ZymoPURE™ II Plasmid Midiprep Kit by Zymo Research

Manufacturer Zymo Research  |   Model: D4200
4.6
/
5.0
  |  36 reviews
The fastest spin-column based procedure for purifying up to 300 µg of ultra-pure endotoxin-free transfection-grade plasmid DNA.


ZymoPURE™ II Plasmid Midiprep Kit by Zymo Research product image
ZymoPURE Plasmid Midiprep Kit
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Average Rating: 4.6
36 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2019

  • Organization: UC Irvine



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Quick and easy, highly recommend.
Rating: 4.7

  • Application Area: Routine plasmid prep

"This is the fastest plasmid purification prep kit I have ever used; even without the vacuum manifold. The price is identical to more well-known companies' but the convenience and speed make the switch worth it."

Review date: 27 Nov 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Loyola University Chicago



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great product, makes DNA preparation faster and easier.
Rating: 5.0

  • Application Area:Molecular biology, DNA preparation

"This was a very user-friendly kit. The DNA yield was great, very comparable to previous kits we've used, except cheaper."

Review date: 27 Nov 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Maryland



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Overall good. But definitely needs improvement.
Rating: 3.7

  • Application Area:Antibody and vaccine development

"It's easy to use, fast and convenient. The plasmid yield is higher than the "Q" brand. However the product seems to have some quality control issues. There is probably 50% of chance that the buffer may leak from the connection between the adapter and filter column. The yield also may vary due to the bad QC of filter columns. We tried to raise these issues to the manufacturer, but the tech support always blamed us for our not following the manual and variations among different samples. We did follow the manual. We even tested the columns by splitting one culture and passing it through two columns in the same batch; they still gave very different yields. Not to mention the leak."

Review date: 27 Nov 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: The University of Arizona



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great result!
Rating: 5.0

  • Application Area:Extract plasmids

"This is the best midiprep that I have tried. This kit gives a high amount of clean plasmid. Those clean plasmids have very nice performance in protoplast transfection. I achieved high quality and reproducible results. I will keep using it and highly recommend it."

Review date: 27 Nov 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Aptevo



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Fast kit with good DNA yields.
Rating: 4.0

  • Application Area:Purification of phagemid from E. coli strains

"This kit has been working great for me in terms of yield and quality of the phagemid DNA. It is fast and easy to use. My only comment is that sometimes it takes a long time to process the samples on the vacuum manifold. In a couple cases, the columns clogged or were very slow to clear, but they did eventually and I still had a good yield for DNA."

Review date: 27 Nov 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Texas A&M University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
High yields with high purity!
Rating: 5.0

  • Application Area:Isolation of plasmids for transient expression assays

"I used this kit for extracting plasmid for transient expression in protoplasts. It yielded up to 250 ug of plasmid DNA from high copy plasmids while ~250-1000 ng for low copy plasmids. Resulting plasmids had high purity even without the use of optional filter."

Review date: 27 Nov 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Rutgers university



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
High concentration and high purity.
Rating: 5.0

  • Application Area:Plasmid extraction

"Always get high quality plasmid DNA. It is reproducible, and easy to use."

Review date: 27 Nov 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Zoetis



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Fantastic.
Rating: 5.0

  • Application Area:Plasmid midiprep

"Fantastic."

Review date: 10 Nov 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Ruhr-Universität Bochum



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Reproducible yields of molecular biology grade plasmid.
Rating: 5.0

  • Application Area:DNA plasmid preparation

"This kit is reliable and give excellent yield of pure plasmid DNA (good 260/280 ratios). Highly purified plasmid DNA can be used for transfection, ligation, transformation."

Review date: 06 Jun 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Saarland University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great yield, simple method.
Rating: 5.0

  • Application Area:In vitro gene expression

"The yield of the purified DNA is higher compared to kits from other manufacturers. The kit is very easy to use."

Review date: 05 Jun 2019 | ZymoPURE™ II Plasmid Midiprep Kit


  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Chinta



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results. Would recommend this product for sure.
Rating: 5.0

  • Application Area:Elute DNA from the bacterial cells

"It is a really good product to use. It has given good results with great concentrations of DNA extracted."

Review date: 05 Jun 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: City of Hope



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Great kit!
Rating: 4.0

  • Application Area:Nucleic acid purification

"Quick plasmid purification kit with consistent high yield and purity."

