Status:
ReviewerMember since: 2020
Organization: Icahn school of Medicine at Mount Sinai
The best plasmid miniprep kit
Application Area: plasmid miniprep isolation
"This kit is easy to use and time efficient and has the best value for money. I have been using this for the past 10 years. Highly recommended."
Status:
ReviewerMember since: 2022
Organization: University of California, Riverside
Quick, easy to get great result!
Application Area:For extracting plamids
"This product is very good, quick, easy to use and I can easily get high quality plasmids. I love it!"
Status:
ReviewerMember since: 2022
Organization: Rice University
Consistent and great results.
Application Area:Molecular Biology
"Initially, I was using more expensive plasmid prep kits from other manufacturers and someone introduced me to a much more cost-effective zippy kit. I was a bit apprehensive about its yield and quality but decided to give it a try. And you know the results were quite amazing and even better than any other kit I ever used before. It's been 5 years and now I and my whole lab are completely reliant on the Zippy mini prep kit. It never disappointed us. "
Status:
ReviewerMember since: 2022
Organization: UCDavis
Friendly protocol. Very efficient. Fast.
Application Area:Plasmid isolation
"Consistently good recovery of plasmid DNA, easy to use and fast."
Status:
ReviewerMember since: 2022
Organization: Oakdale High School
Effective time saver. Great results for downstream applications.
Application Area:Education
"Yet again, this Miniprep kit has given me and my students valuable time back in the lab. If it wasn't for the streamlined effective protocol, I would have never finished my lessons in time."
Status:
ReviewerMember since: 2022
Organization: University of South Alabama
Great results compared to other brands
Application Area:Extract plasmids from E.coli
"High quality plasmids, high yield, quick and easy-to-follow protocol"
Status:
ReviewerMember since: 2020
Organization: Masonic Medical Research Institute
Fast Isolation of High Quality Plasmid Every Time
Application Area:Plasmid Isolaiton
"I use this kit exclusively now for isolation of all my plasmids. The protocol is fast, easy, and always yields large amounts of high quality plasmid for a MINIprep kit. I find myself doing alot less MIDIpreps now. I recommend this kit to all of my colleagues."
Status:
ReviewerMember since: 2019
Organization: UT Health San Antonio
If the shelf life little longer would be better.
Application Area:For cloning
"This product is easy to using, also the price good, but not stable, and can not keep longer, shelf life is too short."
Status:
ReviewerMember since: 2022
Organization: UMASS medical school
Great kit for fast purification.
Application Area:Purify plasmids is much easy and fast using this kit.
"In our lab we use many plasmids constructs and we perform purification daily. This kit is amazing and safe us time and money without drop quality."
Status:
ReviewerMember since: 2021
Organization: Oakdale High School
Saves so much time! Turns a two day lab into one day lab.
Application Area:Education
"I am a high school AP Biology teacher. I have only so much time with my young scientists everyday. The Zyppy Plasmid Miniprep kit gave me an extra day every time I used it. On average we do three or four minipreps every year (per class per year). That equates to about three days for more instruction and more downstream applications for plasmid biology, whether it be additional transformation or restriction analysis. The protocol is very streamlined and easy to understand. My 16 and 17 year old students (and some as young as 15) have no problem completing the protocol and getting reproducible results. I've received nothing but spectacular customer service and I as far as value is concerned, I buy this time and time again."
Status:
ReviewerMember since: 2022
Organization: UW-Madison, School of Pharmacy
Great results and money saving!
Application Area:Plasmid purification for mammalian cell transfection
"This kit is very easy to use and time-saving. More importantly, it's relatively cheap compare to other vendors."
Status:
ReviewerMember since: 2021
Organization: University of Hawaii
Overall would recommend!
Application Area:Plasmid extraction
"Great quality results with easy protocol. Good value and good customer service!"
Status:
ReviewerMember since: 2021
Organization: CSIR-IICT HYDERABAD
Have obtained great results till date, still using.
Application Area:Cloning purpose
"Effective and easy to use, recommended for faster extraction of high quality DNA."
Status:
ReviewerMember since: 2021
Organization: Washington State University
Awesome results.
Application Area:Plasmid cloning and extraction of plasmid dna
"This product is time efficient and very easy to use."
Status:
ReviewerMember since: 2019
Organization: National Institute Of Research And Physical-chemic
Error free visualization and easy for research.
Application Area:Separate plasmid DNA from E
"The Zyppy™ Plasmid Miniprep kit modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, then purify using our Fast-Spin column technology. Additionally, the innovative colored buffers included in the kit permit error-free visualization identification of complete bacterial cell lysis and neutralization. Our Zyppy™ Plasmid Miniprep Kit is the fastest and simplest method available to efficiently separate plasmid DNA from E. coli. It is a powerful and innovative product."
Status:
ReviewerMember since: 2020
Organization: University of Maryland
Remarkable results with high quality and quantity
Application Area:Gene cloning
"I have used Qiagen products for more than 20 years. I think that Qiagen is the number one company in terms of quality control. I had a chance to use Zymo columns, and I realized that Zymo is completely compatible with Qiagen. I am really happy with the quality and ease of use. After extraction of DNA quantity is above 100 ng/ul, which is a remarkable outcome. I suggest you use Zymo systems in your lab."
