VAHTS Universal Plus DNA Library Prep Kit for Illumina by Vazyme Biotech Co., Ltd

2.3

5.0

VAHTS ® Universal Plus DNA Library Prep Kit for Illumina is a DNA library construction kit developed for the Illumina ® high-throughput sequencing platform.

VAHTS Universal Plus DNA Library Prep Kit for Illumina

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Description
Application Notes

Average Rating: 2.3

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2 out of 5
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Damiana Adamma Iwuala
Status:
Reviewer
Member since: 2021
Organization: Lab scientist

Ease of use

2 out of 5

After sales service

2 out of 5

Value for money

3 out of 5

Great results with good buffer

Rating: 2.3

Application Area: analyzer for RNA samples

"VAHTS universal V8 RNA-seq library prep kit for illumina is specially designed for the preparation of transcriptome libraries for illumina platform. The kit is universal and suitable for RNA library construction of RNA that have been obtained by poly(A)-based mRNA enrichment or RNA depletion, the kit contains two types of cDNA 2nd strand synthesis buffer, which can be chosen for library construction for non-stranded or stranded RNA-seq transcriptome analysis."

Review date: 26 May 2022 | VAHTS Universal Plus DNA Library Prep Kit for Illumina

VAHTS^® Universal Plus DNA Library Prep Kit for Illumina is a DNA library construction kit developed for the Illumina^® high-throughput sequencing platform. This kit combines DNA fragmentation, end repair, and dA tailing into one step. The product does not need to be purified. It is directly connected to adapters, library enrichment and sorting, and can convert 100 pg-1 μg template DNA into Illumina^® Qualcomm Volume sequencing platform dedicated library. There is no need to mechanically interrupt the genome, which simplifies the library construction process and shortens the operation time, and is suitable for PCR and PCR-Free library construction. This kit is perfectly compatible with different sources and different input amounts of DNA, and only needs to adjust the fragmentation time according to the target insert size to obtain the desired fragment size library.

Product Advantages

Wide compatibility: easy to deal with different species sources, different initial inputs and different template types
Simple operation: just adjust the fragmentation time according to the target insert fragment size to get the required library
High library yield: excellent library conversion rate and better sample quality

Applications

Applicable to DNA library preparation for NGS on Illumina^® platforms and compatible with 100 pg - 1 μg of input DNA and various kinds of DNA template including genomic DNA (from different species such as animals, plants, and microorganisms), paraffin sections DNA (FFPE DNA), etc.
It is recommended to use this kit for:

Whole genome sequencing
Whole exome or targeted sequencing (using IDT xGen™ Lockdown™ Probes, Roche^® NimbleGen™ SeqCap™ EZ or other hybridization capture systems).
Metagenome sequencing.
Methylation Sequencing (in combination with Phanta UC Super-Fidelity DNA Polymerase for Library Amplification, Vazyme #P507).

Notes:
The parameters of library preparation procedures may be adjusted according to sample types, experimental designs, instruments, and operations. For any questions during procedures, please contact Vazyme for help at global@vazyme.com.