Status:
ReviewerMember since: 2022
Organization: Unlocked Labs
Easy to use and highly efficient
Application Area: Purify plasmid from large culture
"I have used it several times for overnight cultures of 50mL. Each time I obtained high yields with little hassle with the kit. It is easy to use and worth the money."
Status:
ReviewerMember since: 2022
Organization: Altus Enterprises, Inc
Excellent product, easy to tailor to in house use, <3 hours for 8 samples!
Application Area:Plasmid purification for mutagenesis and transfection
"Cell Source: Thermo TOP10 Cells Culture Volume: 100mL Process Time: 8 samples takes ~2-3 hours from before culture spin down to completion of elution The protocol says 50mL of culture but we have adapted it for use with 100mL. The column say that they can hold up to 1.2mg of DNA and this culture volume works for us. This protocol is very resilient to user error and is consistent enough that we have had new interns reaching competency very quickly on this protocol. We have been using this product for over 1 year and the failure rate (<100ug) is <5%."
Status:
ReviewerMember since: 2022
Organization: Agathos Biologics
Easy to use & adjust to experiments
Application Area:Bacmid DNA Purification
"This Zymo kit is a breeze to use- the instructions are clear, visual, and detailed, and the color-coded reagents make it easy to identify at a glance what is what. The kit is also readily adapted to the specifics of the experiment. If increasing the DNA yield or removing endotoxins is important, there are optional steps to include in your procedure. Also, replacing individual kit components does not require you to buy a whole new kit- every component has a part number that allows you to buy it directly from the manufacturer's website. Would highly recommend this product!"
Status:
ReviewerMember since: 2022
Organization: UTSW
Super easy for my first time doing a midi prep
Application Area:Using the midi prep to expand DNA
"I can't say better things about this kit. it was simple, streamline and came quick. I had no problems and had great results every time! I will never use another product!"
Status:
ReviewerMember since: 2022
Organization: McNeil
Good results, great price
Application Area:Plasmid purification for cell line transfection
"This worked as well as Qiagen's kit does, and at a fraction of the cost. I'm not going back to Qiagen, I'll tell you that much!"
Status:
ReviewerMember since: 2022
Organization: JHMI
This is the product we have been using for years.
Application Area:Plasmid purification
"Easy to use and high yields. Has the option to remove the endotoxin."
Status:
ReviewerMember since: 2022
Organization: University of California Berkeley
Fabulous!
Application Area:Molecular biology
"The Zymo midi/maxi kits are great! It would be even better if the syringe filters/columns could fit into 50 mL conicals."
Status:
ReviewerMember since: 2021
Organization: CSIR-Indian Institute of Chemical Technology
Great product.
Application Area:Plasmid for transfection
"The sales team is very good and the product is wonderful."
Status:
ReviewerMember since: 2021
Organization: University of Nevada, Las Vegas
Absolutely love this kit! Never using anything else
Application Area:Plasmid Extraction
"When I first started my Ph.D., I had to do a lot of plasmid extractions. I struggled for months using different kits. I convinced my PI to purchase the Zymo Midiprep kit and I have successfully extracted high-quality plasmids without fail. Now, I can finally move on with my research and not wonder if I will get a high yield of high-quality plasmid. One of my favorite things about this kit is that the buffers are different colors. This is especially helpful for the neutralization step which allowed me to be confident in moving on to the next step. Another thing that I love about this kit is that the elution volume is 200uL. Other kits elute 1mL from the column which means that you need to precipitate the DNA in order to get a high concentration. This was especially important for me since I regularly needed 1ug/uL of plasmid DNA. I love everything about this kit and it is very quick to use. One of the best things is that it's cheap and of VERY good quality! Now, my lab has transitioned to using Zymo for Miniprep, Midiprep, and Maxiprep! Thank you, Zymo!!!"
Status:
ReviewerMember since: 2021
Organization: CSIR-IICT HYDERABAD
Excellent results. Still be using and recommending others.
