Zyppy™ Plasmid Miniprep Kit by Zymo Research

"In our lab we use many plasmids constructs and we perform purification daily. This kit is amazing and safe us time and money without drop quality."

"I am a high school AP Biology teacher. I have only so much time with my young scientists everyday. The Zyppy Plasmid Miniprep kit gave me an extra day every time I used it. On average we do three or four minipreps every year (per class per year). That equates to about three days for more instruction and more downstream applications for plasmid biology, whether it be additional transformation or restriction analysis. The protocol is very streamlined and easy to understand. My 16 and 17 year old students (and some as young as 15) have no problem completing the protocol and getting reproducible results. I've received nothing but spectacular customer service and I as far as value is concerned, I buy this time and time again."

"This kit is very easy to use and time-saving. More importantly, it's relatively cheap compare to other vendors."

"Great quality results with easy protocol. Good value and good customer service!"

"Effective and easy to use, recommended for faster extraction of high quality DNA."

"This product is time efficient and very easy to use."

"The Zyppy™ Plasmid Miniprep kit modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, then purify using our Fast-Spin column technology. Additionally, the innovative colored buffers included in the kit permit error-free visualization identification of complete bacterial cell lysis and neutralization. Our Zyppy™ Plasmid Miniprep Kit is the fastest and simplest method available to efficiently separate plasmid DNA from E. coli. It is a powerful and innovative product."

"I have used Qiagen products for more than 20 years. I think that Qiagen is the number one company in terms of quality control. I had a chance to use Zymo columns, and I realized that Zymo is completely compatible with Qiagen. I am really happy with the quality and ease of use. After extraction of DNA quantity is above 100 ng/ul, which is a remarkable outcome. I suggest you use Zymo systems in your lab."

"The kit produces excellent quality and quantity plasmid DNA (even with low copy). Very easy to handle like any other brand. We use it mainly for sequencing. The sequencing results always come out excellent. Never had any problem. Used it for the last 12 years. The best part of it: It is 2 times cheaper than the other brands and still it offers the best quality outcome. I highly recommend for all labs to use it."

"It is a handy miniprep kit with a big accentuation on the speed of processing samples. To me, it has two advantageous: - no need to centrifuge cell pellets as you add lysis buffer directly into the media; - there is something in the neutralization buffer that helps to stick all of the debris together. One disadvantage: as opposed to the Qiagen kit it is sensitive to the amount of cells you use."

"Overall, this is one of the more efficient and reliable mini preps I have used. I routinely obtain high quality DNA, good enough for sequencing as well as transfections of immortalized cell lines. My final concentration is approximately 250 ng/µl."

"This product gives me good yield. I mostly use it to validate cloning constructs."

"The plasmid purification kit is very rapid, easy to use. Also, the plasmid quality and yields are high. Moreover, this kit is like 50-100 dollars cheaper than what we used before."

"The plasmid isolation kit from Zymo is cost effective, easy to use and quick. The reproducibility and reliability of the kit is awesome. I recommend using the kit as it is equally efficient to many other manufacturers, and is cheaper too."

"Easy and fast protocol, I was able to get good plasmids concentrations with high purity."

"The kit is incredibly easy to use, and faster than most kits on the market. The Zymo sales team has always been incredibly friendly and helpful. The value for money is excellent as the kit is quite affordable."

"This kit offers great value for money, and gives consistently pure and high quality DNA for each prep. There is little variation between preps, and the DNA is immediately usable for other transformations in the lab. It is also very easy to use, with clear instructions, so it takes a very short time to prep many plasmids."

"I did not purchase this miniprep kit, rather I received it as a sample. I really could not be happier with this kit. Because of it's "Pellet-Free" protocol, it saves me so much time that I could easily get purified plasmids within a 45 minute high school period. This really opens up the possibilities and flexibility for my students and allows them to work closer to their own pace. With traditional miniprep kits, I have to have all 8-10 lab groups be on the same page during the protocol. With Zyppy's fast procedure and reduced spin times, my kids can have freedom to get ahead or take their time to gain a better grasp on the science going on in that tube/column. Ease of use: simple: straightforward protocol After-sales care: had an senior account executive reach out to me the next day to ensure proper address and information. Value: Time is money during a high school lesson. Because I can have my kids get a prep done within ten minutes so they can perform their transformations/restriction digests within a period, I would happily pay the $0.83-$1.30/prep. "

"This kit is very easy and straightforward to use. It is one of the cheapest in the market as well. "

"Very efficient and easy to use kit! Would highly recommend!"

