Symmetry Columns
Column-to-column and batch-to batch reproducibility
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Issue in the Column Efficiency
Facing issue in the peak shape
We are using Symmentry C8 150 * 4.6 5microns, faced the issue in tailing of the component. ( tailing was 3.8) Used the mobile phase ph 3.0 phosphate buffer and Acetonitrile in 70:30 ratio. Note: we used new column
Review Date: 14 Dec 2018 | Waters
Good result
Pharmaceutical analysis
It's very useful for our applications. In future we will try to use this columns
Review Date: 19 Dec 2017 | Waters
Review Date: 5 Aug 2011 | Waters
Symmetry™ LC Columns are manufactured using high purity silica and tightly controlled manufacturing processes to ensure that you receive a column that exceeds the standards for HPLC column performance.
No other silica-based LC column brand can match the column-to-column and batch-to-batch reproducibility of the Symmetry family. Symmetry Columns are available in column, cartridge, and guard formats to include:
- Symmetry C18 and C8 chromatography columns that deliver maximum reproducibility,
- SymmetryShield RP18 and RP8 chromatography columns to provide superior peak shape and
- Symmetry300 C18 and C4 chromatography columns, which offer high recoveries of peptides and proteins
A Practical Guide to the Effects of Water Purity On HPLC, LC-MS and Related Techniques
This guide from demonstrates the importance of using freshly produced ultrapure water to optimize performance when using liquid chromatography. The guide also contains general information about liquid chromatography, water purification technologies and how to avoid contamination.
Enhanced Detection of Host-Cell Proteins in Biotherapeutic Preparations using Preparative Electrophoresis Followed by LC–Ion Mobility-MS
This application note explains the fractionation of Host-cell proteins (HCPs) away from biotherapeutic protein using an off-line preparative electrophoresis device, allowing for sub-ppm level HCP detection. HCPs are proteins produced by the host cells during recombinant protein drug production and can contaminate the therapeutic drug protein even at very low levels. Detection of HCPs was improved through fractionating both the intact and reduced monoclonal antibody.
The pursuit of the perfect HPLC column: Reflecting on 50 years of innovation
Dr. Thomas H. Walter, of Waters Corporation, looks back at the trials, tribulations, and triumphs that paved the way to today’s advanced separation technologies