Status:
ReviewerMember since: 2016
Organization: Iowa State University
Excellent quality product and great savings for the lab much more so than other brands
Application Area:Plasmid DNA extraction
"The kit produces excellent quality and quantity plasmid DNA (even with low copy). Very easy to handle like any other brand. We use it mainly for sequencing. The sequencing results always come out excellent. Never had any problem. Used it for the last 12 years. The best part of it: It is 2 times cheaper than the other brands and still it offers the best quality outcome. I highly recommend for all labs to use it."
Status:
Advanced ReviewerMember since: 2018
Organization: MRC Laboratory of Molecular Biology
Speedy miniprep workout
Application Area:Routine plasmid DNA minipreps
"It is a handy miniprep kit with a big accentuation on the speed of processing samples. To me, it has two advantageous: - no need to centrifuge cell pellets as you add lysis buffer directly into the media; - there is something in the neutralization buffer that helps to stick all of the debris together. One disadvantage: as opposed to the Qiagen kit it is sensitive to the amount of cells you use."
Status:
Advanced ReviewerMember since: 2019
Organization: University of Chicago
Reliable prep with high quality DNA
Application Area:Screening recombinant clones
"Overall, this is one of the more efficient and reliable mini preps I have used. I routinely obtain high quality DNA, good enough for sequencing as well as transfections of immortalized cell lines. My final concentration is approximately 250 ng/µl."
Status:
ReviewerMember since: 2021
Organization: Berkeley
Good yield, good value for money.
Application Area:Purify DNA for cloning
"This product gives me good yield. I mostly use it to validate cloning constructs."
Status:
ReviewerMember since: 2021
Organization: UW-Madison, School of Pharmacy
Great results, fast and cheap.
Application Area:Plasmid purification for mammalian cell transfection
"The plasmid purification kit is very rapid, easy to use. Also, the plasmid quality and yields are high. Moreover, this kit is like 50-100 dollars cheaper than what we used before."
Status:
ReviewerMember since: 2021
Organization: Northwestern University Chicago
Great results, reproducible, cost effective.
Application Area:Preparation of plasmids for cell culture transfection
"The plasmid isolation kit from Zymo is cost effective, easy to use and quick. The reproducibility and reliability of the kit is awesome. I recommend using the kit as it is equally efficient to many other manufacturers, and is cheaper too."
Status:
Advanced ReviewerMember since: 2021
Organization: Federal university of Minas Gerais
Fast and high quality.
Application Area:Bacterial cloning
"Easy and fast protocol, I was able to get good plasmids concentrations with high purity."
Status:
ReviewerMember since: 2021
Organization: Indiana University
Works great, fast kit, good plasmid yield.
Application Area:DNA purification
"The kit is incredibly easy to use, and faster than most kits on the market. The Zymo sales team has always been incredibly friendly and helpful. The value for money is excellent as the kit is quite affordable."
Status:
ReviewerMember since: 2021
Organization: Brown University
Great value for money, consistent results.
Application Area:Purification of bacterial plasmid DNA
"This kit offers great value for money, and gives consistently pure and high quality DNA for each prep. There is little variation between preps, and the DNA is immediately usable for other transformations in the lab. It is also very easy to use, with clear instructions, so it takes a very short time to prep many plasmids."
Status:
ReviewerMember since: 2021
Organization: Donald Danforth Plant Science Center
Incredible - will redefine how I teach biotech in my classroom!
Application Area:Education
"I did not purchase this miniprep kit, rather I received it as a sample. I really could not be happier with this kit. Because of it's "Pellet-Free" protocol, it saves me so much time that I could easily get purified plasmids within a 45 minute high school period. This really opens up the possibilities and flexibility for my students and allows them to work closer to their own pace. With traditional miniprep kits, I have to have all 8-10 lab groups be on the same page during the protocol. With Zyppy's fast procedure and reduced spin times, my kids can have freedom to get ahead or take their time to gain a better grasp on the science going on in that tube/column. Ease of use: simple: straightforward protocol After-sales care: had an senior account executive reach out to me the next day to ensure proper address and information. Value: Time is money during a high school lesson. Because I can have my kids get a prep done within ten minutes so they can perform their transformations/restriction digests within a period, I would happily pay the $0.83-$1.30/prep. "
Status:
ReviewerMember since: 2021
Organization: University of South Alabama
Great results and straightforward.
Application Area:Molecular biology
"This kit is very easy and straightforward to use. It is one of the cheapest in the market as well. "
Status:
Advanced ReviewerMember since: 2021
Organization: Sam Houston State University
My go to kit for this type of application.
Application Area:Plasmid amplification
"Very efficient and easy to use kit! Would highly recommend!"
Status:
ReviewerMember since: 2021
Organization: Sam Houston State University
Great results, would not think of using other plasmid miniprep kits.
Application Area:Viral research
"This plasmid miniprep kit works great for our lab, we always get great results using this prep kit. These kits always come in on time as well, we have never had any problems with the shipping or storage of this product. I have used these specific kits several times, high yield of results, and very consistent results. We will continue to use and choose these plasmid miniprep kits over others because of the great results, ease of storing and usage, and the value of the product. The Zyppy Plasmid Miniprep Kit is a valuable and non-replicable product for our laboratory."
Status:
ReviewerMember since: 2021
Organization: OSU
Great results, we purchased more!
