CRISPRi for Transcriptional Interference
A direct and efficient set of tools to study a gene’s function by transcriptional interference in its native context
The system requires two components to operate: a guide RNA and a catalytically deactivated Cas9 (dCas9)
CRISPR without the cut: a novel CRISPR interference system enabling rapid functional gene characterization
This webinar will highlight the Dharmacon™ CRISPRmod CRISPRi system as detailed in a peer-reviewed article in The CRISPR Journal.
Learn first-hand from the inventor about its proprietary effector specifically developed for use with synthetic single guide RNAs (sgRNAs). The system enables functional gene characterization in arrayed format experiments making it a powerful, orthogonal method to siRNA and CRISPR knockout screening.
Key learning objectives
- Learn about a novel CRISPRi effector, dCas9-SALL1-SDS3, that mediates more potent repression when used with synthetic sgRNAs than previously described effectors such as dCas9-KRAB and dCas9-KRAB-MeCP2.
- Discover how dCas9-SALL1-SDS3 interacts with key members of the histone deacetylase and Swi-independent 3 complexes to mediate rapid, potent target gene repression.
- Learn about the development of in vitro-transcribed dCas9-SALL1-SDS3 mRNA for delivery into clinically relevant cell types such as human induced pluripotent stem cells and primary T cells.
- Explore the functional gene characterization of DNA damage host factors using dCas9-SALL1-SDS3 with synthetic sgRNAs, demonstrating the ability of the system to be used in arrayed-format screening.
Who should attend?
- Academics and industry professionals interested in functional genomics, specifically gene editing and gene modulation (CRISPR-Cas9 and RNAi technologies)
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