Application Area:To obtain transfection grade plasmids for Coimmuniprecipitation
"I am in my 4th year of PhD and still use the Midi and Mini kits for my pulldown experiments. I must say, these kits have made my life easy, also I obtain excellent quality of plasmid amount."
Status:
ReviewerMember since: 2021
Organization: Altus Enterprises Inc.
Keep up the good work.
Application Area:DNA prep for transfection
"Ease of use, great DNA yields, high quality DNA for transfection."
Status:
ReviewerMember since: 2021
Organization: Baylor College of Medicine
Great results. High conc. Quality product.
Application Area:Isolation of plasmid
"Easy to use. produce high quality and quantity (Conc/uL) plasmid."
Status:
ReviewerMember since: 2021
Organization: UCSC
Highly recommended for all your plasmid prep needs.
Application Area:Plasmid purification
"Quick and easy to use. Colored buffers are fun and helpful."
Status:
ReviewerMember since: 2021
Organization: Columbia University Medical Center
Amazing kit!
Application Area:Molecular biology
"This midi-prep kit is great! It is much more efficient than other products on the market, and the colors of the reagents help ensure that you haven't made any careless mistakes when doing your preps. Highly recommend!"
Status:
ReviewerMember since: 2021
Organization: Phenomic AI
Extremely easy to use, rapid protocol, high outputs, transfection-quality DNA.
Application Area:Preparing DNA for Expi293 transfection on 35 mL scale
"After a pretty lengthy search for a cost-effective DNA preparation solution for our lab, where we commonly transfect 30-40 mL volumes of Expi293 cells for IgG expression, I landed upon the Zymo Research ZymoPURE II Plasmid Midiprep Kit. In terms of cost, ease of use, and performance it can not be matched by any other mainstream manufacturer. The only downside that we've witnessed at this point in time is that there have been some backorder/supply delays with the product itself."
Status:
ReviewerMember since: 2021
Organization: Sam Houston State University
Great results, high yield of good quality plasmid DNA.
Application Area:Develop a diagnostic RT-qPCR assay
"The product is easy to use and has simple, straight forward instructions. I have used this product several times with excellent, reproducible results. The yield is always highly efficient and has good quality."
Status:
ReviewerMember since: 2021
Organization: Baylor College of medicine
Ease of use is unparalleled.
Application Area:Purify plasmid DNA from bacteria
"This product is super easy to use and gives consistent results. Even our undergraduate students are able to follow the kit without any difficulties."
Status:
ReviewerMember since: 2021
Organization: The Hormel Institute
Great results and I enjoy using Zymo products.
Application Area:Analyze cell and tissue samples
"I have achieved really good quality and quantity of plasmid DNA. Easy to use and rapid."
Status:
ReviewerMember since: 2020
Organization: Sanford Burnham Prebys Medical Discovery Institute
Great results, and fast protocols! Love it.
Application Area:Isolation of plasmid DNA
"I found this kit is relatively simple so I could save more time compared to other companies' products. After I use the sample kit, Zymo offered me a discount but I had already purchased it so I was not able to use it. Hope you can use this opportunity to get the discounts."
Status:
ReviewerMember since: 2021
Organization: BYU
Pretty consistent result for different DNA.
Application Area:Analyze the amount of the interest of DNA after cloning
"The delivery was pretty fast and the product was not hard to use. Also, it didn’t require the additional steps which was what we used to do with the same kit from a different brand, which made use more convenient."
Status:
ReviewerMember since: 2021
Organization: Cornell University
Excellent product, far easier and more efficient than all other kits.
Application Area:Plasmid engineering and sequencing
"This kit saves me time and energy knowing that every prep is going to be high yield and high quality, with zero contaminates. Most other kits leave room for a high degree of user error, where this kit is easy to use and I especially like the syringe filter system for avoiding cell debris."
Status:
ReviewerMember since: 2020
Organization: University of Pennsylvania
Consistently high yield and high quality plasmids, with the competitive price.