Application Area:Plasmid preparation
"It has high productivity and high quality. It is very easy to use and cheap. We like it very much!"
Status:
ReviewerMember since: 2021
Organization: UCLA
Fast, reliable, cost-effective.
Application Area:Routine minipreps for colony screening
"We heavily use Zyppy minipreps for our routine cloning work. When purifying plasmid DNA for sanger sequencing or gel electrophoresis, it gives consistently good yields and simplifies / speeds up our workflow."
Status:
ReviewerMember since: 2020
Organization: University of California, Riverside
Great price for quality.
Application Area:Plasmid isolation for molecular biology
"I love the quality/price ratio. These work great for lab members, and they are cheap enough that I don't mind letting undergraduates practice with them."
Status:
Advanced ReviewerMember since: 2021
Organization: Marine Biological Laboratory
Great reproducible results + reasonable price.
Application Area:Genomics
"Zyppy plasmid kit is really easy to use and quick, value for money is superb, great reproducibility."
Status:
ReviewerMember since: 2021
Organization: Colorado State University
Great results and great value.
Application Area:Extract plasmids for cloning
"This kit is easy to use and competitive with pricing. Instructions are easy to follow. We get good yields for all plasmids we use with the kit."
Status:
ReviewerMember since: 2021
Organization: Yale university
Good quality plasmids for sequencing and downstream application.
Application Area:Molecular biology
"Higly efficient, reproducible, less hands on time and economic."
Status:
ReviewerMember since: 2020
Organization: GRIP Molecular Technologies
Awesome product and treats in the box!
Application Area:Purification of plasmids for protein expression and sequencing
"My entire experience with Zymo has been positive. Their products are the best value, are reliable, and reproducible. I have set up several labs over my career and use Zymo products in each of them."
Status:
ReviewerMember since: 2021
Organization: Drexel University College of Medicine
Always works! That's important!
Application Area:Cloning
"Zyppy is easy to use! And I like that I can choose from 600 µL culture or from more volume to spin-down. We get 800 preps - no worry about running out soon."
Status:
ReviewerMember since: 2016
Organization: University of Tennessee
Consistently great results!
Application Area:Plasmid DNA extraction
"We have been purchasing this product for over 5 years now and have to say the consistency between lots if outstanding. I highly recommend this Zyppy plasmid miniprep kit."
Status:
ReviewerMember since: 2020
Organization: GRIP Molecular Technologies
No-brainer choice for minipreps.
Application Area:Synthetic biology
"This is my go to miniprep kit. Incredibly affordable, great results every time, and candy in the box. I've set up three research labs and Zymo products are one of the easiest supplier choices I make."
Status:
ReviewerMember since: 2020
Organization: Icahn school of Medicine at Mount Sinai
Almost use it daily!
Application Area:Prepare plasmid DNA from E coli
"Zyppy plasmid mini kit delivers a quick and easy way to isolate plasmid DNA of good quality that is compatible with routine molecular biology application. After using for over 10 years, I can't live without it and have stopped using plasmid mini prep kits from any other supplier. I have not contacted the after sale care team about this product, because it has not given me any problem! highly recommended!"
Status:
ReviewerMember since: 2020
Organization: Synvivia Inc
Good value, consistent results.
Application Area:Molecular biology for library fabrication
"Zymo kits have been the most consistent of any brand we've tried. They're also very good value. We like the lack of pelleting cells in the Zyppy kit and proceeding directly to lysis of culture. This saves time and to our surprise, the yield wasn't substantially lower."
Status:
ReviewerMember since: 2020
Organization: MSU
Would recommend all Zymo products!
Application Area:Cloning and transformation
"Easy to use with clear instructions. Quick to perform and food quality plasmids for downstream applications."
Status:
ReviewerMember since: 2020
Organization: University of Cincinnati
My favorite plasmid prep kit! Made my post doc lab get it!
Application Area:Cloning
"I achieved reproducible results and the kit is quick for getting plasmid DNA for cloning and downstream applications."
Status:
ReviewerMember since: 2020
Organization: University of Florida
Affordable plasmid miniprep kit.
Application Area:Gene cloning
"Great results for the price. It is really fast and easy to perform plasmid DNA extraction."
Status:
ReviewerMember since: 2020
Organization: Abalone Bio
Best miniprep kit.
Application Area:Plasmid miniprep
"This kit is quick and simple, and gives good plasmid yields every time. Other cheap kits have extra steps, extra columns, or the column material flakes out and contaminates the preps. The Zymo kit works as well as the expensive kits."
Status:
ReviewerMember since: 2020
Organization: University of Colorado
Fastest, easiest, most consistent miniprep kit I've used
Application Area:Plasmid extraction from E coli
"Extremely easy to use! Color change for neutralization is helpful. No problems with debris not centrifuging down as with some other kits. High yields and consistent results."
Status:
ReviewerMember since: 2020
Organization: Wingate University
Good results and ease of use for the money.
Application Area:Undergraduate course based research experience
"I use this product every semester, with 48 sophomore/junior undergraduate students. The students are all able to successfully isolate their individual engineered plasmids, most with high concentrations. I personally have color coded the items in the kit and the instructions associated to help make the instructions more transparent and visible for my novice users to follow."
Status:
ReviewerMember since: 2019
Organization: Promab Biotechnologies Inc.
Great kit!
