ZymoPURE™ II Plasmid Maxiprep Kit
The ZymoPURE™ Plasmid Maxiprep kit features a spin column-based method for the purification of up to 1.2 mg of high-quality plasmid DNA in less than 20 minutes.
ZymoPURE Plasmid Maxiprep Kit
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The kit is easy, fast and I had an excelent yield
Plasmid extraction
I work with a low copy number plasmid and ZymoPURE II Plasmid Maxiprep Kit solved my problems The manipulation was very intuitive and my extraction results were above expectation
Review Date: 9 Feb 2023 | Zymo Research
Great yield, fast prep time
Crispr cas plasmid prep
High quality plasmid with robust transfection efficiency
Review Date: 15 Dec 2022 | Zymo Research
Great result, easy to use
Transfection
Easy to use, produced a high yield of DNA of the good quality, and was cost-effective
Review Date: 14 Dec 2022 | Zymo Research
User friendly and easy to use
Plasmid purification
Plasmid prep or purification kits are user friendly and easy to use
Review Date: 7 Dec 2022 | Zymo Research
Great results for a lower price than competitors.
plasmid preparation for psuedovirus production
The product is as efficient as competitor's products while being more affordable.
Review Date: 6 Dec 2022 | Zymo Research
Very easy to use
Extraction of plasmids from bacteria culture
I would say it is easy to use and straight to the point. I would rate the yield to be about 60%, purity at 80%. It is good value for the money nonetheless.
Review Date: 6 Dec 2022 | Zymo Research
Very quick and excellent quality of DNA
Preparation of DNA plasmid for mammalian cell transfection
We have been using this kit for a long time. Once E. coli cells are resuspended, it takes only 30 min or so. As a final step, the residual amount of endotoxin can be removed by a simple spin. We used to use another company's products, but this kit from Zymoresearch saves our time and money.
Review Date: 6 Dec 2022 | Zymo Research
Great and efficient kit
Plasmid Prep from Glycerol Stocks
I like how fast and efficient this kit it. Our lab used to use a kit that took 3 hours for a maxiprep preparation and required several centrifugation steps. The Zymo Kit allows me to do the same thing in 45 minutes with very comparable yields. I would definitely recommend it when you don't want to spend one day doing Maxiprep and also to save money.
Review Date: 6 Dec 2022 | Zymo Research
Inexpensive, fast, and easy
High yield purification of bacterial plasmids
The kit is by far the fastest maxikit I've used while not sacrificing yield. You'll have a hard time finding a comparable endotoxin free maxikit in this price range. I got yields of 2~3 ug/uL, ~300 uL total, but it varies depending on the plasmid itself and how you grow the bacteria. The included manual is very clear and in-depth. The kit shipped promptly.
Review Date: 23 Nov 2022 | Zymo Research
Great results!
Plasmids preps
I used a maxiprep kit from another vendor for many years but decided to test ZymoPURE II since it was cheaper and my experience with Zymo kits. I was impacted by how easy is to use ZymoPURE II to have high quality and amount of plasmid for transfections in less than an hour. Definitely I recommend it.
Review Date: 21 Nov 2022 | Zymo Research
ZymoPURE™ Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA from E. coli. The ZymoPURE™ Plasmid Maxiprep kit features a spin column-based method for the purification of up to 1.2 mg of high-quality plasmid DNA in less than 18 minutes. The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps.
ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl) directly from a spin column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA resulting in plasmid DNA suitable for sensitive applications including transfection, plasmid-mediated gene silencing, cloning, and sequencing.
As an added convenience, the ZymoPURE™ Plasmid Maxiprep contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Syringe filters are included for rapid clearing of the lysate and the unique spin-column design allows the binding step to be performed using a vacuum or table top centrifuge.
*Patent Pending
Maximizing transfection efficiency: The importance of plasmid DNA quality
In this application note, Zymo Research emphasizes the significant impact of plasmid DNA quality on transfection success. Four key points are highlighted:
- The DNA should be predominantly supercoiled and free of genomic DNA and RNA, as circular plasmids are more efficiently taken up by cells, while contamination confuses quantification.
- The DNA should be highly concentrated and devoid of phenol, salts, and protein to prevent dilution of transfection reagents, skewed absorbance readings, and inhibition of DNA-lipid complex formation.
- It is crucial to ensure the DNA is free of endotoxins, as these impurities reduce transfection efficiency and harm sensitive cells.
- Confirming the correct sequence of the plasmid is vital for achieving experimental objectives and serves as a good assessment of its purity and suitability for transfections. Maintaining a good absorbance spectrum is essential for optimal results.
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Direct-lysis, miniprep, and ultra-endotoxin-free versions of ZymoPURE to be released
