ZymoPURE™ II Plasmid Gigaprep Kit by Zymo Research

Manufacturer Zymo Research  |   Model: D4204
4.3
/
5.0
  |  4 reviews
The fastest spin-column based procedure for purifying up to 10 mg of ultra-pure endotoxin-free transfection-grade plasmid DNA.


ZymoPURE™ II Plasmid Gigaprep Kit by Zymo Research product image
ZymoPURE Plasmid Gigaprep Kit

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Average Rating: 4.3
4 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2019

  • Organization: Precision Bio Laboratories Inc



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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One of the best kits out in the market
Rating: 4.7

  • Application Area: Purification of plasmids

"Great kit, easy work flow, good DNA quality, and considerably good yield. Worth every dime you spend."

Review date: 11 Dec 2019 | ZymoPURE™ II Plasmid Gigaprep Kit
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: University of Florida



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product that saves the lab time and money
Rating: 5.0

  • Application Area:Gene therapy vector construction

"On average, we were able to get about 3x the DNA compared to Qiagen Giga kits. After the endotoxin issues were worked out of the early product line, this product has proven itself to our satisfaction. We are also able to prepare our plasmid DNA in about half the time."

Review date: 02 Dec 2019 | ZymoPURE™ II Plasmid Gigaprep Kit
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: NNF Center for Protein Research



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Fast, reliable, high yield.
Rating: 4.3

  • Application Area:Plasmid production for transfection purposes

"Very high yield of DNA is achieved, even for big/difficult constructs. I really appreciated that the protocol is fast (especially the fact that there's no isopropanol precipitation at the end), reliable and easy. I like the idea of the coloured buffers."

Review date: 05 Jun 2019 | ZymoPURE™ II Plasmid Gigaprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: AbbVie Inc.



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
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Did not achieve DNA yield as stated by Zymo research.
Rating: 3.3

  • Application Area:DNA purification.

"The steps were easy to follow. It requires more than the ZymoPURE™ Maxiprep Kits in terms of buffer and centrifuge use. This Gigaprep Kit however, yielded far less than what Zymo Research claims. I get better yields with the ZymoPURE™ Maxiprep Kits."

Review date: 26 Jul 2016 | ZymoPURE™ II Plasmid Gigaprep Kit

ZymoPURE Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA from E. coli. The ZymoPURE Plasmid Gigaprep kit features a spin column-based method for the purification of up to 10 mg of high-quality plasmid DNA in less than 50 minutes. The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps.

ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl) directly from a spin column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA resulting in plasmid DNA suitable for sensitive applications including transfection, plasmid-mediated gene silencing, cloning, and sequencing.

As an added convenience, the ZymoPURE Plasmid Gigaprep contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Also, bottle top filters are included for rapid clearing of the lysate.

*Patent Pending

Product Overview

ZymoPURE™ II Plasmid Gigaprep Kit by Zymo Research product image

ZymoPURE™ II Plasmid Gigaprep Kit

Manufacturer Zymo Research

4.3 / 5.0 | 4 reviews