Review date: 05 Jun 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Columbia University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
This kit is easy to use and yields high quality results.
Rating: 5.0

  • Application Area:Plasmid preparation

"The plasmid midi prep kit is extremely easy to use and consistently yields a large quantity of high-quality DNA. The Zymo vacuum manifold makes the process very quick and simple, eliminating long centrifuge steps. From start to finish, plasmids can be ready to use in less than an hour. I would highly recommend this kit for consistent, high-quality midi prep results."

Review date: 05 Jun 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Nilogen Oncosystems



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Reliable product.
Rating: 5.0

  • Application Area:Cloning of plasmids

"I used to use ZymoPURE kits for plasmid isolation while preparing recombinant plasmids for transient transfection in primary cell cultures. This kit was easy to use and always gave reliably good quality of plasmid. I never had any issue with this kit."

Review date: 05 Jun 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Delaware



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
High concentration and purified DNA.
Rating: 5.0

  • Application Area:Preparing DNA for mammalian cell transfection

"The product is extremely straightforward to use, with simple to follow instructions and clear color changes to indicate the completion of each step. The DNA yield is always high (several ug/nL with an elution volume of 75 nL) with great purity. My cells have never suffered from endotoxins as a result of DNA prepared from this kit. The vacuum manifold greatly increases throughput and efficiency of use."

Review date: 05 Jun 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Johns Hopkins University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Continue to buy it.
Rating: 4.7

  • Application Area:Transfection

"It is very fast so that I could prepare a high quality of plasmid in 40 minutes. Surpringly, it gives us tons of high copy plasmid up to 500 ug from 50 ml culture."

Review date: 05 Jun 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: SUNY-UB



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Kit costs less and is faster than previously used kit in the lab.
Rating: 5.0

  • Application Area:DNA extraction from bacterial cells

"Kit is much faster and cheaper than previous company's version that we were using. We are currently using the centrifuge method, so it could be faster still. DNA purity can be made Endo-free by performing one extra step (column is provided). Easy to use too!"

Review date: 05 Jun 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Rosalind Franklin University of Medicine & Science



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
A solid kit for extracting plasmids.
Rating: 5.0

  • Application Area:Plasmid isolation

"This kit consistently gives me a high yield and purity. The one time I was having problems extracting a particular plasmid they give me helpful tips to successfully recover it. Not so much this kit but the Zyppy midi prep was giving me strange results and they exchanged the kit because it was within their warranty."

Review date: 05 Jun 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University Of Georgia



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Great for consistent results.
Rating: 4.3

  • Application Area:Purify and collect plasmid DNA from bacterial cells

"Using ZymoPURE II Plasmid Midiprep Kit we are able to consistently achieve high plasmid DNA yields and high purity. Our downstream applications for the plasmid DNA include molecular cloning, Sanger sequencing, and transfection of mammalian cell lines."

Review date: 30 May 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Broad Institute



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
I love it! You can isolate endotoxin-free plasmid from midi culture within 20 min!
Rating: 4.3

  • Application Area:Plasmid midiprep

"Vaccum compatible High yield No need of ethanol precipitation after elution. Reasonable price."

Review date: 18 Feb 2019 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UCSF



  • Ease of use
    3 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Ok Ok
Rating: 3.7

  • Application Area:Molecular Biology

"The Zymo Midiprep kit is the only one I have used for this application, and didn't find it very convenient. It took a long time and the yields weren't great either."

Review date: 24 Aug 2016 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Population council



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Reliable product.
Rating: 5.0

  • Application Area:Cloning of specific genes

"The kit is very easy to use and it helps to save a lot of time."

Review date: 28 Jul 2016 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UCSD



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
If they improved the product, we will try it again.
Rating: 3.3

  • Application Area:Transfection

"This product's quality is not stable, sometimes works, some time has very low yields."

Review date: 27 Jul 2016 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: 5214 MS1, Univversity of Michigan



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
There is still room for improvement.
Rating: 3.7

  • Application Area:Plasmid midiprep.

"High quality DNA and high concentration of DNA for transfection. However, it is not always that you can get high yield of plasmid DNA. Success rate is about 50%."

Review date: 26 Jul 2016 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: MIT



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Unreliable.
Rating: 3.7

  • Application Area:DNA purification for transfection.

