Products & Reviews

The ONE-Glo™ + Tox Assay combines luciferase assay chemistry with a cell viability marker to better understand reporter gene expression in the context of cell health. The assay uses a two-step, addition-only process to make these measurements in a single well of a plate, negating the need to run parallel assays. The first part of the assay is a nonlytic fluorescence assay (CellTiter-Fluor™ Cell Viability Assay) that measure…

The Strep-tag® / Strep-Tactin® purification system allows the isolation of above 95% pure and functional proteins after one chromatographical step. The Strep-tag system is based on the highly selective and easily controllable interaction between the Strep-tag II peptide and the specially engineered streptavidin called Strep-Tactin. The binding affinity of Strep-tag II to Strep-Tactin is nearly 100 times higher than to Stre…

High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System(a,b,c) is designed for this purpose by providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media without prior sample…

TaqMan®  Gene Signature Arrays enable analysis of hundreds of TaqMan®  Gene Expression Assays on a 384-well micro fluidic card with minimal effort. TaqMan Gene Signature Arrays are readily available and delivered 2–4 days after ordering. They are packaged conveniently with two or four arrays per pack, making them cost effective, and just the right size for any experiment. Choose from pathway-specific pre-defined arrays such…

The Applied Biosystems 7300 Real-Time PCR System gives you advanced, multicolor real-time PCR capabilities with exceptional budget sensitivity. Run time of less than 2 hours Four-color detection enables you to perform gene expression analysis, pathogen quantitation, SNP genotyping, and plus/minus assays with internal positive controls Study gene expression, detect and quantitate pathogens, perform allelic discrimination…

Millipore’s The FlowCellect™ Human Embryonic Stem Cell Surface Marker Characterization kit is designed to facilitate an easier and quicker way to track surface marker expression of TRA-1-60, SSEA4 and SSEA1. The kit will also help to determine the percentage of undifferentiated human embryonic stem cells in culture by determining the percentage of cells that express both TRA-1-60 and SSEA4 and not SSEA1. This quick test will a…

Easy-to-use and flexible gene expression master mix for real-time PCR Features of PowerTrack SYBR Green Master Mixes include: Two-color tracking dye system indicates where pipetting has occurred Broad primer Tm and primer concentration compatibility allows flexibility in qPCR reaction setup with minimal optimization Superior specificity and tight reproducibility in Ct values over a broad dynamic range improve data…

Making MS operation easierfrom tuning to final report      The all new Agilent MassHunter Workstation software provides intuitive, yet powerful, instrument control, data acquisition, qualitative and quantitative data analysis, and reporting for Agilent time-of-flight (TOF), quadrupole time-of-flight (Q-TOF), ICP-MS, and triple quadrupole systems. Designed from the ground up to make MS analyses easierfrom tuning to final report…

Combined with the Nadia Instrument, the scRNA-Seq Reagent Kit generates PCR amplified cDNA libraries for high throughput gene expression profiling of heterogenous cell populations in just 20 minutes. The kit allows researchers to achieve highly reproducible single cell data sets – with ultra-efficient gene capture rates – at low sequencing costs. The kit contains all the reagents and consumables required for the generation…

Rapidly isolate and purify total RNA from a variety of biological sources. • Fast and easy processing using rapid spin-column format • Isolate all sizes of RNA including large RNA and small RNA species • No phenol or chloroform extractions • Isolate and detect RNA from as little as a single animal cell • Purify total RNA from a variety of biological sources such as cultured animal cells, tissue samples, blood, bacteria, yeast…

Chemicon's membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of FPR1 and its ligands. The membrane preparations exhibit a Kd of 0.39 nM for [125I]-WKYMVm. With 5 μg/well FPR1 Membrane Prep and 0.5 nM [125I]-WKYMVm, a greater than 20-fold signal-to-background is…

Chemicon's V1A membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of V1A interactions with its ligands. The membrane preparations exhibit a Kd of 55 pM for [125I]-vasopressin antagonist ([125I]-phenylacetyl-D-Tyr(Me)-Phe-Gln-Asn-Arg -Pro-Arg-Tyr-NH2). With 10 µg/well V1A Membr…

The FlowCellect™ Human NK Cell Characterization Kit includes three directly conjugated antibodies, Anti-CD3-APC, Anti-CD56-PE, and Anti-CD16 FITC. This three color kit is designed to characterize NK within mixed cell populations. The antibodies provided have been carefully titrated to ensure the ability to accurately measure the expression of all markers simultaneously. Sufficient reagents are provided to perform 25 three-colo…

High efficiency electroporation using any instrumentIngenio® Electroporation Kits and Solution provide a universal, high efficiency, low toxicity solution for electroporation of DNA or siRNA into hard to transfect cell types. Electroporation using Ingenio Electroporation Kits and Solution affords increased gene expression in several different cell types with minimal toxicity.Ingenio® Electroporation Solution Features:•High eff…

