StemSpan™ CD34+ Expansion Supplement (10X)
Serum-free culture supplement for expansion of human CD34+ hematopoietic cells
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StemSpan™ CD34+ Expansion Supplement typically promotes ~40-fold expansion of total nucleated cells in 7-day liquid cultures of CD34+ human cord blood (CB) cells. After one week, ~40% of the cultured cells express CD34, indicative of >10-fold expansion of input CD34+ CB cells. See data tab for more details. StemSpan™ CD34+ Expansion Supplement (10X) contains a combination of recombinant human cytokines (Flt3L, SCF, IL-3, IL-6 and TPO) and other additives formulated to selectively promote the expansion of CD34+ cells isolated from human CB and bone marrow (BM) samples. The CD34+ Expansion Supplement is intended for use in combination with StemSpan™ SFEM, SFEM II and -ACF serum-free expansion media, or any other media for culturing human hematopoietic cells.
When added to serum-free medium, the CD34+ Expansion Supplement typically promotes ~40-fold expansion of total nucleated cells in 7-day liquid cultures of CD34+ human CB cells. After one week, ~40% of the cultured cells express CD34, indicative of 10-fold expansion of input CD34+ CB cells. This supplement stimulates greater CD34+ cell expansion when compared to StemSpan™ CC100 and CC110. See data tab for more details.
