Genomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.
The CpG WIZ® E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify under the same conditions. Control genomic DNA samples (methylated and…
The CpG WIZ® Prader-Willi/Angelman Amplification Kit is used for determining the methylation status of this region by methylation -specific PCR (MSP). The kit contains primers targeted to the CpG island on the SNRPN gene. PCR parameters have been identified such that both primer sets in the kit amplify template under the same conditions. Normal control genomic DNA, which will generate a product with both primer sets, is also i…
The CpGenome™ DNA Modification Kit is used to create methylation-specific sequence alterations in any DNA sample. Using chemical treatment, the kit converts all unmethylated cytosine residues to uracil. Methylated cytosine residues are unaltered in this process. Therefore, the chemically-treated DNA sequence will differ only if it was originally unmethylated in vivo. After sequence conversion, methylation specific PCR is emplo…
CpGenome™ Universal Methylated DNA is enzymatically methylated human male genomic DNA. This product is intended for use with the CpGenome™ DNA Modification Kit (S7820) or with amplification primers. It can be used as a methylation-positive control for gene methylation studies.
CpGenome™ Universal Unmethylated DNA Set provides 2 separate genomic DNA controls for gene methylation studies. This product is intended for use with the CpGenome™ DNA Modification Kit (S7820) and can be subsequently used as an unmethylated DNA control for gene methylation studies.
The CpGenome™ Fast DNA Modification Kit contains the reagents for the initial bisulfite modification of the DNA required for both methodologies.
The CpGenome™ DNA Modification Kit (Cat. No. S7820) contains the reagents necessary to perform the initial bisulfite reactions, while the CpG WIZ® BRCA1 Amplification Kit contains the reagents required for the PCR amplification reactions.
ChIP-Seq facilitates genome-wide profiling of the interactions of transcription factors, modified histones, and other chromatin associated proteins. By combining chromatin immunoprecipitation with next generation sequencing, laboratories can generate high resolution data on protein-DNA interactions across the genome. For laboratories interested in gaining a deeper understanding of epigenetic mechanisms of gene regulation, ChIP…
Superior Bead Recovery and Low Levels of False Positives Ensure Assay Reproducibility. Smooth well wall provides efficient bead recovery, ensuring reproducible results lot after lot High performance membrane does not trap microspheres in the membrane matrix In serological assays, Supor® membrane effectively removes IgG complexes, thus reducing nonspecific reactivity of the microspheres and reducing false positives New well des…
Robust Chemical Resistance Provides Compatibility with Harsh Organics. Chemically resistant materials provide a stable platform to process samples in organic solvents Inert materials of construction ensure complete recovery of samples and low non-specific binding New well design results in faster, more uniform filtration rates across the plate and reduces hold-up volume Innovative outlet tip geometry provides direct flow of sa…
The AcroPrep™ Advance filter plates for lysate clearance effectively remove cellular debris quickly and allow for the use of additional wash steps to maximize sample recovery. Filtration can be accomplished by either vacuum manifold or centrifugation, ultimately maximizing your choice in protocols. The purification of biomolecules (DNA, RNA and proteins) from prokaryotic and eukaryotic cell lines can be problematic. The AcroP…
ColorKits are designed to facilitate the measurement of the activity of phosphatases, ATPases and other Pi-generating enzynes in microplate assays. Each Kit contains a phosphorylated substrate for a particular class of enzyme, together with a carefully optimised PiColorLock detection reagent, Pi standard, 2x96-well plates and a selection of buffer concentrates for optimisation of assay conditions with the enzyme of interest.…
The LightSwitch promoter reporter GoClone collection includes transfection-ready luciferase reporter constructs utilizing the novel RenSP luciferase technology for over 17,000 human promoters and highly optimized LightSwitch reagents. Measure the functional response of promoters to stimulus in nearly any cell-based system. Plus, advances in gene assay reagent and transfection technology eliminates the need for co-transfection…
An arrayed or non-arrayed BAC library is the preferred choice for downstream applications such as hybridization screening; full-genome sequencing; BAC end sequencing or physical mapping projects. Amount of clones received is based on a guaranteed coverage and 120Kb average insert size. HMW DNA isolation and QC by HMW DNA enzymatic digestion are included. Deliverables for ARRAYED BAC library: Clones will be arrayed and amplifie…
A pooled BAC library is an invaluable tool for PCR-screening. It allows for the easy retrieval of BAC clones of interest. This type of library is especially useful when genome size is large and creating an arrayed library is cost-prohibitive. We typically construct a minimum 5X BAC library and BAC clones are then pooled and distributed in 96-well plates. Each well will contain about 400-500 original BAC clones. HMW DNA isolati…
Bio S&T's high-quality RNA can be used directly for NxGen RNA sequencing as well as other downstream applications that require optimal-quality RNA such as quantitative real-time RT-PCR, microRNA cloning and amplification and Northern blotting.
The PowerPlex® Y23 System is a 23-loci, 5-color Y-STR multiplex designed for genotyping forensic casework samples, database samples and paternity samples. The kit contains: all 12 loci in the current PowerPlex® Y System, the additional 5 loci found in AmpFlSTR® Y-filer®, plus 6 new loci. PowerPlex® Y23 System Benefits: Reveal more Y-STR loci in excess female DNA Cut your amplification time roughly in ha…
GeneJuice® Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity. This transfection reagent is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, and complex formation with DEAE-dextran. Whereas many available transfection reagents…
Pellet Paint® NF Co-Precipitant* is a non-fluorescent dye-labeled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye™ Terminators (PE Corp.) during the alcohol precipitation of cycle sequencing reaction products. Cycle sequencing reactions can be precipitated rapidly with centrifugation times of 10 minutes. The easily visualized carrier provides a simple confirmation that precipitation has o…
Powerful transfection reagent, based on an innovative biochemical method. Next generation of outstanding transfection reagent, highly efficient, universal and multipurpose.
For all nucleic acids and all transfection conditions.The universal and powerful PolyMag formulation allows achieving very high transfection efficiency. It is composed by magnetic nanoparticles coated with cationic molecules. Exclusive and optimized PolyMag reagent has been successfully tested with:- All nucleic acids types: naked DNA, RNA, siRNA, ODN, etc…- Cell lines, primary and hard to transfect cells- Adherent and suspens…
CombiMag improves your transfection reagent efficiencyCombiMag reagent has been designed to be employed in association with all transfection reagents complexed with any types of nucleic acids. All cell types, even hard-to-transfect and primary cells, can be transfected using CombiMag associated to any working reagent, even if its original efficiency is low.CombiMag has been associated with many existing transfection reagents i…
FlyFectin ™ is a specifically designed and very efficient transfection reagent for insect cells. Innovative and unique, FlyFectin ™ is: • Highly efficient with excellent reproducibility• Multipurpose (various types of nucleic acids)• Ideal for recombinant protein production with Baculovirus expression system• Simple, Ready-to-use and Rapid• Compatible with and without serum-containing culture media• Non toxic• Economi…