Application Area:Extract plasmids
"I am delighted with this midiprep kit. I used to use kits from other vendors, and I wasn't so happy. Qiagen's maxi prep kit is based on EtOH pellet, and it often gave low yield or inconsistent yield. Promega's Wizard midiprep kit is based on column technology and has a consistently good yield. However, the column wash steps take ~2hr, and the entire prep takes a half day. The Zymo kit nails both a good yield and shorter operation time. I can also advocate for its high quality, as I gained higher transformation efficiency from plasmids extracted in this kit. Lastly, the price is comparable or even cheaper than the competitors. The only complaint I have is that it comes with two large boxes for 25 preps, and it takes up larger shelf space on my bench."
Status:
ReviewerMember since: 2020
Organization: Houston Methodist Research Institute
Excellent kit! Great help to my research.
Application Area:Obtain plasmids to establish gene expression system
"This kit is very friendly for researchers, especially for a fresh guy. The best thing is it contains color buffers. The solution will turn the indicated color after you add buffer P1, P2 and P3. It prevents mistakes or missing buffers. I get high quality and reproducible plasmids every time I use the kit."
Status:
ReviewerMember since: 2020
Organization: UNC-CH
Overall great, EndoZero could use work.
Application Area:Purifying plasmids
"High quality reproducible results. I get good spectra and high yields each time, the product is easy to use and is quick. The one problem I have with it is the yield loss with the EndoZero columns. It tends to be enough that we have stopped using them altogether. Luckily our preps are clean enough for neural transfection without it."
Status:
ReviewerMember since: 2020
Organization: Columbia University Medical Center
Great midiprep product!
Application Area:Plasmid isolation
"Very easy to use DNA isolation kit, this is far more efficient than other similar products we have tried. Because all of the solvents are colored differently, it helps you prevent making significant mistakes in sample preparation."
Status:
ReviewerMember since: 2020
Organization: UCDavis
Recommended if you want to truly enjoy your plasmid purification.
Application Area:Plasmid purification for basic cancer research
"Very easy and "user friendly" product! I had a great plasmid yield with minimum effort and in a timely manner. Highly recommend!"
Status:
ReviewerMember since: 2020
Organization: Ascidian
Hope to solve the vacuum problem I encountered.
Application Area:Purify plasmids
"I like it in general because it gives excellent yield. However, during preparation the Zymo-Spin™ III-P column assemblies do not provide the best seal to the vacuum pump, that slows down the midiprep significantly."
Status:
ReviewerMember since: 2020
Organization: University of Georgia
Quality plasmids.
Application Area:Plasmid purification for mammalian cell transfection
"We’ve made good quality plasmids using this purification kit."
Status:
ReviewerMember since: 2019
Organization: University of Pittsburgh
Easy to use and great yield!
Application Area:DNA plasmid amplification
"This plasmid prep kit is so easy to use and yields good quality DNA. The colored buffers make it easy to keep track of the correct solution for the correct step. It’s so quick and I always get great yield. We have switched to this and many zymo kits fornroutine use in our lab."
Status:
ReviewerMember since: 2020
Organization: Princeton University
A very good deal!
Application Area:Extract plasmid DNA from E. coli with low endo-toxin level
"The concentration of extracted plasmid DNA from a 50 ml culture can reach 1500-3500 ng/uL in a 400 uL elution. It is of high quality and low endotoxin level (there is a column for it). Very satisfied with this product!"
Status:
ReviewerMember since: 2019
Organization: Baylor University
Great results
Application Area:Plasmid midiprep
"This midiprep kit produces a higher yield in microgram per microliters. It is very useful in experiments that demand a higher amount of DNA"
Status:
ReviewerMember since: 2019
Organization: West Virginia University
Highly recommended for your plasmid DNA midiprep
Application Area:plasmid DNA midiprep for transfection in myeloma cells
"I used this kit on six samples. It gave me the highest yield for DNA I've ever had. I had 4ug of DNA from each sample in 100uL. The protocol and instruction were very easy, and the video they have for the product makes it even easier. It’s actually fun to use because it makes things very simple. It is also worth mentioning that the kit's price is cheaper than any other midiprep kits online."
