YeastBuster™ Protein Extraction Reagent
For fast, reproducible and gentle extraction of active proteins from yeast and plant cells, use YeastBuster™ Protein Extraction Reagent. This reagent avoids the harsh conditions of vigorous mechanical or chemical treatment that often result in degradation of target proteins. Harvest cells and resuspend in YeastBuster™ protein extraction reagent, incubate for 15 minutes and then remove cell debris by centrifugation. Your extra…
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Good product.
Protein titer determination in yeast expression system
Lysate procedure is easy, but need to confirm for inclusion body solubilization.
Review Date: 11 Apr 2019 | Merck
Features & Benefits
- Eliminates the inconsistencies associated with abrasive grinding, ultrasonication, and pressure disruption of yeast cells
- Higher yield of total and enzymatically active proteins
- Efficient protein extraction from yeast under non-denaturing conditions from any volume of culture
- Add 0.5 M THP Solution (included) and Benzonase® Nuclease for enhanced efficiency
- Fully compatible with
- Ni-NTA His
- Bind® and GST
- Bind™ affinity purification method
