YeastBuster™ Protein Extraction Reagent by Merck

Manufacturer Merck  |   Model: 71186
5.0
/
5.0
  |  1 reviews
For fast, reproducible and gentle extraction of active proteins from yeast and plant cells, use YeastBuster™ Protein Extraction Reagent. This reagent avoids the harsh conditions of vigorous mechanical or chemical treatment that often result in degradation of target proteins. Harvest cells and resuspend in YeastBuster™ protein extraction reagent, incubate for 15 minutes and then remove cell debris by centrifugation. Your extract is now ready to use. The reagent has been tested with


YeastBuster™ Protein Extraction Reagent by Merck product image
YeastBuster™ Protein Extraction Reagent

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Average Rating: 5.0
1 Scientist has reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


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    Reviewer
  • Member since: 2018

  • Organization: Grifols



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good product.
Rating: 5.0

  • Application Area: Protein titer determination in yeast expression system

"Lysate procedure is easy, but need to confirm for inclusion body solubilization."

Review date: 11 Apr 2019 | YeastBuster™ Protein Extraction Reagent

Features & Benefits

  • Eliminates the inconsistencies associated with abrasive grinding, ultrasonication, and pressure disruption of yeast cells
  • Higher yield of total and enzymatically active proteins
  • Efficient protein extraction from yeast under non-denaturing conditions from any volume of culture
  • Add 0.5 M THP Solution (included) and Benzonase® Nuclease for enhanced efficiency
  • Fully compatible with
    • Ni-NTA His
    • Bind® and GST
    • Bind™ affinity purification method

Product Overview

YeastBuster™ Protein Extraction Reagent by Merck product image

YeastBuster™ Protein Extraction Reagent

Manufacturer Merck

5.0 / 5.0 | 1 reviews