ZymoPURE™ II Plasmid Midiprep Kit by Zymo Research

"I am in my 4th year of PhD and still use the Midi and Mini kits for my pulldown experiments. I must say, these kits have made my life easy, also I obtain excellent quality of plasmid amount."

"Ease of use, great DNA yields, high quality DNA for transfection."

"Easy to use. produce high quality and quantity (Conc/uL) plasmid."

"Quick and easy to use. Colored buffers are fun and helpful."

"This midi-prep kit is great! It is much more efficient than other products on the market, and the colors of the reagents help ensure that you haven't made any careless mistakes when doing your preps. Highly recommend!"

"Efficient, time saving mini and maxi prep kits!"

"Quick and efficient plasmid prep kit."

"After a pretty lengthy search for a cost-effective DNA preparation solution for our lab, where we commonly transfect 30-40 mL volumes of Expi293 cells for IgG expression, I landed upon the Zymo Research ZymoPURE II Plasmid Midiprep Kit. In terms of cost, ease of use, and performance it can not be matched by any other mainstream manufacturer. The only downside that we've witnessed at this point in time is that there have been some backorder/supply delays with the product itself."

"The product is easy to use and has simple, straight forward instructions. I have used this product several times with excellent, reproducible results. The yield is always highly efficient and has good quality."

"This product is super easy to use and gives consistent results. Even our undergraduate students are able to follow the kit without any difficulties."

"I have achieved really good quality and quantity of plasmid DNA. Easy to use and rapid."

"I found this kit is relatively simple so I could save more time compared to other companies' products. After I use the sample kit, Zymo offered me a discount but I had already purchased it so I was not able to use it. Hope you can use this opportunity to get the discounts."

"The delivery was pretty fast and the product was not hard to use. Also, it didn’t require the additional steps which was what we used to do with the same kit from a different brand, which made use more convenient."

"This kit saves me time and energy knowing that every prep is going to be high yield and high quality, with zero contaminates. Most other kits leave room for a high degree of user error, where this kit is easy to use and I especially like the syringe filter system for avoiding cell debris."

"I am delighted with this midiprep kit. I used to use kits from other vendors, and I wasn't so happy. Qiagen's maxi prep kit is based on EtOH pellet, and it often gave low yield or inconsistent yield. Promega's Wizard midiprep kit is based on column technology and has a consistently good yield. However, the column wash steps take ~2hr, and the entire prep takes a half day. The Zymo kit nails both a good yield and shorter operation time. I can also advocate for its high quality, as I gained higher transformation efficiency from plasmids extracted in this kit. Lastly, the price is comparable or even cheaper than the competitors. The only complaint I have is that it comes with two large boxes for 25 preps, and it takes up larger shelf space on my bench."

"This kit is very friendly for researchers, especially for a fresh guy. The best thing is it contains color buffers. The solution will turn the indicated color after you add buffer P1, P2 and P3. It prevents mistakes or missing buffers. I get high quality and reproducible plasmids every time I use the kit."

"High quality reproducible results. I get good spectra and high yields each time, the product is easy to use and is quick. The one problem I have with it is the yield loss with the EndoZero columns. It tends to be enough that we have stopped using them altogether. Luckily our preps are clean enough for neural transfection without it."

"Very easy to use DNA isolation kit, this is far more efficient than other similar products we have tried. Because all of the solvents are colored differently, it helps you prevent making significant mistakes in sample preparation."

"Very easy and "user friendly" product! I had a great plasmid yield with minimum effort and in a timely manner. Highly recommend!"

"I like it in general because it gives excellent yield. However, during preparation the Zymo-Spin™ III-P column assemblies do not provide the best seal to the vacuum pump, that slows down the midiprep significantly."

"We’ve made good quality plasmids using this purification kit."

"This plasmid prep kit is so easy to use and yields good quality DNA. The colored buffers make it easy to keep track of the correct solution for the correct step. It’s so quick and I always get great yield. We have switched to this and many zymo kits fornroutine use in our lab."

"The concentration of extracted plasmid DNA from a 50 ml culture can reach 1500-3500 ng/uL in a 400 uL elution. It is of high quality and low endotoxin level (there is a column for it). Very satisfied with this product!"

"This midiprep kit produces a higher yield in microgram per microliters. It is very useful in experiments that demand a higher amount of DNA"

"I used this kit on six samples. It gave me the highest yield for DNA I've ever had. I had 4ug of DNA from each sample in 100uL. The protocol and instruction were very easy, and the video they have for the product makes it even easier. It’s actually fun to use because it makes things very simple. It is also worth mentioning that the kit's price is cheaper than any other midiprep kits online."

"As with all the Zymo products, this product is a great value. The procedure itself is quick and easy; the color-changing buffers are definitely a plus when it comes to making sure your suspension is processed properly. I used the alternative centrifugation method to process our sample and had zero issues. Recovery was fantastic."

"This is the fastest plasmid purification prep kit I have ever used; even without the vacuum manifold. The price is identical to more well-known companies' but the convenience and speed make the switch worth it."

"This was a very user-friendly kit. The DNA yield was great, very comparable to previous kits we've used, except cheaper."

