Gene Expression and Molecular Cloning Products & Reviews

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The control kit includes validated positive control targeting RAB1A and non-targeting control for assay set-up and a UCOE KRAB-dCas9 for consistent effector expression across a wide variety of cell lines. The kit is ideal for setting up the screen assay and optimization before using the CRISPRi library pools.

The 10X CRISPRi Feature Barcode Optimization kit is ideally suited for setting up the screen assay and if desired capture sequence optimization before using the Custom 10x Compatible CRISPRi library pools.

This pooled library targets the human kinome, a collection of approximately 700 genes commonly associated with development and disease, with an average of nearly 9 unique knockout CRISPR clones per kinase gene. The lentiviral format allows for stable integration, selection, and enrichment of transduced cells

Utilize the power of CRISPR Whole Genome Lentiviral Pools to knockout and screen every gene in the human genome. The Sigma Whole Human Genome Lentiviral CRISPR Pool is provided as 200 ul in 8 x 25 ul aliquots at a minimum titer of 5x108 viral particles/ml via p24

Accomplish highly effective transfection of CRISPR/Cas9 DNA, RNA, Cas9-gRNA RNP (ribonucleoprotein) complexes and siRNA to knock-out, modify or knock-down genes of interest in a wide variety of cell types.

Recombinant Cas9-D10A Nickase protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments.

The Sigma lenti CRISPR Non-Targeting Control is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. This vector is useful as a negative control in experiments using Sigma CRISPR lentiviral clones.

Human CRISPRi Kinase Phosphatase Drug Targets Kit contains 2 sub-pools of top 5 gRNAs per gene as well as a separate sub-pool of 5 supplemental gRNAs per gene for increased sensitivity and includes non-targeting controls built in.

The Lenti CRISPR Non-Target Control#1 (LV04 vector) is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments).

The Sigma lenti CRISPR Non-Targeting Control is a lentiviral system, which includes a gRNA sequence that does not target known human, mouse and rat genes. The non-targeting control lentivirus is useful as a negative control in experiments using Sigma CRISPR lentiviral clones.

Pooled CRISPR screening with 10x genomics compatibility for discovery at single cell resolution

The Gator ® High Sensitivity (HS) AAV9 Kit enable high sensitivity quantification of AAV9 in crude and purified samples. The HS AAV9 Kit includes: HS AAV9 probes (96 probes/tray) HS AAV9 Detection Solution AAV Amplification Solution AAV Substrate Solution AAV Substrate Diluent Q Buffer ** Contains temperature sensitive components. Must be shipped overnight. ** *Not compatible with Gator ® AAV9 probe (Part No. 160021)

This product selection tool identifies your best solution for high transfection performance

Designed for exceptional DNA delivery efficiency into hard-to-transfect, primary, and cancer cell lines.

The most trusted delivery brand now offering cutting-edge lipid nanoparticle (LNP) solutions with proven safety, delivery efficiency and specificity for your nucleic acid drug delivery needs.

The first viral feed designed specifically for HEK293 cells

The Gator ® AAV Ratio Kit enables empty vs full determination of AAV serotypes 1-10 and chimeric serotypes in crude and purified samples. The AAV Ratio Kit includes: AAVX probes (96 probes/tray) SSB probes (96 probes/tray) Lysis Buffer DNA Detection Solution AAV Ratio Substrate Q Buffer SS DNA Standard Lysis tubes ** Contains temperature sensitive components. Must be shipped overnight. **

Complete ligation reactions in just 15 minutes, efficient cloning at low concentrations of DNA, increased productivity and efficiency.

A simple 7-minute sample preparation procedure is the first part of a complete workflow provided by the TaqMan® Gene Expression Cells-to-CT™ Kit. Remove the guesswork of assembling separate RNA purification, RT, and real-time PCR kits, and achieve unsurpassed performance and sensitivity in gene expression analysis, including detection of siRNA-induced mRNA knockdown. - Complete solution—Optimized workflow includes cell lysis r…

X-tremeGENE siRNA Transfection Reagent efficiently delivers short interfering RNA (siRNA) into many commonly used cell types including HeLa, NIH 3T3, HEK-293, CHO-K1, and COS-7, and several hard-to-transfect cell lines such as HT29, a human adenocarcinoma cell line. When used for cotransfection-based gene knockdown, X-tremeGENE siRNA Transfection Reagent enables significant protein expression and effective knockdown levels due…

Upgrade from the Rapid DNA Ligation Kit and choose the new Rapid DNA Dephos & Ligation Kit for fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments. The convenient kit enables ligation of DNA in 5 minutes and dephosphorylation in 10 minutes. This kit contains the new recombinant rAPid Alkaline Phosphatase, the tool of choice for dephosphorylation of 5' phosphates from nucleic acids. This kit…

The Transcriptor First Strand cDNA Synthesis Kit is designed to reverse transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources for the following applications: Studying gene expression levels, via two-step RT-PCR, using qualitative RT-PCR or quantitative RT-PCR on the LightCycler® Carousel-Based System, the LightCycler® 480 System, or other real-time instruments. Generating cDN…

The Transcriptor High Fidelity cDNA Synthesis Kit is designed to reverse transcribe RNA with an increased fidelity compared to other reverse transcriptases. The kit features Transcriptor High Fidelity Reverse Transcriptase, a blend of a recombinant reverse transcriptase and a proofreading mediating enzyme optimized for two-step RT-PCR, making this the product of choice for the following applications: Cloning genes of inter…

Dual-Light® luminescent reporter gene assay is designed for the rapid and sensitive detection of firefly luciferase and b-galactosidase in a single extract aliquot for gene expression assays. Detection of firefly luciferase and b-galactosidase in the same sample simplifies normalization of transfection efficiency Wide dynamic range of b-galactosidase and luciferase assays enables accurate measurement of both enzymes from…