Thermo Scientific™ LightShift™ Chemiluminescent EMSA Kit by Thermo Fisher Scientific

Manufacturer Thermo Fisher Scientific  |   Model: 20148
The LightShift Chemiluminescent EMSA Kit is an extraordinarily robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterize protein-DNA binding interactions.


Thermo Scientific™ LightShift™ Chemiluminescent EMSA Kit by Thermo Fisher Scientific product image
Thermo Scientific™ LightShift™ Chemiluminescent EMSA Kit

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The LightShift Chemiluminescent EMSA Kit includes reagents for setting up and customizing DNA binding reactions, a control set of DNA and protein extract to test the kit system, stabilized streptavidin-HRP conjugate to probe for the biotin-labeled DNA target, and an exceptionally sensitive chemiluminescent substrate module for detection. The assay requires purified DNA target that has been end-labeled with biotin, the protein extract to be tested, nylon membrane and basic electrophoresis equipment. DNA targets can be synthesized with 5' or 3' biotin labels or they can be labeled after synthesis using the Thermo Scientific Biotin 3' End DNA Labeling Kit (Product No. 89818). Nuclear, cytosolic or whole cell protein extracts can be obtained by a variety of methods, including the Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Reagent Kit (Product No. 78833).


Features:

  • Excellent for detecting low-abundance proteins in nuclear extracts
  • Sensitivity that surpasses radioactive and digoxigenin methods
  • Compatible with previously established binding conditions for popular DNA-protein interactions
  • Includes EBNA control system to help new users develop a working assay and understand the methods used to confirm binding interaction specificity