Stellaris RNA FISH by LGC, Biosearch Technologies

"Stellaris RNA FISH probes and reagent work well. I got great result."

"I have been a loyal Custom Stellaris FISH probe customer for the last 4 years and have ordered over 10 sets. Every interaction I've had with the customer service team has been extremely helpful and their replies have always been prompt (given the time zone difference). I highly recommend the Stellaris probe designer. It is both straight forward and intuitive to use. Negative tests that I've carried out have always confirmed probe set specificity (although I recommend carrying out a BLAST check for each set before placing an order.)"

"Low background and clean specific signal from these probes. Also great stability over time, providing long term consistent assay performance."

"The product seems to generally work as advertised, though it can be challenging to always understand how the Probe Designer generates custom probe sets, which can make troubleshooting and validation somewhat tricky at times."

"The Stellaris RNA FISH experiment gives out stable and repeatable results. I think the drawbacks of those RNA probes is that the green fluorescence is always hard to detected and showed great background, which means the limitation of color combination. But if you only need to check two different probes, the probes are really good and easy to use."

"Easy to order. The online application was user friendly. Only needed to input the sequence of the protein and the software took care of making the best probes for the sequence. Also, easy to use especially with the Stellaris FISH RNA kit."

"I did achieve high-quality results, with much less work than by traditional methods (ie northern, qPCR) plus with the bonus of spatial information. Other users should know that the difficulty of this method is directly proportional to the abundance of your target RNA. I did find the fluorophore nomenclature a bit different that I was used to, but all the resources to figure it out are on the website..."

"The Stellaris FISH probes are easy to use and the data are quantitative and reproducible."

"Amazing product, very reliable and easy to use. Gives consistent results and easily quantifyable using software such as FIJI. However it's expensive, and half-life of signal is too short so you have to image the samples the same or next day, and re image after one week can sometimes lead to no signal."