TSKgel SW Series Gel Filtration Columns for Biomolecules
TSKgel SW-type columns for size exclusion/gel filtration analysis of antibodies and other proteins are silica-based. A hydrophilic diol-type bonded phase shields the silica surface from interacting with protein samples.
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Good quality, improvable batch to batch reproducibility.
High & low molecular weight heparin
The columns are easy to use but take care when doing so. Loss of resolution on the low molecular weight region (column bench 3000 + 2000) occurs very quickly, especially in mobile phase sodium phosphate. Ammonium acetate buffered at pH 6 so they are more stable. It seems like some particular stationary phase batches are more stable and reproducible than others. These columns have never been cheap.
Review Date: 7 Feb 2020 | Tosoh Bioscience - Separations
TSKgel SW-type columns for size exclusion/gel filtration analysis of antibodies and other proteins are silica-based. A hydrophilic diol-type bonded phase shields the silica surface from interacting with protein samples.
TSKgel SW-type columns contain more pore volume per unit column volume, which results in higher MW selectivity or better resolution. In addition, they show low adsorption and well-defined pore size distribution, which are requirements for high performance GFC of proteins.
Various pore sizes are available in TSKgel SW, SWXL, SuperSW, UltraSW and UP-SW product lines.
- TSKgel G3000SWXL is the working horse of the biopharmaceutical industry for aggregate analysis of therapeutic antibodies in QC.
- TSKgel UP-SW columns are optimized for use with UHPLC systems and allow a smooth transfer of standard methods from SWXL based HPLC analysis to UHPLC.
- TSKgel SuperSW mAb columns are optimized for antibody separation.
- TSKgel UltraSW Aggregate feature smaller particles and are optimized for analysis of higher aggregates.
Size Exclusion Chromatography
Size Exclusion (SEC) is the general name for the chromotographic mode also referred to as gel permeation chromatography (GPC) for non-aqueous elution systems or gel filtration chromatography (GFC) for aqueous systems.
SEC is a method in which components of a mixture are separated according to their molecular size. Large biomolecules that cannot penetrate the pores of the packing material elute first and flow with the mobile phase in the interparticle space of the packed column.
Smaller molecules can partially or completely enter the stationary phase and will elute from the column after the excluded sample components.
SEC is a very simple method for separating biomolecules, because it is not necessary to change the composition of the mobile phase during elution. However, the separation capacity of this method is limited.
