Thermo Scientific™ SuperSignal™ ELISA Femto Substrate by Thermo Fisher Scientific

Manufacturer Thermo Fisher Scientific  |   Model: 37074
SuperSignal ELISA Femto Substrate is formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

Thermo Scientific™ SuperSignal™ ELISA Femto Substrate by Thermo Fisher Scientific product image
Thermo Scientific™ SuperSignal™ ELISA Femto Substrate

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SuperSignal ELISA Femto Substrate provides greater sensitivity in ELISAs or any other solution-based assay using chemiluminescent substrates. These ELISAs can take place in either a test tube or a microplate and is suitable for high-thoughput screening applications because of its improved enhancer system. SuperSignal ELISA Femto Substrate generates detectable light within one minute of addition to trace amounts of soluble HRP, saving up to 30 minutes per assay. The signal is quantified by measuring relative light units (RLU) in a luminometer.


  • Immediate light generation—intense signal generated immediately both at room temperature and 37°C; emits light at 425nm
  • Improved low-end linearity—easy detection of low quantities of proteins with high signal:noise ratios and low-end linearity of dose response curves
  • High sensitivity—femtogram-level detection of target proteins in ELISA
  • Reduction in assay time—high sensitivity allows for reduction in ELISA incubation steps
  • Stability—storage for six months at room temperature or a minimum of 12 months at 4°C with a six-hour working solution stability