Application Area:DNA extraction
"This is by far the easiest to use and best value miniprep kit I have used. It produces great quality plasmid DNA."
Status:
ReviewerMember since: 2019
Organization: Touro University Nevada
Fast, reliable plasmid isolation mini-prep kit.
Application Area:Isolate plasmids for transfection
"I started using Zyppy Plasmid kit from a free sample and I am hooked. It is so fast and easy to use! I have used the ZymoPure miniprep kit as well. Both work well, though Zyppy is faster. I recommend this kit because of its speed. The quality of the DNA plasmids that I recover is also good."
Status:
ReviewerMember since: 2019
Organization: Institute of Molecular Genetics
Easy to use, fast, reproducible, and good quality
Application Area:Plasmid purification for in vitro studies and cloning
"Have used this product for years. Very easy to use, coloured buffers help to visualize neutralisation, high yield, good reproducibility, plasmids can be used for in vitro studies and for cloning as well, and it works so fast (this parameter is very important for me)! Almost all steps require just 15 seconds of centrifugation – usually, I spin down 4ml of culture and add 500ml of mQ to get 600ml final volume to start plasmid purification."
Status:
ReviewerMember since: 2019
Organization: Csu
Very simple to get high concentration
Application Area:Molecular cloning
"The product offers simple direction and ease of use, so we are able to give it to the entire lab to use without a problem"
Status:
ReviewerMember since: 2019
Organization: UT Health San Antonio
If you make your product better we will buy it again.
Application Area:E. coli bacteria for cloning
"The DNA concentration was not high, your price is good, but we can't buy large kits because the shelf life of the kit is very short. They always expire so quickly we can't keep them longer."
Status:
ReviewerMember since: 2019
Organization: University of Rochester
Easy to use, reliable and fast.
Application Area:Plasmid isolation from E. Coli
"This product provides the fastest way to isolate DNA plasmids from E. Coli. It is reliable, easy to use and the purified plasmids are endotoxin-free and ready for transfection!"
Status:
ReviewerMember since: 2019
Organization: Chicago Medical School
Great results without any issues.
Application Area:Miniprep for DNA injection into C. elegans
"It is easy to use, and its quality is beyond expectation! We use plasmid DNA for the injection into C. elegans to generate transgenic lines without any further purification."
Status:
ReviewerMember since: 2019
Organization: University of Georgia
More than enough DNA.
Application Area:Metabolic engineering
"The kit is easy to use and provides plenty of plasmid DNA to use in down-stream work. It is cheaper than it’s competitors in the market."
Status:
Advanced ReviewerMember since: 2019
Organization: University of Chicago
Reliable and efficient DNA prep.
Application Area:Preparation of dNA to screen or sequence
"The Zyppy MiniPrep kit is an efficient spin based protocol for the production of plasmid DNA for most routine DNA applications, we have excellent results in both preparative approaches - subcloning, PCR as well as screening. Our favorite kit to date."
Status:
ReviewerMember since: 2019
Organization: Madurai Kamraj University, Dept. Molecular Biology, School of Biological Sciences
I have a chance to use kits for my experiments that give better results.
Application Area:Isolate genomic plasmid DNA from bacterial samples
"Very good product for isolation of genomic, plasmid DNA from bacteria. I achieved high quality yield and also reproducible results. I used the above mentioned product only in overseas labs (Israel). Easy to use and handle. Minimum number of solutions for better results."
Status:
ReviewerMember since: 2019
Organization: CSIR
Value for money for everyday use.
Application Area:Plasmid DNA isolation from E. coli
"Very easy to use. Plasmid quality is consistent, although not the best yield and concentration. Other, more expensive kits give higher quality DNA, but for everyday screening, this kit is value for money."
Status:
ReviewerMember since: 2019
Organization: INSERM
Good product, consistent results.
Application Area:Bactera transformation
"So far, this kit has been perfect. I have been able to easily make my minipreps, consistently."
Status:
ReviewerMember since: 2019
Organization: Cardiff University
Quickest and highest recovery - no brainer really.
Application Area:Protein expression
"I carried out a comparison against 2 other major brands of plasmid mini prep kit, purifying plasmid from 1ml of pelleted bacteria. The Zyppy kit was the quickest and cheapest per sample (at the time, I haven't compared prices since) of the three. In terms of yield, it also had the highest recovery of our plasmid, which is much larger than average. The kit is simple to use - my only warning being to make sure you add ethanol to the wash buffer when you receive the kit to prevent students using the concentrated solution by mistake. I have no experience of the after sales care."
Status:
ReviewerMember since: 2019
Organization: Providence College
The technical support is unbeatable!
Application Area:Prepare plasmids for transformation.
"The plasmid miniprep kit is very easy to use. On occasions when I have had difficulties and my students obtained poor results, I called tech support and spoke to Michelle and she was able to troubleshoot the problem for us. It's really great to know that whenever I have a problem, I can call tech support and obtain the help that I need."
Status:
ReviewerMember since: 2019
Organization: Promab Biotechnologies Inc.
Great miniprep kit, my go-to.
Application Area:DNA purification
"I have used many different kits to do minipreps and this one is the easiest and quickest I have used so far, and it is also cheap."
Status:
ReviewerMember since: 2019
Organization: STANFORD UNIVERSITY
Fast minipreps with high yield.
Application Area:DNA isolation
"Much better than Qiagen columns, with higher yield and a shorter protocol!"