High efficiency electroporation using any instrumentElectroporation using Ingenio Electroporation Kits and Solution affords increased gene expression in several different cell types with minimal toxicity. Ingenio Kits and Solution are compatible with multiple conventional electroporation instruments including Lonza Amaxa® Nucleofector®, Bio-Rad® Gene Pulser and Harvard-BTX® electroporators.Ingenio® Cuvettes Features:•High effi…

A cell-permeable (thienothienyl)amino-acetamide compound that displays anti-inflammatory properties. Acts as a potent, reversible, ATP-competitive, and highly selective inhibitor of IKK-2 (IC50 ~3-12 µM for IKK-2 homodimer, IKK-1/IKK-2 heterodimer, and IKK-2). Its specificity has been confirmed using a panel of 31 other kinases, including IKK isoforms IKK-1, IKK-i, and TBK-1 (IC50 > 200 µM). Shown to specifically block NF-kB-d…

The human KCNA3 gene encodes the pore-forming subunit of a voltage-gated potassium channel. Kv1.3 channels expressed in lymphocytes are therapeutic targets in autoimmune diseases. Synonyms: hKv1.3 Host cell: CHO-K1, division-arrested Gene name: KCNA3 Mycoplasma status: Negative (MycoAlert Kit) Packaging: Three vials of Cryopreserved irradiated cells, 2x106 cells/vial (6x106 cells total) Growth media: Ham’s F12; 10% FBS Growth…

The human KCNQ4 gene encodes the pore-forming subunit of a voltage-gated potassium channel. KCNQ4 channels expressed in CNS neurons are therapeutic targets in epilepsy. Synonyms: Kv7.4 Host cell: CHO-K1, division-arrested Gene name: KCNQ4 Mycoplasma status: Negative (MycoAlert Kit) Packaging: Three vials of Cryopreserved irradiated cells, 2x106 cells/vial (6x106 cells total) Growth media: Ham’s F12; 10% FBS Growth characteris…

The SCN4A gene encodes the pore-forming subunit of the voltage-gated, skeletal muscle Na+ channel. Nav1.4 is a therapeutic target for treatment of myotonia. Synonyms: Skeletal muscle sodium channel, SkM1 Host cell: CHO-K1, division-arrested Gene name: SCN4A Mycoplasma status: Negative (MycoAlert Kit) Packaging: Three vials of Cryopreserved irradiated cells, 2x106 cells/vial (6x106 cells total) Growth media: Ham’s F12; 10% FBS…

The human Nav1.5 gene encodes the pore-forming subunit of the voltage-gated, cardiac Na+ channel. Nav1.5 is an antiarrhythmic therapeutic target. Synonyms: cardiac sodium channel Host cell: CHO-K1 Gene name: SCN5A Mycoplasma status: Negative (MycoAlert Kit) Packaging: Three vials of Cyropreserved irradiated cells, 2 x 106 cells/vial (6x106 cells total) Growth media: Ham’s F12; 10% FBS Growth characteristics: cells remain viab…

The SCN8A gene encodes the pore-forming subunit of a voltage-gated Na+ channel. Neuronal Nav1.6 channels are therapeutic targets in pain treatment. Synonyms: NaCh6, PN4 Host cell: CHO-K1, division-arrested Gene name: SCN8A Mycoplasma status: Negative (MycoAlert Kit) Packaging: Three vials of Cryopreserved irradiated cells, 2x106 cells/vial (6x106 cells total) Growth media: Ham’s F12; 10% FBS Growth characteristics: cells rema…

Product Overview: The MILLIPLEX® Human Stem Cell Pluripotency Magnetic Bead Panel 1 (Cat. No. 48-617MAG) is a premixed panel to be used for the simultaneous quantification of the following 4 analytes in cell lysates: c-Myc, Nanog, Oct 3/4, and Sox2. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay. Backgro…

Mouse embryonic stem cells (mESCs) are unique cells in that they can be maintained in vitro as pluripotent cells and can be induced to differentiate into many cell types by manipulating culture conditions. mESCs have been used as a model to help define differentiation and signaling pathways in human embryonic stem cells (hESCs), since hESCs are more arduous to work on. Therefore, much attention has been focused on understandin…

High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with the TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix and others. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template quantities. The new High Capacity RNA-to-cDNA formulation reduces reverse transcription time by…

Agilent Human microRNA Microarrays generate complete microRNA profiles in order to provide broad insight into human expression and regulation. Probe design, coupled with a unique labeling method, works in concert to provide the optimal sensitivity and specificity that your work demands.  Content regularly updated from the Sanger miRBase 16.0  Human and human viral miRNAs represented  Optimal sensitivity and specificity th…