SingleShot™ Probes One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions by Bio-Rad

Manufacturer Bio-Rad  |   Model: 1725070
5.0
/
5.0
  |  1 reviews
SingleShot™ Probes One-Step Cell Lysis and RT-qPCR Analysis Kit, 100 x 50 µl reactions


SingleShot™ Probes One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions by Bio-Rad product image
SingleShot™ Probes One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions
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Average Rating: 5.0
1 Scientist has reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


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  • Organization: University of Cape Town



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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excellent results
Rating: 5.0

  • Application Area: Gene expression and amplifications

"Very reliable, accurate and worth its costs and values"

Review date: 03 Mar 2017 | SingleShot™ Probes One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

Use the SingleShot Probes One-Step Kit to obtain high-quality RT-qPCR data with probe chemistry directly from cell culture lysates in a one-step workflow 1 hour after cell lysis. All reagents and RNA control included.

Benefits include:

  • Cell lysate from 10 to 100,000 cells ready in 20 minutes
  • Complete removal of genomic DNA while preserving RNA integrity
  • Optimal accuracy and linear dynamic range of qPCR data across genes
  • Results comparable to using purified RNA, including dynamic range
  • Validated with PrimePCR™ Assays and Panels (visit www.bio-rad.com/primepcr )
  • SingleShot RNA Control ensures optimal cell and lysate inputs

Optimized Sample Input

The SingleShot Probes One-Step Kit maximizes cell lysate input in a one-step RT-qPCR reaction:


Kit Contents




Refer to the user manual for details on reaction setup and troubleshooting tips.

Cell Lines Validated for Use with the SingleShot Probes One-Step Kit

The SingleShot Probes One-Step Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:

 

Related Products

The SingleShot Probes One-Step Kit belongs to the family of SingleShot™ Cell Lysis RT-qPCR Kits :

  • SingleShot Cell Lysis One-Step RT-qPCR Kits — including SingleShot™ SYBR® Green One-Step Kit ( 172-5095 ) and SingleShot Probes One-Step Kit ( 172-5070 )
  • SingleShot Cell Lysis RT-qPCR Kits — including SingleShot™ SYBR® Green Kit ( 172-5085 ) and SingleShot Probes Kit ( 172-5090 )
  • SingleShot Cell Lysis Kit — available for 100 reactions ( 172-5080 )

Product Overview

SingleShot™ Probes One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions by Bio-Rad product image

SingleShot™ Probes One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

Manufacturer Bio-Rad

5.0 / 5.0 | 1 reviews