Status:
ReviewerMember since: 2019
Organization: Albany College of Pharmacy and Health Sciences
Easy and effective kit
Application Area:Isolate plasmids from E. coli
"As with all the Zymo products, this product is a great value. The procedure itself is quick and easy; the color-changing buffers are definitely a plus when it comes to making sure your suspension is processed properly. I used the alternative centrifugation method to process our sample and had zero issues. Recovery was fantastic."
Status:
ReviewerMember since: 2019
Organization: UC Irvine
Quick and easy, highly recommend.
Application Area:Routine plasmid prep
"This is the fastest plasmid purification prep kit I have ever used; even without the vacuum manifold. The price is identical to more well-known companies' but the convenience and speed make the switch worth it."
Status:
ReviewerMember since: 2019
Organization: Loyola University Chicago
Great product, makes DNA preparation faster and easier.
Application Area:Molecular biology, DNA preparation
"This was a very user-friendly kit. The DNA yield was great, very comparable to previous kits we've used, except cheaper."
Status:
ReviewerMember since: 2019
Organization: University of Maryland
Overall good. But definitely needs improvement.
Application Area:Antibody and vaccine development
"It's easy to use, fast and convenient. The plasmid yield is higher than the "Q" brand. However the product seems to have some quality control issues. There is probably 50% of chance that the buffer may leak from the connection between the adapter and filter column. The yield also may vary due to the bad QC of filter columns. We tried to raise these issues to the manufacturer, but the tech support always blamed us for our not following the manual and variations among different samples. We did follow the manual. We even tested the columns by splitting one culture and passing it through two columns in the same batch; they still gave very different yields. Not to mention the leak."
Status:
ReviewerMember since: 2010
Organization: The University of Arizona
Great result!
Application Area:Extract plasmids
"This is the best midiprep that I have tried. This kit gives a high amount of clean plasmid. Those clean plasmids have very nice performance in protoplast transfection. I achieved high quality and reproducible results. I will keep using it and highly recommend it."
Status:
ReviewerMember since: 2019
Organization: Aptevo
Fast kit with good DNA yields.
Application Area:Purification of phagemid from E. coli strains
"This kit has been working great for me in terms of yield and quality of the phagemid DNA. It is fast and easy to use. My only comment is that sometimes it takes a long time to process the samples on the vacuum manifold. In a couple cases, the columns clogged or were very slow to clear, but they did eventually and I still had a good yield for DNA."
Status:
ReviewerMember since: 2019
Organization: Texas A&M University
High yields with high purity!
Application Area:Isolation of plasmids for transient expression assays
"I used this kit for extracting plasmid for transient expression in protoplasts. It yielded up to 250 ug of plasmid DNA from high copy plasmids while ~250-1000 ng for low copy plasmids. Resulting plasmids had high purity even without the use of optional filter."
Status:
ReviewerMember since: 2019
Organization: Rutgers university
High concentration and high purity.
Application Area:Plasmid extraction
"Always get high quality plasmid DNA. It is reproducible, and easy to use."
Status:
ReviewerMember since: 2019
Organization: Ruhr-Universität Bochum
Reproducible yields of molecular biology grade plasmid.
Application Area:DNA plasmid preparation
"This kit is reliable and give excellent yield of pure plasmid DNA (good 260/280 ratios). Highly purified plasmid DNA can be used for transfection, ligation, transformation."
Status:
ReviewerMember since: 2019
Organization: Saarland University
Great yield, simple method.
Application Area:In vitro gene expression
"The yield of the purified DNA is higher compared to kits from other manufacturers. The kit is very easy to use."
Status:
ReviewerMember since: 2019
Organization: Chinta
Great results. Would recommend this product for sure.
Application Area:Elute DNA from the bacterial cells
"It is a really good product to use. It has given good results with great concentrations of DNA extracted."
Status:
ReviewerMember since: 2019
Organization: City of Hope
Great kit!
Application Area:Nucleic acid purification
"Quick plasmid purification kit with consistent high yield and purity."