Status:
ReviewerMember since: 2019
Organization: HHMI - Stanford
We use this product a lot. Zymo has replaced Qiagen in our lab.
Application Area:Molecular biology and glycomics
"Zymo products have helped us stay on budget, while providing exceptional products."
Status:
ReviewerMember since: 2019
Organization: University of South Carolina Aiken
Problem free.
Application Area:Molecular cloning
"This kit is easy to use and reliable. I run a primarily undergraduate laboratory and they rarely have any problems. We have recommended this kit to others at our university and they also have been pleased."
Status:
ReviewerMember since: 2019
Organization: ATUM
Easy and quick kit for plasmid DNA.
Application Area:Purify plasmid DNA from E. Coli for transfection
"The product is easy to use and provides very low level endotoxin plasmid DNA. Its also fast - I can complete 20 samples in under 20 minutes!"
Status:
ReviewerMember since: 2019
Organization: CELLSCRIPT, LLC
It just works.
Application Area:Plasmid stock for clone screening.
"Haven't needed customer support because the kit works, and works well. Very straight forward and simple protocol. Plasmid is ready in a timely fashion. Have not had any problems with the kit ever."
Status:
ReviewerMember since: 2019
Organization: Ruhr Universität
Fast and easy at a good price.
Application Area:Plasmid purification
"Fast and easy to use with colored buffers to show when the lysis and neutralisation steps are complete. High yield and the price is very good."
Status:
ReviewerMember since: 2019
Organization: University of Georgia
Good result.
Application Area:Cloning for In situ hybridization
"Even though yield is little bit low, quality of plasmid prep is good."
Status:
ReviewerMember since: 2019
Organization: Stellenbosch University
Great.
Application Area:Yeast plasmid extraction
"High quality DNA concentration. Higher yields when buffer or water is warm. Easy to use."
Status:
ReviewerMember since: 2019
Organization: Emory University Department of Human Genetics
Excellent product, would be great to see it in a 96-well format.
Application Area:Isolation of plasmids to screen clones
"This product worked great everytime with clear directions. The only reason for a 3 star after sales care score was because I didn't need any after sales instruction. I will continue to use this kit for restriction digest screening of clones."
Status:
ReviewerMember since: 2019
Organization: University of Stuttgart - Institute for Technical Biochemistry
I cannot think of any reasons why I should buy any other kit.
Application Area:Enzyme engineering
"I appreciate the easy use of the kit and the purity of plasmid DNA after preping out of E. coli cells. Even students can use the kit without anything else than the provided manual and get very good results. I think it would be even a bit better, if there were small symbols or something like that on the coloumns to identify them in the centrifuge without having to write on the very small labelling areas. The numbers given there are very small and not so easy to read (actually I'm not even sure, if they are meant for identification or if they are just remains from the production process)."
Status:
ReviewerMember since: 2019
Organization: Molekulare Physikalische Chemie
Perfect.
Application Area:Analysis of CpG methylation in urothelial cancer genes
"I have performed hundreds of mini preps. I can say the following: very easy and intuitive handling, reproducible results in terms of quality and concentration acceptable price and benefit."
Status:
ReviewerMember since: 2016
Organization: Augustana College
Easy to use and effective
Application Area:Gene cloning applications
"The product was very easy for my undergraduate students to use and understand. Consistently high-quality samples were obtained."
Status:
ReviewerMember since: 2016
Organization: academia
The only mini I choose to purchase!
Application Area:Plasmid propagation
"Easy application, fool-proof for beginners. Great cost and time!"
Status:
ReviewerMember since: 2016
Organization: Cleveland State University
Always order this from here! Great results.
Application Area:Isolate plasmids
"It is very easy and fast to use. It gives great results."
Status:
ReviewerMember since: 2016
Organization: ANSES
Fast and easy-to-use
Application Area:Plasmid purification
"Very fast; no isopropanol precipitation step. Easy to use; few steps and colored buffers to check if each step was run successfully. Great DNA yield and quality."
Status:
ReviewerMember since: 2016
Organization: California Lutheran University
Fast kit, reproducible results
Application Area:Prepare plasmids for transformation/protein over-expression in E. coli
"We grow a larger overnight culture, then pellet the cells and resuspend in 600 uL to prepare as directed. This does an excellent job of creating high concentration of pure plasmid that reliably works for us in transformation as well as mutagenesis."
Status:
ReviewerMember since: 2016
Organization: Thermo Fisher Scientific
Convenient and fast, would like lids though.
Application Area:Cloning for protein identification and expression
"The Zyppy Plasmid Miniprep kit was the first kit that I used that has a color indicator added to show when lysis has been fully neutralized. Because of this feature, the quality and yield of the DNA is better than what I experienced in earlier kits from at least 4 other brands. Some steps have been made more convenient, e.g. there is no need to empty the collector tube between the 2 wash steps. These little things make the entire process just that little faster. I don't like that you have to centrifuge the spin columns without a lid. A tip for resuspending the cells: add only 300 uL first, rash the tubes over the top of a tube rack and then add another 300 uL and the 100 uL lysis buffer. The method doesn't work as well with the 600 uL at once."
