Member since: 2017
A new way to look at kinetics of binding to cell surface targets
Application Area: kinetics analysis of large and small molecules to cell membrane targets
"We have been using the SPRm 200 instrument in our lab for several years now. The combination of microscopy and SPR are an enticing feature of the technology allowing us to perform kinetic binding studies of large and small molecules to targets on cells. This had been very helpful in our efforts. unlike a traditional biosensor which use primarily recombinant protein, here you are working with cells. Having access to those with cell culture knowledge is helpful in this respect. Once completed though you can then inject your analytes over the cells and investigate the binding response. This instrument has a lot to offer in this area of drug development."
The Surface Plasmon Resonance Microscopy (SPRM) system, integrates optical microscopy and SPR. It is a powerful technique for measuring the binding activities of membrane proteins in vitro. It allows the simultaneous measurement of phenotypical changes of the sample via bright field and binding strength and kinetics via SPR.