"This plasmid miniprep kit works great for our lab, we always get great results using this prep kit. These kits always come in on time as well, we have never had any problems with the shipping or storage of this product. I have used these specific kits several times, high yield of results, and very consistent results. We will continue to use and choose these plasmid miniprep kits over others because of the great results, ease of storing and usage, and the value of the product. The Zyppy Plasmid Miniprep Kit is a valuable and non-replicable product for our laboratory."

"It has high productivity and high quality. It is very easy to use and cheap. We like it very much!"

"We heavily use Zyppy minipreps for our routine cloning work. When purifying plasmid DNA for sanger sequencing or gel electrophoresis, it gives consistently good yields and simplifies / speeds up our workflow."

"I love the quality/price ratio. These work great for lab members, and they are cheap enough that I don't mind letting undergraduates practice with them."

"Zyppy plasmid kit is really easy to use and quick, value for money is superb, great reproducibility."

"This kit is easy to use and competitive with pricing. Instructions are easy to follow. We get good yields for all plasmids we use with the kit."

"Higly efficient, reproducible, less hands on time and economic."

"My entire experience with Zymo has been positive. Their products are the best value, are reliable, and reproducible. I have set up several labs over my career and use Zymo products in each of them."

"Zyppy is easy to use! And I like that I can choose from 600 µL culture or from more volume to spin-down. We get 800 preps - no worry about running out soon."

"We have been purchasing this product for over 5 years now and have to say the consistency between lots if outstanding. I highly recommend this Zyppy plasmid miniprep kit."

"This is my go to miniprep kit. Incredibly affordable, great results every time, and candy in the box. I've set up three research labs and Zymo products are one of the easiest supplier choices I make."

"Zyppy plasmid mini kit delivers a quick and easy way to isolate plasmid DNA of good quality that is compatible with routine molecular biology application. After using for over 10 years, I can't live without it and have stopped using plasmid mini prep kits from any other supplier. I have not contacted the after sale care team about this product, because it has not given me any problem! highly recommended!"

"Zymo kits have been the most consistent of any brand we've tried. They're also very good value. We like the lack of pelleting cells in the Zyppy kit and proceeding directly to lysis of culture. This saves time and to our surprise, the yield wasn't substantially lower."

"Easy to use with clear instructions. Quick to perform and food quality plasmids for downstream applications."

"I achieved reproducible results and the kit is quick for getting plasmid DNA for cloning and downstream applications."

"Great results for the price. It is really fast and easy to perform plasmid DNA extraction."

"This kit is quick and simple, and gives good plasmid yields every time. Other cheap kits have extra steps, extra columns, or the column material flakes out and contaminates the preps. The Zymo kit works as well as the expensive kits."

"Extremely easy to use! Color change for neutralization is helpful. No problems with debris not centrifuging down as with some other kits. High yields and consistent results."

"I use this product every semester, with 48 sophomore/junior undergraduate students. The students are all able to successfully isolate their individual engineered plasmids, most with high concentrations. I personally have color coded the items in the kit and the instructions associated to help make the instructions more transparent and visible for my novice users to follow."

"This is by far the easiest to use and best value miniprep kit I have used. It produces great quality plasmid DNA."

"I started using Zyppy Plasmid kit from a free sample and I am hooked. It is so fast and easy to use! I have used the ZymoPure miniprep kit as well. Both work well, though Zyppy is faster. I recommend this kit because of its speed. The quality of the DNA plasmids that I recover is also good."

"Have used this product for years. Very easy to use, coloured buffers help to visualize neutralisation, high yield, good reproducibility, plasmids can be used for in vitro studies and for cloning as well, and it works so fast (this parameter is very important for me)! Almost all steps require just 15 seconds of centrifugation – usually, I spin down 4ml of culture and add 500ml of mQ to get 600ml final volume to start plasmid purification."

"The product offers simple direction and ease of use, so we are able to give it to the entire lab to use without a problem"

"The DNA concentration was not high, your price is good, but we can't buy large kits because the shelf life of the kit is very short. They always expire so quickly we can't keep them longer."

"This product provides the fastest way to isolate DNA plasmids from E. Coli. It is reliable, easy to use and the purified plasmids are endotoxin-free and ready for transfection!"

"It is easy to use, and its quality is beyond expectation! We use plasmid DNA for the injection into C. elegans to generate transgenic lines without any further purification."

"The kit is easy to use and provides plenty of plasmid DNA to use in down-stream work. It is cheaper than it’s competitors in the market."

"The Zyppy MiniPrep kit is an efficient spin based protocol for the production of plasmid DNA for most routine DNA applications, we have excellent results in both preparative approaches - subcloning, PCR as well as screening. Our favorite kit to date."

