VeriFi® Mix
VeriFi® Mix is a versatile and robust high fidelity 2x ready-mix engineered for all PCR applications where greater sequence accuracy is required. Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets.
A ready-mix with a high fidelity enzyme
Greater sensitivity and specificity
Versatility across amplicon lengths
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VeriFi® Mix includes VeriFi® Polymerase which is derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. The enzyme is engineered with proprietary mutations that significantly increase processivity, resulting in shorter extension times (10-30 s/kb), higher yields and the ability to amplify longer and more difficult targets, including eukaryotic genomic templates in excess of 17.5 kb.
The high accuracy and enhanced 3’-5’ exonuclease activity of VeriFi® Polymerase result in fidelity that is approximately 100 times higher than Taq DNA polymerase. The mix is ideally suited to applications where greater accuracy is required, such as cloning, site-directed mutagenesis and sequencing. PCR products generated with this range of products are blunt ended.
This 2x mix contains all reaction components apart from primers and template.
Features
- High temperature cycling – up to 100 °C denaturation to better separate GC-rich templates
- Increased PCR success rates with complex genomic templates (17.5 kb and over)
- High yields under standard and fast PCR conditions (10-30 s/kb)
- Efficient and specific amplification from challenging templates including GC and AT-rich sequences
- 100x higher fidelity than Taq DNA polymerase
- Generates blunt-end PCR products
Gain better quality data from metagenomic analysis
Traditional microbiology methods enable detection only of a subset of microbes in a sample (only those organisms that can be cultured under laboratory conditions). Metagenomics (the use of NGS, next-generation sequencing, on complex microbial samples) overcomes this limitation by directly extracting and sequencing DNA or RNA from environmental samples, providing a snapshot of the genetic content of the entire microbial community.
See how you can gain better quality data for more reliable conclusions, enhanced comparative analyses with improved statistical power, and greater cost efficiency with the VeriFi™ Library Amplification Mix.
Pioneers in PCR drive next generation technology for NHS and beyond
Meet Liz Alexianu and James Cooper from PCR Biosystems, a company working on the frontier of PCR technology development. Find out how this company is working with customers to deliver high performance PCR solutions that meet the needs of the scientists and clinicians working with them. Plus, learn out how these solutions are having a real world impact in institutions such as the NHS.
This video was filmed at the 2023 ADLM Annual Scientific Meeting and Clinical Lab Expo
