Seahorse XF Palmitate Oxidation Stress Test Kit and FAO Substrate
The Agilent Seahorse XF Palmitate Oxidation Stress Test Kit provides validated reagents to measure long chain fatty acid oxidation (FAO) via assessing changes in oxygen consumption (OCR) in intact cells. The kit includes the XF Palmitate-BSA FAO Substrate, L-carnitine, etomoxir, as well as oligomycin, FCCP, and rotenone/antimycin A to determine the intrinsic rate and capacity of a cell to oxidize palmitate in the absence or l…
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The Seahorse XF Palmitate-BSA FAO Substrate included in the kit is pre-conjugated with a quantified free palmitate concentration eliminating the tedious, time-consuming, and variable conjugation process. The Palmitate-BSA reagent is also sold independently for use in other types of assays.
Features
- Discover how cell phenotype, function or fate can be controlled via manipulation or control of fatty acid oxidation activities
- Build on the well-established XF assay (XF Mito Stress Test) for interrogating mitochondrial metabolism for gaining deep insights into specific effects of genetic manipulations and/or drug exposure on the long chain fatty acid oxidation process
- A complete solution for specifically measuring oxidation of long chain fatty acids (palmitate), under basal respiration and high energy demand conditions. Includes Palmitate-BSA substrate, carnitine, etomoxir, oligomycin, FCCP, and rotenone/antimycin-A
- Use as a complementary and/or follow assay to the XF Long Chain Fatty Acid Oxidation Stress Test (103672-100)
- Obtain reliable, real-time, functional information about FAO using intact cells without radioactivity or molecular labels
- Ready-to-use Palmitate-BSA substrate for consistent results in every assay, and with free fatty acid concentration provided for every lot
- Compounds are reconstituted directly in the assay medium, eliminating the need for DMSO or other solvents
- Every lot is rigorously tested and validated with functional assays to deliver consistent and robust results
- Upload your assay result file to your Agilent Seahorse Analytics account to calculate and report parameters of the XF Substrate Oxidation Stress Test, share with collaborators, or export data to Microsoft Excel or GraphPad Prism for other graphing or analysis needs
Agilent Seahorse XF Palmitate Oxidation Stress Test kit
In this application note, Agilent Technologies introduces the Agilent Seahorse XF Palmitate Oxidation Stress Test kit, which provides validated reagents for measuring palmitate oxidation in live cells. The assay, built on the Seahorse XF Cell Mito Stress Test, assesses changes in oxygen consumption rate (OCR) and enables easy determination of a cell's intrinsic rate and capacity to oxidize palmitate. Researchers can gain insights into how interventions impact long-chain fatty acid (LCFA) oxidation, offering potential therapeutic implications. The kit includes ready-to-use reagents and a cloud-based analytical tool for quick and organized results.
Revealing cellular metabolic phenotype and function using Agilent XF substrate oxidation stress tests
In this application note, Agilent Technologies showcases its Seahorse XF technology, which enables real-time measurement of energy metabolism in live cells, offering vital functional insights related to cellular health and fitness. The Agilent Seahorse XF Substrate Oxidation stress test provides essential metrics for investigating specific mitochondrial substrates that impact cellular phenotype and function. The study includes an assay strategy overview, data examples, and guidance on applying these methods in cancer and immunological cell metabolism research for drug discovery.