Status:
ReviewerMember since: 2016
Organization: UCSD
Great isolation plasmid kit
Application Area:Cloning of different gene fragment, PCR sub cloning,
"The kit can provide fastest way to get plasmid DNA. Also it is easy to use with highly reproducible results. The instruction is quite simple and user-friendly. The price of the kit is very competitive with other similar kits."
Status:
ReviewerMember since: 2016
Organization: UCSF
Quick n Easy
Application Area:Molecular Biology
"The Zymo Miniprep kit is extremely easy to use, takes less time than most other kits and gives great results!"
Status:
ReviewerMember since: 2016
Organization: Tulane National Primate Research Center
Great yield with minimum starting material
Application Area:DNA Isolation
"I would give this product 5-star rating. This kit requires less starting material, yield is great in less time and the quality is fantastic. I never get spark when I perform electroporation. Wonderful products."
Status:
ReviewerMember since: 2016
Organization: School of Medicine, University of Maryland
Streamlined pellet free miniprep.
Application Area:Plasmid purification for cloning and transfection purposes
"Zyppy Plasmid Miniprep Kit has removed the cumbersome step of pelleting the bacterial culture before the lysis step. Surprisingly enough the yield is good enough for all the downstream applications that one might need the purified plasmid for. It has made life easier!"
Status:
Advanced ReviewerMember since: 2016
Organization: University of Michigan
Great kit and save money and time.
Application Area:iPSC cell and neural cell differentiation
"That kit is good, high yield and purity is good, I am satisfied with it."
Status:
ReviewerMember since: 2016
Organization: Tytgat Institute AMC
Allows rapid and very efficient plasmid screening.
Application Area:Screening clones for correct plasmid
"The Miniprep kit is very easy to use. The isolated plasmid is very pure and can be used for all subsequent steps."
Status:
ReviewerMember since: 2016
Organization: University of Michigan Medical Center
Easy to use, quick and reliable
Application Area:DNA purification
"Easy to use, quick and reliable plasmid mini-prep kits. And in fact we use Zymo Midi and maxi prep kits as well as the pcr clean kits and RNA clean kits"
Status:
ReviewerMember since: 2016
Organization: Providence College
Undergrad-proof with excellent and quality yields.
Application Area:Isolate plasmids from bacteria.
"My undergraduate students use this miniprep kit to isolate all the plasmids we routinely use in the lab. It is undergrad-proof, with excellent and quality yields!"
Status:
ReviewerMember since: 2016
Organization: University of Calgary
Fine product, price a little too high.
Application Area:DNA purification (plasmids)
"Excellent product that produces high quality plasmid preps and is easy to use. However, in Canada, it is not competitive with several other products (BioBasic, FroggaBio) based on price. Cheaper than Qiagen last time I checked, and about the same as NEB Monarch."
Status:
ReviewerMember since: 2016
Organization: NCSU/UNC Joint Biomedical Department
A consistent mini prep kit for undergraduates and graduates alike.
Application Area:Plasmid Isolations
"Ease of Use- I rated this as mainly on my own wet lab experience using Zymo and Qiagen kits and also teaching to undergraduates. Yields from DH5 alpha E coli so far have been in acceptable ranges (100-300 ng/ul , 260/280 ratios near 1.7,1.8). Some optimizations and changes can be made like using water instead of the final elution buffer (for convenience). The kit is easy to show undergraduates and they can perform the mini preps within a week reliably. The color changes are consistent and comparable to other kits in ease. After-Sales Care- I rated this mainly on shipping and ordering. We receive shipments intact and on time when we order from the Zymo site. No shipping issues so far. Value for Money- I rate this mainly on my ordering experience in the lab. The price is comparable to other kits."
Status:
ReviewerMember since: 2016
Organization: Weill Cornell Medical College
Hands down the best miniprep I've ever used... and the yield is anything but mini.
Application Area:Plasmid purification for sequencing and transfection
"This prep is super fast, and gives a seriously large amount of transfection-quality DNA from a small culture volume. If I put all the bugs from a 3ml overnight through this kit I've gotten up to 35µg of DNA in a 10 minute prep. I basically don't need any other prep for routine transfections."
Status:
ReviewerMember since: 2016
Organization: Faculty of Agriculture, University of Zagreb
Really happy about how efficient is the extraction and the purity of the product.
Application Area:Extraction of plasmid DNA
"I got the "Zyppy™ Pasmid Miniprep Kit" to try it out. I must say that Zymo did not fail me, once again! The plasmids that I have extracted were pure and in record time. I know that the price for the kit is very competitive and even more economically friendly. The buffers have normal life span and are kept at room temperature and some are kept at 4 degrees. Neutralization buffer already has RNase inside so you don’t even have to make that post treatment afterwards."
Status:
ReviewerMember since: 2016
Organization: Old Dominion University
I won't even consider buying another plasmid kit.
Application Area:Plasmid minipreps for PCR and cloning.
"This kit is fast, simple and spot-on. We have not had a dud yet that wasn't due to UE (undergraduate error). As a matter of fact, we purchase the kit in the large (400 column) size on a regular basis. The buffers that come with the kit are ample and have a long shelf life. Zymo also helpfully sells the columns separately, so you can get the most value for your money and use all the elements so that nothing goes to waste. On the other hand, you can also buy the buffers separately, so if something mysteriously happens to one of the buffers (as in ending up all over the floor), you can rest easy that you can purchase just the spilled item and not have to buy an entire kit for one component. I've had great results with Zymo technical support and they don't try to upsell you on something that doesn't fit your needs. Oh, yes, and the Miniprep Plasmid Kit is inexpensive (really inexpensive!)."