Status:
ReviewerMember since: 2019
Organization: Columbia University
This kit is easy to use and yields high quality results.
Application Area:Plasmid preparation
"The plasmid midi prep kit is extremely easy to use and consistently yields a large quantity of high-quality DNA. The Zymo vacuum manifold makes the process very quick and simple, eliminating long centrifuge steps. From start to finish, plasmids can be ready to use in less than an hour. I would highly recommend this kit for consistent, high-quality midi prep results."
Status:
ReviewerMember since: 2019
Organization: Nilogen Oncosystems
Reliable product.
Application Area:Cloning of plasmids
"I used to use ZymoPURE kits for plasmid isolation while preparing recombinant plasmids for transient transfection in primary cell cultures. This kit was easy to use and always gave reliably good quality of plasmid. I never had any issue with this kit."
Status:
ReviewerMember since: 2019
Organization: University of Delaware
High concentration and purified DNA.
Application Area:Preparing DNA for mammalian cell transfection
"The product is extremely straightforward to use, with simple to follow instructions and clear color changes to indicate the completion of each step. The DNA yield is always high (several ug/nL with an elution volume of 75 nL) with great purity. My cells have never suffered from endotoxins as a result of DNA prepared from this kit. The vacuum manifold greatly increases throughput and efficiency of use."
Status:
ReviewerMember since: 2019
Organization: Johns Hopkins University
Continue to buy it.
Application Area:Transfection
"It is very fast so that I could prepare a high quality of plasmid in 40 minutes. Surpringly, it gives us tons of high copy plasmid up to 500 ug from 50 ml culture."
Status:
ReviewerMember since: 2019
Organization: SUNY-UB
Kit costs less and is faster than previously used kit in the lab.
Application Area:DNA extraction from bacterial cells
"Kit is much faster and cheaper than previous company's version that we were using. We are currently using the centrifuge method, so it could be faster still. DNA purity can be made Endo-free by performing one extra step (column is provided). Easy to use too!"
Status:
ReviewerMember since: 2019
Organization: Rosalind Franklin University of Medicine & Science
A solid kit for extracting plasmids.
Application Area:Plasmid isolation
"This kit consistently gives me a high yield and purity. The one time I was having problems extracting a particular plasmid they give me helpful tips to successfully recover it. Not so much this kit but the Zyppy midi prep was giving me strange results and they exchanged the kit because it was within their warranty."
Status:
ReviewerMember since: 2019
Organization: University Of Georgia
Great for consistent results.
Application Area:Purify and collect plasmid DNA from bacterial cells
"Using ZymoPURE II Plasmid Midiprep Kit we are able to consistently achieve high plasmid DNA yields and high purity. Our downstream applications for the plasmid DNA include molecular cloning, Sanger sequencing, and transfection of mammalian cell lines."
Status:
Advanced ReviewerMember since: 2018
Organization: Broad Institute
I love it! You can isolate endotoxin-free plasmid from midi culture within 20 min!
Application Area:Plasmid midiprep
"Vaccum compatible High yield No need of ethanol precipitation after elution. Reasonable price."
Status:
ReviewerMember since: 2016
Organization: UCSF
Ok Ok
Application Area:Molecular Biology
"The Zymo Midiprep kit is the only one I have used for this application, and didn't find it very convenient. It took a long time and the yields weren't great either."
Status:
ReviewerMember since: 2016
Organization: Population council
Reliable product.
Application Area:Cloning of specific genes
"The kit is very easy to use and it helps to save a lot of time."
Status:
ReviewerMember since: 2016
Organization: UCSD
If they improved the product, we will try it again.
Application Area:Transfection
"This product's quality is not stable, sometimes works, some time has very low yields."
Status:
ReviewerMember since: 2016
Organization: 5214 MS1, Univversity of Michigan
There is still room for improvement.
Application Area:Plasmid midiprep.
"High quality DNA and high concentration of DNA for transfection. However, it is not always that you can get high yield of plasmid DNA. Success rate is about 50%."
