SingleShot™ SYBR® Green Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions
SingleShot™ SYBR® Green Cell Lysis and RT-qPCR Kit, 100 x 50 µl reactions
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Impressed with the reproducible and sensitive results obtained
transcriptomic analysis of macrophages
I recently tried the SingleShot™ SYBR® Green Kit for Cell Lysis and RT-qPCR, and I have to say I'm thoroughly impressed! The kit is incredibly easy to use, and the results are fantastic. The cell lysis buffer worked like a charm, and the RT-qPCR reactions were highly specific and sensitive. I was able to get reproducible results with minimal effort, and the 100 x 50 µl reactions size is perfect for my needs. Overall, I highly recommend this kit to anyone looking for a reliable and efficient way to perform cell lysis and RT-qPCR. It's truly a game-changer!
Review Date: 17 Apr 2023 | Bio-Rad
Use the SingleShot™ SYBR® Green Kit to obtain high-quality RT-qPCR data with SYBR® Green chemistry directly from cell culture lysates.
Two-step workflow provides results less than 2 hours after cell lysis. All RT-qPCR reagents and RNA control included.
Benefits include:
- Cell lysate from 10 to 100,000 cells ready in 20 minutes
- Complete removal of genomic DNA while preserving RNA integrity
- Optimal accuracy and linear dynamic range of qPCR data across genes
- Results comparable to using purified RNA, including dynamic range
- Validated with PrimePCR™ Assays and Panels (visit www.bio-rad.com/primepcr )
- SingleShot RNA Control ensures optimal cell and lysate inputs
Optimized Sample Input
The SingleShot™ SYBR® Green Kit maximizes cell lysate and cDNA inputs in a two-step RT-qPCR reaction:
Kit Contents
Refer to the user manual for details on reaction setup and troubleshooting tips.
Cell Lines Validated for Use with the SingleShot™ SYBR® Green Kit
The SingleShot™ SYBR® Green Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:
Related Products
The SingleShot™ SYBR® Green Kit belongs to the family of SingleShot™ Cell Lysis RT-qPCR Kits :
- SingleShot Cell Lysis One-Step RT-qPCR Kits — including SingleShot™ SYBR® Green One-Step Kit ( 172-5095 ) and SingleShot Probes One-Step Kit ( 172-5070 )
- SingleShot Cell Lysis RT-qPCR Kits — including SingleShot™ SYBR® Green Kit ( 172-5085 ) and SingleShot Probes Kit ( 172-5090 )
- SingleShot Cell Lysis Kit — available for 100 reactions ( 172-5080 )
