Member since: 2021
Organization: IIT Kanpur
Impressed with the reproducible and sensitive results obtained
Application Area: transcriptomic analysis of macrophages
"I recently tried the SingleShot™ SYBR® Green Kit for Cell Lysis and RT-qPCR, and I have to say I'm thoroughly impressed! The kit is incredibly easy to use, and the results are fantastic. The cell lysis buffer worked like a charm, and the RT-qPCR reactions were highly specific and sensitive. I was able to get reproducible results with minimal effort, and the 100 x 50 µl reactions size is perfect for my needs. Overall, I highly recommend this kit to anyone looking for a reliable and efficient way to perform cell lysis and RT-qPCR. It's truly a game-changer!"
Use the SingleShot™ SYBR® Green Kit to obtain high-quality RT-qPCR data with SYBR® Green chemistry directly from cell culture lysates.
Two-step workflow provides results less than 2 hours after cell lysis. All RT-qPCR reagents and RNA control included.
The SingleShot™ SYBR® Green Kit maximizes cell lysate and cDNA inputs in a two-step RT-qPCR reaction:
Refer to the user manual for details on reaction setup and troubleshooting tips.
The SingleShot™ SYBR® Green Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:
The SingleShot™ SYBR® Green Kit belongs to the family of SingleShot™ Cell Lysis RT-qPCR Kits :