"Very good product for isolation of genomic, plasmid DNA from bacteria. I achieved high quality yield and also reproducible results. I used the above mentioned product only in overseas labs (Israel). Easy to use and handle. Minimum number of solutions for better results."

"Very easy to use. Plasmid quality is consistent, although not the best yield and concentration. Other, more expensive kits give higher quality DNA, but for everyday screening, this kit is value for money."

"So far, this kit has been perfect. I have been able to easily make my minipreps, consistently."

"I carried out a comparison against 2 other major brands of plasmid mini prep kit, purifying plasmid from 1ml of pelleted bacteria. The Zyppy kit was the quickest and cheapest per sample (at the time, I haven't compared prices since) of the three. In terms of yield, it also had the highest recovery of our plasmid, which is much larger than average. The kit is simple to use - my only warning being to make sure you add ethanol to the wash buffer when you receive the kit to prevent students using the concentrated solution by mistake. I have no experience of the after sales care."

"The plasmid miniprep kit is very easy to use. On occasions when I have had difficulties and my students obtained poor results, I called tech support and spoke to Michelle and she was able to troubleshoot the problem for us. It's really great to know that whenever I have a problem, I can call tech support and obtain the help that I need."

"I have used many different kits to do minipreps and this one is the easiest and quickest I have used so far, and it is also cheap."

"Much better than Qiagen columns, with higher yield and a shorter protocol!"

"Zymo products have helped us stay on budget, while providing exceptional products."

"This kit is easy to use and reliable. I run a primarily undergraduate laboratory and they rarely have any problems. We have recommended this kit to others at our university and they also have been pleased."

"The product is easy to use and provides very low level endotoxin plasmid DNA. Its also fast - I can complete 20 samples in under 20 minutes!"

"Haven't needed customer support because the kit works, and works well. Very straight forward and simple protocol. Plasmid is ready in a timely fashion. Have not had any problems with the kit ever."

"Fast and easy to use with colored buffers to show when the lysis and neutralisation steps are complete. High yield and the price is very good."

"Good value, we love this product. Reproducible results."

"Even though yield is little bit low, quality of plasmid prep is good."

"High quality DNA concentration. Higher yields when buffer or water is warm. Easy to use."

"This product worked great everytime with clear directions. The only reason for a 3 star after sales care score was because I didn't need any after sales instruction. I will continue to use this kit for restriction digest screening of clones."

"I appreciate the easy use of the kit and the purity of plasmid DNA after preping out of E. coli cells. Even students can use the kit without anything else than the provided manual and get very good results. I think it would be even a bit better, if there were small symbols or something like that on the coloumns to identify them in the centrifuge without having to write on the very small labelling areas. The numbers given there are very small and not so easy to read (actually I'm not even sure, if they are meant for identification or if they are just remains from the production process)."

"I have performed hundreds of mini preps. I can say the following: very easy and intuitive handling, reproducible results in terms of quality and concentration acceptable price and benefit."

"The product was very easy for my undergraduate students to use and understand. Consistently high-quality samples were obtained."

"Easy application, fool-proof for beginners. Great cost and time!"

"It is very easy and fast to use. It gives great results."

"Very fast; no isopropanol precipitation step. Easy to use; few steps and colored buffers to check if each step was run successfully. Great DNA yield and quality."

"We grow a larger overnight culture, then pellet the cells and resuspend in 600 uL to prepare as directed. This does an excellent job of creating high concentration of pure plasmid that reliably works for us in transformation as well as mutagenesis."

"The Zyppy Plasmid Miniprep kit was the first kit that I used that has a color indicator added to show when lysis has been fully neutralized. Because of this feature, the quality and yield of the DNA is better than what I experienced in earlier kits from at least 4 other brands. Some steps have been made more convenient, e.g. there is no need to empty the collector tube between the 2 wash steps. These little things make the entire process just that little faster. I don't like that you have to centrifuge the spin columns without a lid. A tip for resuspending the cells: add only 300 uL first, rash the tubes over the top of a tube rack and then add another 300 uL and the 100 uL lysis buffer. The method doesn't work as well with the 600 uL at once."

"The kit can provide fastest way to get plasmid DNA. Also it is easy to use with highly reproducible results. The instruction is quite simple and user-friendly. The price of the kit is very competitive with other similar kits."

"The Zymo Miniprep kit is extremely easy to use, takes less time than most other kits and gives great results!"

"I would give this product 5-star rating. This kit requires less starting material, yield is great in less time and the quality is fantastic. I never get spark when I perform electroporation. Wonderful products."

