Member since: 2020
Organization: University of Idaho
Very useful kit for collecting all bacterial plasmids and chromosome.
Application Area: Plasmid biology
"I use this BAC miniprep kit all the time with gram negative bacteria containing plasmids of a variety of sizes (from 3kb up to 200kb). I can easily see all of the plasmids as well as the chromosome when running a few microliters of the eluted samples on an agarose gel. I get good results with PCR on these samples as well. There is definitely an issue with residual ethanol after sample elution but a 10-minute incubation at 55C solves this problem. You could also try an extra spin after the last wash (using an empty collection tube) if you don't want to heat your samples. "
The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.