Status:
ReviewerMember since: 2016
Organization: MIT
Unreliable.
Application Area:DNA purification for transfection.
"Sometimes the kits work great (2000 ng/µL). Other times no DNA will elute from the column (30 ng/µL yield at best). This is the case for preps split in half and run on two ostensibly identical columns."
Status:
ReviewerMember since: 2016
Organization: University of Zürich
Great kit
Application Area:Isolate plasmids for cloning
"Easy to use, fast and we get pure plasmids with a high yield. Works perfectly together with our strains."
Status:
ReviewerMember since: 2016
Organization: Houston Methodist Research Institute
Great results, definitely buy it again
Application Area:Bacterial plasmid purification
"High quality purification and efficacy. Not time consuming and ease of handling."
Status:
ReviewerMember since: 2016
Organization: University
Can't live without it
Application Area:Neuroscience
"Very easy and fast. High yield of high quality. It is faster than any other brand I ever used."
Status:
ReviewerMember since: 2016
Organization: Children's Hospital Los Angeles
Great product.
Application Area:Molecular biology.
"Effective, easy to use, high quality and great value."
Status:
ReviewerMember since: 2016
Organization: Bay Area Disruptor and Startup Support Labs
Big fan, the only kit we use for big DNA Preps!
Application Area:Preparation of plasmid DNA.
"Great kit for producing very clean, high quality DNA. The yield is so high that I typically use this, instead of a Maxi-prep. Fast, easy, minimal hands on time, what more could you want? From start to finish, it typically takes less than 30 min. I haven't gotten it down to 18 min like the advertisement, but our in house vacuum is pretty weak, so the column loading is slower than average."
Status:
ReviewerMember since: 2016
Organization: University of Arkansas
Great results!
Application Area:Cloning purpose
"Great kit. Gives good quality plasmid with ease and really fast. Highly recommended. We haven’t thought of using any other kit "
Status:
Advanced ReviewerMember since: 2016
Organization: Trinity College of Dublin
Best midi prep kit I used, than high name competitors like Q.
Application Area:Plasmid obtention for transfection
"This kit is really better than one from a great competitor (Q), which even more cited in publications, is giving worse results in transfection. The midi prep is really easy to do, you don't need additional instruments, other than the ones used in routine mol biol labs. However, after sales care is medium, as I didn't get a reply to one of my questions. But, is worth value to get this kit rather to other for transfection in drosophila lines!!"
Status:
ReviewerMember since: 2016
Organization: St. Jude
Very happy with reliability
Application Area:molecular biology
"Product is easy to use, very reliable, and affordable. We've switched to all zymo kits in our lab."
Status:
ReviewerMember since: 2016
Organization: University of Vermont
Great kit, very easy to use and significantly cheaper than many competitors
Application Area:DNA purification
"Works as well as competitor with less effort (time) and reduced cost."
Status:
ReviewerMember since: 2016
Organization: Dana Farber Cancer Institute
Fast, saves a lot of time!
Application Area:Plasmid DNA extraction
"Saves time in getting plasmid DNA compared to QIAGEN midiprep kits. Would be great if it works with bacteria grown in tb though. "
Status:
ReviewerMember since: 2016
Organization: Franklin and Marshall College
great results at a good price point
Application Area:DNA preparation
"Routinely get microgram/mL quantities from 50mL cultures in less than an hour!"
ZymoPURE ™ Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA from E. coli. The ZymoPURE ™ Plasmid Midiprep kit features a spin column-based method for the purification of up to 300 µg of high-quality plasmid DNA in less than 18 minutes. The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps.
ZymoPURE ™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl) directly from a spin column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA resulting in plasmid DNA suitable for sensitive applications including transfection, plasmid-mediated gene silencing, cloning, and sequencing.
As an added convenience, the ZymoPURE ™ Plasmid Midiprep contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Syringe filters are included for rapid clearing of the lysate and the unique spin-column design allows the binding step to be performed using a vacuum or table top centrifuge.
*Patent Pending