Status:
ReviewerMember since: 2016
Organization: Univ of California San Francisco
Great value, can't live without this kit.
Application Area:Purify plasmid DNA from bacterial culture
"Fast, without the hassle of taking multiple Eppendorf tube in and out of microcentrifuge for spinning. The quality of final plasmid DNA is as good as Qiagen QIAquick Plasmid Miniprep Kit."
Status:
ReviewerMember since: 2016
Organization: Institute of Molecular Biology
Fastest, easiest, and most cost effective.
Application Area:Molecular cloning of DNA constructs.
"These are the fastest, easiest, and most cost effective plasmid prep kits we've tested. Importantly they require the least amount of peripheral equipment, e.g. centrifuges/tubes, vacuum pumps, etc."
Status:
ReviewerMember since: 2016
Organization: Florida State University
Great product.
Application Area:Study phosphorylation cell culture
"Easy to use, everything is clear. Always get great DNA yield. Fast protocol leading to fast results. Consistent results. "
Status:
ReviewerMember since: 2016
Organization: Tel Aviv University
It will be very hard to convince us to switch to other products.
Application Area:DNA purification for downstream protein expression
"This is one of the best products in the category! Best value for money, easy to use, reproducible yield and high quality DNA. Our sequencing issues were solved by using these kits."
Status:
ReviewerMember since: 2016
Organization: University of Houston
Ideal results! Little time involved with high quality.
Application Area:Plasmid isolation
"The Zyppy™ Plasmid Miniprep Kit was easy to use and gave high clean concentration of the vector."
Status:
ReviewerMember since: 2016
Organization: Iowa State University
It is a high quality & excellent product with a cheaper price.
Application Area:Preparation of plasmid DNA from bacterial culture
"The Zyppy™ Plasmid Miniprep Kit is an amazing one, which offers not only high quality plasmid DNA but also good quantity. Most of the time it gave good quality and quantity of DNA and it works better than the Qiagen Miniprep Kit. So the downstream DNA sequencing of the plasmid DNA is very good. Also with this kit the plasmid DNA isolation is very easy with the columns and most importantly I can easily monitor the lysis with the blue dye. Very very important for me is the price is way cheaper than Qiagen Miniprep Kit for the same or better quality plasmid DNA isolation. Our lab saves so much amount of money every month by using this kit. Also the customer care is good in terms of technical support."
Status:
ReviewerMember since: 2016
Organization: university of california, san francisco
Easy to use, affordable.
Application Area:Extract plasmid from bacterial cultures
"The product is easy to use and the low elution volume columns are great if you need high concentration plasmid from low-copy number plasmids."
Status:
ReviewerMember since: 2016
Organization: USC
Bang for the buck.
Application Area:Immortalized cell lines & tissue.
"Our yield is decent as well and the quality of the yield is good for our downstream applications. The pricing is great."
Status:
ReviewerMember since: 2016
Organization: Public Health Agency of Canada
Excellent product.
Application Area:Plasmid isolation.
"It’s super easy to use, faster than most products on the market and better recovery of plasmid from prep."
Status:
ReviewerMember since: 2016
Organization: The Scripps Research Institute
Affordable and easy to use
Application Area:Molecular Biology
"Pretty easy to use with a color based reagents. At the same amount of final material compared with other brands however the rapidness and easiness of the technique makes Zymo products a very good choice."
Status:
ReviewerMember since: 2016
Organization: University of California, SF
Fast purified plasmids
Application Area:Molecular biology
"I use Zyppy Plasmid Miniprep kit to replace vendor Q miniprep. Zyppy Miniprep is fast and easy."
Status:
ReviewerMember since: 2016
Organization: University of Massachusetts Medical School
Zyppy miniprep kit gives reproducible results and it's very easy to use!
Application Area:Extract plasmid DNA
"I can always expect high quality and reproducible results from using Zyppy miniprep kit! Best part is that endo wash step allows in vivo use in downstream experiments."
Status:
ReviewerMember since: 2016
Organization: University of Pennsylvania
Easy to use, fast results, good price and friendly customer service.
Application Area:Cloning and subcloning for extracting Endotoxin free DNA.
"I have been using this kit since it became available - it is the most easy to use kit I have ever used. It delivers endotoxin free DNA for me to sequence. It only takes about a few minutes to get what I need. The price is very good as well. I have recommended it to many of my friends in biomedical research field."
Status:
ReviewerMember since: 2016
Organization: NCI
This kit is fit for plasmid sequencing!
Application Area:Methylation examination for ES cell
"Plasmid extraction is easy and fast! The cost for every sample is low!"
Status:
ReviewerMember since: 2016
Organization: krannert Institution of Cardiology, Indiana University
Great product, we've been using it for years and this product has never let us down.
Application Area:Plasmid DNA extraction and purification
"This kit is easy to use and produces good yields with high quality. It is time efficient and well worth the money."
Status:
ReviewerMember since: 2016
Organization: University of Illinois
Great value for the money, and saves time!
Application Area:Plasmid extraction and cloning
"Miniprep kit from Zymo is great because it takes less time compared to some other kits. The yield and purity of plasmid prepared with this kit are good and comparable to other brands."
