Stellaris RNA FISH by LGC

Manufacturer LGC  |  Available Worldwide
4.3
/
5.0
  |  16 reviews
RNA visualization method that allows simultaneous detection, localization, and quantification of individual mRNA molecules


Stellaris RNA FISH by LGC product image
Stellaris RNA FISH

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Average Rating: 4.3
16 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2017

  • Organization: University of California at Santa Barbara



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Excellent performance
Rating: 4.3

  • Application Area: Single molecule RNA FISH

"I purchased premade hSOX2 FISH probes and it worked really well with very low background. I likes the quality of these probes since smFISH is a very sensitive experiment."

Review date: 01 Nov 2017 | Stellaris RNA FISH
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: ST.Jude



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great Results.
Rating: 5.0

  • Application Area:Detect RNA with single molecular FISH

"Stellaris RNA FISH probes and reagent work well. I got great result."

Review date: 01 Nov 2017 | Stellaris RNA FISH
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Earlham Institute, Norwich Research Park, UK



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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I love custom Stellaris FISH probes!
Rating: 5.0

  • Application Area:Quantification of RNA in plant cells

"I have been a loyal Custom Stellaris FISH probe customer for the last 4 years and have ordered over 10 sets. Every interaction I've had with the customer service team has been extremely helpful and their replies have always been prompt (given the time zone difference). I highly recommend the Stellaris probe designer. It is both straight forward and intuitive to use. Negative tests that I've carried out have always confirmed probe set specificity (although I recommend carrying out a BLAST check for each set before placing an order.)"

Review date: 01 Aug 2017 | Stellaris RNA FISH
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Altius Institute for Biomedical Sciences



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Solid clean probes
Rating: 4.7

  • Application Area:FISH assays on tissue culture cells

"Low background and clean specific signal from these probes. Also great stability over time, providing long term consistent assay performance."

Review date: 31 Jul 2017 | Stellaris RNA FISH
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: UC Berkeley



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Consistent and straightforward
Rating: 4.0

  • Application Area:Cultured human cells

"The product seems to generally work as advertised, though it can be challenging to always understand how the Probe Designer generates custom probe sets, which can make troubleshooting and validation somewhat tricky at times."

Review date: 31 Jul 2017 | Stellaris RNA FISH
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Division of Biology Sciences, University of California, San Diego



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Great Probes!
Rating: 4.7

  • Application Area:Analyze gene expresssion pattern in single cell

"The Stellaris RNA FISH experiment gives out stable and repeatable results. I think the drawbacks of those RNA probes is that the green fluorescence is always hard to detected and showed great background, which means the limitation of color combination. But if you only need to check two different probes, the probes are really good and easy to use."

Review date: 31 Jul 2017 | Stellaris RNA FISH
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: UCLA



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
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Great results
Rating: 3.3

  • Application Area:In situ hybridization

"Easy to order. The online application was user friendly. Only needed to input the sequence of the protein and the software took care of making the best probes for the sequence. Also, easy to use especially with the Stellaris FISH RNA kit."

Review date: 31 Jul 2017 | Stellaris RNA FISH
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: The Rockefeller University



  • Ease of use
    4 out of 5
    After sales service
    1 out of 5
    Value for money
    5 out of 5
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Great complimentary approach to old school gel-based methods...
Rating: 3.3

  • Application Area:RNA FISH to quantify/visualize lncRNA of interest

"I did achieve high-quality results, with much less work than by traditional methods (ie northern, qPCR) plus with the bonus of spatial information. Other users should know that the difficulty of this method is directly proportional to the abundance of your target RNA. I did find the fluorophore nomenclature a bit different that I was used to, but all the resources to figure it out are on the website..."

Review date: 31 Jul 2017 | Stellaris RNA FISH
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: University of South Carolina



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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So far I am happy with the results and I would be continuing to use this product.
Rating: 4.0

  • Application Area:In vitro cell cultures and cryosectioned tissue samples.

"The Stellaris FISH probes are easy to use and the data are quantitative and reproducible."

Review date: 31 Jul 2017 | Stellaris RNA FISH
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: The Ritchie Center, Hudson Institute of Medical Research



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    2 out of 5
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Great product, but expensive and could be improved.
Rating: 3.7

  • Application Area:Analyse gene expression in tissue

"Amazing product, very reliable and easy to use. Gives consistent results and easily quantifyable using software such as FIJI. However it's expensive, and half-life of signal is too short so you have to image the samples the same or next day, and re image after one week can sometimes lead to no signal."

Review date: 31 Jul 2017 | Stellaris RNA FISH


  • Status:

    Reviewer

  • Member since: 2017

  • Organization: University of Basel , Biozentrum



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
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Decent results , will hope for improvement in later experimentd
Rating: 3.7

  • Application Area:Single molecule RNA FISH for localization analysis

"The Stellaris probes were used with commecially available products . Initial experiments were decent however a major obstacle was the lack of imaging with confocal microscopes. This leads to the acquisition of signal with widefield microscope and deconvolution which is still not very satisfactory"

Review date: 31 Jul 2017 | Stellaris RNA FISH
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Whitehead Institute for Biomedical Research



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product. Highly recommended.
Rating: 5.0

  • Application Area:Visualize RNA expression

"The smFISH probes are a bit expensive but they worked perfectly. The design interface was also very easy to use."

Review date: 31 Jul 2017 | Stellaris RNA FISH
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: Umass Medical School



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great tool for RNA studies
Rating: 4.7

  • Application Area:visualize RNA in fixed cells

"Stellaris FISH probes are a great tool to visualize RNA in fixed cells. The protocol in straightforward to use and yields very clear and specific signal."

Review date: 31 Jul 2017 | Stellaris RNA FISH
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: University of Illinois at Urbana-Champaign



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Expertise in FISH probes!
Rating: 5.0

  • Application Area:Analyze single molecule RNA in cells

"I've ordered quite a few sets of RNA FISH probes for single molecule RNA imaging in cells. I've also used the design tool for probe design. They work every well. I've gotten quite a lot amazing results using the probes."

Review date: 31 Jul 2017 | Stellaris RNA FISH
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: EMBL



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
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Adequate results with very little work
Rating: 4.0

  • Application Area:Drosophila RNA smFISH

"The FISH probes are very easy to use, the costum probe looked very good straight away, the GAPDH positive control did not work so well however. A detailed protocol for Drosophila oocytes for beginners would be helpful and very easy to include from the literature."

Review date: 31 Jul 2017 | Stellaris RNA FISH
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: KU Leuven



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great
Rating: 5.0

  • Application Area:LncRNA detection

"It is super easy to use and by labelling the same probeset with biotin I can do also pulldowns"

Review date: 31 Jul 2017 | Stellaris RNA FISH
 
Stellaris FISH (fluorescence in situ hybridization) is a RNA visualization method that allows simultaneous detection, localization, and quantification of individual RNA molecules at the sub-cellular level in fixed samples using widefield fluorescence microscopy.
 
A set of Stellaris FISH Probes comprises multiple oligonucleotides with different sequences each with a fluorescent label that collectively bind along the same target transcript to produce a punctate signal.
 
The Stellaris FISH technology follows a simple protocol, does not use exotic reagents, is inexpensive and platform-independent, offers same-day results, and is versatile toward many sample types and applications. Scientists may even label Stellaris FISH probes with a number of various dyes to allow for multiplex detection of different RNA targets simultaneously. Finally, scientists can address the stochastic nature of gene expression and visualize RNA through direct detection without isolation, purification, and amplification.