"It's easy to use, fast and convenient. The plasmid yield is higher than the "Q" brand. However the product seems to have some quality control issues. There is probably 50% of chance that the buffer may leak from the connection between the adapter and filter column. The yield also may vary due to the bad QC of filter columns. We tried to raise these issues to the manufacturer, but the tech support always blamed us for our not following the manual and variations among different samples. We did follow the manual. We even tested the columns by splitting one culture and passing it through two columns in the same batch; they still gave very different yields. Not to mention the leak."

"This is the best midiprep that I have tried. This kit gives a high amount of clean plasmid. Those clean plasmids have very nice performance in protoplast transfection. I achieved high quality and reproducible results. I will keep using it and highly recommend it."

"This kit has been working great for me in terms of yield and quality of the phagemid DNA. It is fast and easy to use. My only comment is that sometimes it takes a long time to process the samples on the vacuum manifold. In a couple cases, the columns clogged or were very slow to clear, but they did eventually and I still had a good yield for DNA."

"I used this kit for extracting plasmid for transient expression in protoplasts. It yielded up to 250 ug of plasmid DNA from high copy plasmids while ~250-1000 ng for low copy plasmids. Resulting plasmids had high purity even without the use of optional filter."

"Always get high quality plasmid DNA. It is reproducible, and easy to use."

"Fantastic."

"This kit is reliable and give excellent yield of pure plasmid DNA (good 260/280 ratios). Highly purified plasmid DNA can be used for transfection, ligation, transformation."

"The yield of the purified DNA is higher compared to kits from other manufacturers. The kit is very easy to use."

"It is a really good product to use. It has given good results with great concentrations of DNA extracted."

"Quick plasmid purification kit with consistent high yield and purity."

"The plasmid midi prep kit is extremely easy to use and consistently yields a large quantity of high-quality DNA. The Zymo vacuum manifold makes the process very quick and simple, eliminating long centrifuge steps. From start to finish, plasmids can be ready to use in less than an hour. I would highly recommend this kit for consistent, high-quality midi prep results."

"I used to use ZymoPURE kits for plasmid isolation while preparing recombinant plasmids for transient transfection in primary cell cultures. This kit was easy to use and always gave reliably good quality of plasmid. I never had any issue with this kit."

"The product is extremely straightforward to use, with simple to follow instructions and clear color changes to indicate the completion of each step. The DNA yield is always high (several ug/nL with an elution volume of 75 nL) with great purity. My cells have never suffered from endotoxins as a result of DNA prepared from this kit. The vacuum manifold greatly increases throughput and efficiency of use."

"It is very fast so that I could prepare a high quality of plasmid in 40 minutes. Surpringly, it gives us tons of high copy plasmid up to 500 ug from 50 ml culture."

"Kit is much faster and cheaper than previous company's version that we were using. We are currently using the centrifuge method, so it could be faster still. DNA purity can be made Endo-free by performing one extra step (column is provided). Easy to use too!"

"This kit consistently gives me a high yield and purity. The one time I was having problems extracting a particular plasmid they give me helpful tips to successfully recover it. Not so much this kit but the Zyppy midi prep was giving me strange results and they exchanged the kit because it was within their warranty."

"Using ZymoPURE II Plasmid Midiprep Kit we are able to consistently achieve high plasmid DNA yields and high purity. Our downstream applications for the plasmid DNA include molecular cloning, Sanger sequencing, and transfection of mammalian cell lines."

"Vaccum compatible High yield No need of ethanol precipitation after elution. Reasonable price."

"The Zymo Midiprep kit is the only one I have used for this application, and didn't find it very convenient. It took a long time and the yields weren't great either."

"The kit is very easy to use and it helps to save a lot of time."

"This product's quality is not stable, sometimes works, some time has very low yields."

"High quality DNA and high concentration of DNA for transfection. However, it is not always that you can get high yield of plasmid DNA. Success rate is about 50%."

"Sometimes the kits work great (2000 ng/µL). Other times no DNA will elute from the column (30 ng/µL yield at best). This is the case for preps split in half and run on two ostensibly identical columns."

"Easy to use, fast and we get pure plasmids with a high yield. Works perfectly together with our strains."

"High quality purification and efficacy. Not time consuming and ease of handling."

"Very easy and fast. High yield of high quality. It is faster than any other brand I ever used."

"Effective, easy to use, high quality and great value."

"Great kit for producing very clean, high quality DNA. The yield is so high that I typically use this, instead of a Maxi-prep. Fast, easy, minimal hands on time, what more could you want? From start to finish, it typically takes less than 30 min. I haven't gotten it down to 18 min like the advertisement, but our in house vacuum is pretty weak, so the column loading is slower than average."

"Great kit. Gives good quality plasmid with ease and really fast. Highly recommended. We haven’t thought of using any other kit "

"This kit is really better than one from a great competitor (Q), which even more cited in publications, is giving worse results in transfection. The midi prep is really easy to do, you don't need additional instruments, other than the ones used in routine mol biol labs. However, after sales care is medium, as I didn't get a reply to one of my questions. But, is worth value to get this kit rather to other for transfection in drosophila lines!!"

"Product is easy to use, very reliable, and affordable. We've switched to all zymo kits in our lab."

"Works as well as competitor with less effort (time) and reduced cost."

"Saves time in getting plasmid DNA compared to QIAGEN midiprep kits. Would be great if it works with bacteria grown in tb though. "

"Routinely get microgram/mL quantities from 50mL cultures in less than an hour!"