Status:
ReviewerMember since: 2016
Organization: IBBR/CNR
Great results with the right price.
Application Area:Quick preparation of plasmid DNA
"Very easy procedure starting with very low amount of cells. Surprising DNA yield with very good quality for the next experimental steps (260/280 OD value, sequencing, restriction analysis, PCR, etc...)."
Status:
ReviewerMember since: 2016
Organization: GigaGen
High yields, great price
Application Area:Plasmid preparation
"This plasmid prep kit works great. We get higher yields than with the QIAGEN kit, it is easier and also cheaper. Zymo Tech Support is also helpful. "
Status:
ReviewerMember since: 2016
Organization: University of New Mexico Health Science Center
Awesome kit!
Application Area:Plasmid DNA purification
"We love this product! It's fast, reliable and always produces clean and high quality DNA. This kit is way cheaper than competitor brand. We have basically transitioned all our plasmid DNA isolation kits to Zymo products!"
Status:
ReviewerMember since: 2016
Organization: CEITEC MU
Great product and good price-quality relationships
Application Area:Plasmid DNA isolation
"Cost effective. Quick protocol and Good yield. DNA is of good quality for further cloning procedures."
Status:
ReviewerMember since: 2016
Organization: Augustana College
Excellent results from a fast kit
Application Area:Isolate plasmid DNA from bacterial samples
"This product and fast and easy to use. My undergraduate students had no problems following the short protocol to obtain high quality plasmid DNA. I would definitely purchase this kit again."
Status:
ReviewerMember since: 2016
Organization: Rosalind Franklin University of Medicine and Science
Great performance. Easy protocol. Transfection quality plasmid DNA.
Application Area:Plasmid DNA purification
"Great performance! The pellet-free modified alkaline lysis procedure is very cool and fast! The protocol is relatively easy and straight forward. Transfection quality plasmid DNA. I have switched to this product a couple of months back and am happy about it!"
Status:
ReviewerMember since: 2016
Organization: Icahn School of Medicine at Mount Sinai
Really great- have switched to Zymo for all kits
Application Area:purify plasmid DNA from bacterial culture for sequencing and/or transfection
"The product is easy, reliable and efficient. The cost per column is also significantly better than QIAGEN, although Zymo recently has raised their prices. I like how you can go directly from culture in this kit, although I never use just 600ul (typically I spin 5mL of culture down and resuspend in PBS). "
Status:
ReviewerMember since: 2012
Organization: Academia Sinica
Best mini-prep kit in the market
Application Area:cloning and gene manipulation
"Compare to other company's product, it is cheaper. Easy to use. If you have many samples, it can save you a lot of time."
Status:
ReviewerMember since: 2016
Organization: academia
It's the go-to kit for my lab!
Application Area:purify plasmids, bacterial dna preparation
"It is easy to use, protocol is direct and easy to follow, and the purification of the plasmids always results in a high quality. Due to the time it takes to use the kit and the ease of instructions I always teach my undergraduate students how to perform MiniPreps using this kit only! It's approachable and quick! Love it!"
Status:
ReviewerMember since: 2016
Organization: University of Tennessee
Love this kit! Very quick and easy to use.
Application Area:DNA Isolation/purification
"Love this kit! Very quick and easy to use. We receive good quality DNA which we use for qPCR."
Status:
ReviewerMember since: 2016
Organization: NCI, NIH
Great results, I would use it again
Application Area:plasmid isolation from 4 ml bacterial cultures
"It’s easy to use, offers great yield and quality DNA"
Status:
ReviewerMember since: 2016
Organization: Umich
Great product!
Application Area:Growing CRISPR plasmids for transfection.
"Fantastic! Have used it for 5 months with no issues. Equivalent to QIAGEN and much cheaper! "
Status:
ReviewerMember since: 2016
Organization: Clark University
Very efficient and easy to use. High quality results obtained reproducibly.
Application Area:Miniprep of plasmid isolates
"The Miniprep kit is very user friendly and easy to use. Gave me very high quality results and a pretty good yield of DNA which I easily used for cloning and other downstream applications. The kit is also very less prone to contaminants and involves fewer cumbersome steps as often found in other Miniprep kits. So thank you very much."
Status:
ReviewerMember since: 2016
Organization: University of Michigan
Fastest kit, consistent and great results
Application Area:DNA preparation
"I love all Zymo kits. They are fast, easy to use and reliably give good results."
Status:
Advanced ReviewerMember since: 2015
Organization: The Institute of Cancer Research
Glad to know this brand from my colleague, save lots of time and still give me very good quality and yield of plasmids
Application Area:Plasmid preparation
"Easy to use, quick procedure without any incubation time, good yield and quality for further experiments. Good value. Highly recommend."
Status:
ReviewerMember since: 2015
Organization: The Ohio State University
Great product. The savings mean we will never go back.
Application Area:Make plasmids for protein expression
"These kits work great and are easy to use. The DNA is as clean if not cleaner than the "name brand" kit we used to use. The price is a huge bonus. We won't go back."
The Zyppy™ Plasmid Miniprep Kit features a pellet-free modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures.
Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify using the provided Fast-Spin column technology. Additionally, the innovative colored buffers included in the kit permit error-free visualization and identification of complete bacterial cell lysis and neutralization. The Zyppy™ Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. coli efficiently.
The plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.