"Zyppy Plasmid Miniprep Kit has removed the cumbersome step of pelleting the bacterial culture before the lysis step. Surprisingly enough the yield is good enough for all the downstream applications that one might need the purified plasmid for. It has made life easier!"

"That kit is good, high yield and purity is good, I am satisfied with it."

"The Miniprep kit is very easy to use. The isolated plasmid is very pure and can be used for all subsequent steps."

"Easy to use, quick and reliable plasmid mini-prep kits. And in fact we use Zymo Midi and maxi prep kits as well as the pcr clean kits and RNA clean kits"

"My undergraduate students use this miniprep kit to isolate all the plasmids we routinely use in the lab. It is undergrad-proof, with excellent and quality yields!"

"Excellent product that produces high quality plasmid preps and is easy to use. However, in Canada, it is not competitive with several other products (BioBasic, FroggaBio) based on price. Cheaper than Qiagen last time I checked, and about the same as NEB Monarch."

"Ease of Use- I rated this as mainly on my own wet lab experience using Zymo and Qiagen kits and also teaching to undergraduates. Yields from DH5 alpha E coli so far have been in acceptable ranges (100-300 ng/ul , 260/280 ratios near 1.7,1.8). Some optimizations and changes can be made like using water instead of the final elution buffer (for convenience). The kit is easy to show undergraduates and they can perform the mini preps within a week reliably. The color changes are consistent and comparable to other kits in ease. After-Sales Care- I rated this mainly on shipping and ordering. We receive shipments intact and on time when we order from the Zymo site. No shipping issues so far. Value for Money- I rate this mainly on my ordering experience in the lab. The price is comparable to other kits."

"This prep is super fast, and gives a seriously large amount of transfection-quality DNA from a small culture volume. If I put all the bugs from a 3ml overnight through this kit I've gotten up to 35µg of DNA in a 10 minute prep. I basically don't need any other prep for routine transfections."

"Fastest product in the market. Quality of the DNA is superior."

"I got the "Zyppy™ Pasmid Miniprep Kit" to try it out. I must say that Zymo did not fail me, once again! The plasmids that I have extracted were pure and in record time. I know that the price for the kit is very competitive and even more economically friendly. The buffers have normal life span and are kept at room temperature and some are kept at 4 degrees. Neutralization buffer already has RNase inside so you don’t even have to make that post treatment afterwards."

"This kit is fast, simple and spot-on. We have not had a dud yet that wasn't due to UE (undergraduate error). As a matter of fact, we purchase the kit in the large (400 column) size on a regular basis. The buffers that come with the kit are ample and have a long shelf life. Zymo also helpfully sells the columns separately, so you can get the most value for your money and use all the elements so that nothing goes to waste. On the other hand, you can also buy the buffers separately, so if something mysteriously happens to one of the buffers (as in ending up all over the floor), you can rest easy that you can purchase just the spilled item and not have to buy an entire kit for one component. I've had great results with Zymo technical support and they don't try to upsell you on something that doesn't fit your needs. Oh, yes, and the Miniprep Plasmid Kit is inexpensive (really inexpensive!)."

"Fast, without the hassle of taking multiple Eppendorf tube in and out of microcentrifuge for spinning. The quality of final plasmid DNA is as good as Qiagen QIAquick Plasmid Miniprep Kit."

"These are the fastest, easiest, and most cost effective plasmid prep kits we've tested. Importantly they require the least amount of peripheral equipment, e.g. centrifuges/tubes, vacuum pumps, etc."

"Easy to use, everything is clear. Always get great DNA yield. Fast protocol leading to fast results. Consistent results. "

"This is one of the best products in the category! Best value for money, easy to use, reproducible yield and high quality DNA. Our sequencing issues were solved by using these kits."

"The Zyppy™ Plasmid Miniprep Kit was easy to use and gave high clean concentration of the vector."

"The Zyppy™ Plasmid Miniprep Kit is an amazing one, which offers not only high quality plasmid DNA but also good quantity. Most of the time it gave good quality and quantity of DNA and it works better than the Qiagen Miniprep Kit. So the downstream DNA sequencing of the plasmid DNA is very good. Also with this kit the plasmid DNA isolation is very easy with the columns and most importantly I can easily monitor the lysis with the blue dye. Very very important for me is the price is way cheaper than Qiagen Miniprep Kit for the same or better quality plasmid DNA isolation. Our lab saves so much amount of money every month by using this kit. Also the customer care is good in terms of technical support."

"The product is easy to use and the low elution volume columns are great if you need high concentration plasmid from low-copy number plasmids."

"Our yield is decent as well and the quality of the yield is good for our downstream applications. The pricing is great."