VeriFi® Hot Start Mix Red
VeriFi® Hot Start Mix Red is a versatile and robust 2x proofreading ready-mix with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates. The mix includes a convenient red dye for direct gel loading.
A ready-mix with red-dye for precise PCR
Long range PCR up to 17.5 kb
AptaLock hot start technology
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VeriFi® Hot Start Mix Red is as a convenient 2x ready mix containing all reaction components apart from primers and template. The mix also contains a red dye suitable for direct loading and tracking during agarose gel electrophoresis.
VeriFi® Hot Start Mix Red is powered by VeriFi® Hot Start Polymerase, a single enzyme derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. Proprietary mutations improve DNA binding and increase processivity when compared to its native form, resulting in shorter extension times, higher yields and the ability to amplify longer and more difficult targets. VeriFi® Hot Start Polymerase is able to amplify eukaryotic genomic templates in excess of 17.5 kb, and longer for simpler DNA templates.
PCRBIO’s innovative AptaLock™ technology uses a proprietary aptamer-like molecule that reversibly inhibits activity below 40 °C, preventing primer dimer formation and non-specific amplification to maximise the sensitivity and specificity of your PCR. This feature makes VeriFi® Hot Start Mix Red highly suitable for multiplexing and enables reactions to be set up at room temperature. The reaction mix is stable both before and after the PCR run for up to 24 hours, meaning greater ease of use for high throughput assays.
The enhanced accuracy of VeriFi® Hot Start Polymerase gives extremely low error rates and fidelity that is approximately 100 times higher than Taq DNA polymerase. The enzyme is ideal for applications where superior accuracy is required, such as cloning, site-directed mutagenesis and sequencing.
Features
- AptaLock™ hot start technology for maximised sensitivity and specificity
- Greater success with long and/or GC or AT-rich templates (17.5 kb and over)
- High temperature cycling – up to 100 °C denaturation to better separate GC-rich sequences
- 100x higher fidelity than Taq DNA polymerase
- Room temperature setup
- Reaction mix stability for up to 24 hours both before and after PCR run
- Generates blunt-end PCR products
Pioneers in PCR drive next generation technology for NHS and beyond
Meet Liz Alexianu and James Cooper from PCR Biosystems, a company working on the frontier of PCR technology development. Find out how this company is working with customers to deliver high performance PCR solutions that meet the needs of the scientists and clinicians working with them. Plus, learn out how these solutions are having a real world impact in institutions such as the NHS.
This video was filmed at the 2023 ADLM Annual Scientific Meeting and Clinical Lab Expo
