HiPlex qRT-Probe Mix is formulated as a 4x mix, which enables extensive multiplexing and addition of larger volumes of RNA templates to reactions. It includes an efficient thermostable reverse transcriptase with RNase inhibitor to prevent degradation of RNA templates by RNases.  

Lyo-Ready Probe 1-Step Kit is a lyophilisable (freeze-dryable) RT-qPCR kit designed for ultra-sensitive detection of RNA sequences.