YeastBuster™ Protein Extraction Reagent by Merck
Manufacturer Merck | Model: 71186
For fast, reproducible and gentle extraction of active proteins from yeast and plant cells, use YeastBuster™ Protein Extraction Reagent. This reagent avoids the harsh conditions of vigorous mechanical or chemical treatment that often result in degradation of target proteins. Harvest cells and resuspend in YeastBuster™ protein extraction reagent, incubate for 15 minutes and then remove cell debris by centrifugation. Your extract is now ready to use. The reagent has been tested with
YeastBuster™ Protein Extraction Reagent
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Average Rating: 5.0
1 Scientist has reviewed this product
Status:
Reviewer
Member since: 2018
Organization: Grifols
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- Ease of use
- After sales service
- Value for money
Good product.
Rating: 5.0
Application Area: Protein titer determination in yeast expression system
"Lysate procedure is easy, but need to confirm for inclusion body solubilization."
Review date: 11 Apr 2019 | YeastBuster™ Protein Extraction Reagent
Features & Benefits
- Eliminates the inconsistencies associated with abrasive grinding, ultrasonication, and pressure disruption of yeast cells
- Higher yield of total and enzymatically active proteins
- Efficient protein extraction from yeast under non-denaturing conditions from any volume of culture
- Add 0.5 M THP Solution (included) and Benzonase® Nuclease for enhanced efficiency
- Fully compatible with
- Ni-NTA His
- Bind® and GST
- Bind™ affinity purification method