"Sometimes the kits work great (2000 ng/µL). Other times no DNA will elute from the column (30 ng/µL yield at best). This is the case for preps split in half and run on two ostensibly identical columns."

Review date: 22 Jul 2016 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Zürich



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great kit
Rating: 5.0

  • Application Area:Isolate plasmids for cloning

"Easy to use, fast and we get pure plasmids with a high yield. Works perfectly together with our strains."

Review date: 21 Jul 2016 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Houston Methodist Research Institute



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
Great results, definitely buy it again
Rating: 4.3

  • Application Area:Bacterial plasmid purification

"High quality purification and efficacy. Not time consuming and ease of handling."

Review date: 21 Jul 2016 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Can't live without it
Rating: 5.0

  • Application Area:Neuroscience

"Very easy and fast. High yield of high quality. It is faster than any other brand I ever used."

Review date: 21 Jul 2016 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Children's Hospital Los Angeles



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great product.
Rating: 5.0

  • Application Area:Molecular biology.

"Effective, easy to use, high quality and great value."

Review date: 20 Jul 2016 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Bay Area Disruptor and Startup Support Labs



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Big fan, the only kit we use for big DNA Preps!
Rating: 5.0

  • Application Area:Preparation of plasmid DNA.

"Great kit for producing very clean, high quality DNA. The yield is so high that I typically use this, instead of a Maxi-prep. Fast, easy, minimal hands on time, what more could you want? From start to finish, it typically takes less than 30 min. I haven't gotten it down to 18 min like the advertisement, but our in house vacuum is pretty weak, so the column loading is slower than average."

Review date: 18 Jul 2016 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Arkansas



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
Great results!
Rating: 4.3

  • Application Area:Cloning purpose

"Great kit. Gives good quality plasmid with ease and really fast. Highly recommended. We haven’t thought of using any other kit "

Review date: 13 Jul 2016 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Advanced Reviewer

  • Member since: 2016

  • Organization: Trinity College of Dublin



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
Best midi prep kit I used, than high name competitors like Q.
Rating: 4.3

  • Application Area:Plasmid obtention for transfection

"This kit is really better than one from a great competitor (Q), which even more cited in publications, is giving worse results in transfection. The midi prep is really easy to do, you don't need additional instruments, other than the ones used in routine mol biol labs. However, after sales care is medium, as I didn't get a reply to one of my questions. But, is worth value to get this kit rather to other for transfection in drosophila lines!!"

Review date: 12 Jul 2016 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: St. Jude



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Very happy with reliability
Rating: 5.0

  • Application Area:molecular biology

"Product is easy to use, very reliable, and affordable. We've switched to all zymo kits in our lab."

Review date: 12 Jul 2016 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Vermont



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great kit, very easy to use and significantly cheaper than many competitors
Rating: 4.7

  • Application Area:DNA purification

"Works as well as competitor with less effort (time) and reduced cost."

Review date: 12 Jul 2016 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Dana Farber Cancer Institute



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
Fast, saves a lot of time!
Rating: 4.3

  • Application Area:Plasmid DNA extraction

"Saves time in getting plasmid DNA compared to QIAGEN midiprep kits. Would be great if it works with bacteria grown in tb though. "

Review date: 12 Jul 2016 | ZymoPURE™ II Plasmid Midiprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Franklin and Marshall College



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
great results at a good price point
Rating: 4.7

  • Application Area:DNA preparation

"Routinely get microgram/mL quantities from 50mL cultures in less than an hour!"

Review date: 12 Jul 2016 | ZymoPURE™ II Plasmid Midiprep Kit

ZymoPURE ™ Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA from E. coli. The ZymoPURE ™ Plasmid Midiprep kit features a spin column-based method for the purification of up to 300 µg of high-quality plasmid DNA in less than 18 minutes. The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps.

ZymoPURE ™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl) directly from a spin column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA resulting in plasmid DNA suitable for sensitive applications including transfection, plasmid-mediated gene silencing, cloning, and sequencing.

As an added convenience, the ZymoPURE ™ Plasmid Midiprep contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Syringe filters are included for rapid clearing of the lysate and the unique spin-column design allows the binding step to be performed using a vacuum or table top centrifuge.

*Patent Pending