The TrueGel3D® HTS Hydrogel Plate is a ready-to-use solution for easily establishing 3D cell cultures using fully synthetic, PEG-based hydrogels in a simple and automation-compatible manner. These innovative hydrogels contain gradually increasing crosslinking densities throughout the well. Users can proceed directly with seeding cells without any hydrogel preparation or encapsulation steps. After cell seeding, cells will gradually infiltrate the hydrogel and establish a 3D cell culture environment within days. Additionally, this newly engineered hydrogel surface gives the user the possibility to sequentially establish co-culture systems by seeding different cell populations at different time-points in the same hydrogel well.
Compared to other hydrogels based on biological extracts from animal cells, TrueGel3D™ does not contain any products of animal origin that could interfere with or contaminate experiments. Hydrogel components preserve cell viability and allow critical features of the natural extracellular matrix (ECM) to be replicated to more accurately reflect native cellular environments similar to those of tissues by supporting cell adhesion and migration. TrueGel3D™ technology provides mechanical and biochemical cues to investigate both morphological and physiological properties of cells in the 3D environment.
Features and benefits: