Status:
ReviewerMember since: 2021
Organization: Manipal School of Life Sciences, MAHE
Great product. Worth the investment!!
Application Area: Methylation studies
"The Taq PreMix is a wonderful product that has been used in our lab since a long time. We have been using the same for Bisulfite PCRs and also for Methylation Specific PCRs. The results are good and there is no issue with the polymerase at all. We are all delighted to have identified such a good product from the market for our methylation studies."
Status:
ReviewerMember since: 2021
Organization: SUNY Plattsburgh
Fast and effective PCR setup!
Application Area:Genetics
"ZymoTaq premix made our PCR fast and easy, so we could get results and focus on what matters! "
Status:
ReviewerMember since: 2021
Organization: University of Virginia
Easy to use chemistry with reproducible results!
Application Area:Methylation detection for forensic applications from blood samples
"The ZymoTaq DNA Polymerase was very easy to use along with the product information sheet available on the website. I have even successfully used this kit to compete half-reactions intended for use in microfluidic applications, and the chemistry appears to be sensitive with very reproducible results with down to 1 ng/µL of input DNA standard. The reaction setup is simple and straightforward and the technical support staff has been willing to walk me through it each time I have called. For methylation-specific PCR, I think Zymo provides the best products for the price. I recommend this chemistry to all of my friends and colleagues looking to begin projects in epigenetics research without breaking the bank."
Status:
ReviewerMember since: 2019
Organization: TruMe Labs
Great results; easy to use; better PCR than with many competitive formulations.
Application Area:Amplification of converted methylated DNA
"I like using ZymoTaq for amplification of methylated DNA after conversion. Compared to PCR enzymes from other vendors, ZymoTaq provides superior performance. Some of our targets cannot be amplified by anything else other than ZymoTaq."
Status:
ReviewerMember since: 2016
Organization: Erasmus MC University Medical Center Rotterdam
Excellent efficiency, recommend it for DNA methylation analysis!
Application Area:Amplify bisulfite-converted DNA templates
"I have been using this product for years now in all my epigenetic-based assays, which I develop for forensic applications. The product comes in the form of premix, therefore it is very easy and quick to use, cost-effective and mainly, has been working 95% of the time when designing new bisuflite-converted DNA primers. Researchers familiar with such analysis will understand how difficult sometimes is to efficiently amplify the difficult, T-rich nature of these converted templates."
ZymoTaq™ DNA Polymerase is a hot-start polymerase that is ideal for amplification of bisulfite-converted DNA. Because it is a heat-activated, thermostable DNA polymerase, ZymoTaq™ reduces primer dimer and nonspecific product formation, whereas conventional polymerases typically exhibit these problems with bisulfite-converted DNA templates. In addition to the amplification of bisulfite-treated DNA for methylation detection, ZymoTaq™ DNA polymerase can also be used for conventional PCR, and real time PCR. The enzyme also has 3-terminal transferase activity, making it ideal for use in TA-cloning by the addition of "A" overhangs to amplified DNA.