Agilent Seahorse XF Palmitate Oxidation Stress Test kit
31 Jul 2023In this application note, Agilent Technologies introduces the Agilent Seahorse XF Palmitate Oxidation Stress Test kit, which provides validated reagents for measuring palmitate oxidation in live cells. The assay, built on the Seahorse XF Cell Mito Stress Test, assesses changes in oxygen consumption rate (OCR) and enables easy determination of a cell's intrinsic rate and capacity to oxidize palmitate. Researchers can gain insights into how interventions impact long-chain fatty acid (LCFA) oxidation, offering potential therapeutic implications. The kit includes ready-to-use reagents and a cloud-based analytical tool for quick and organized results.
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Seahorse XF Palmitate Oxidation Stress Test Kit and FAO Substrate
Agilent TechnologiesThe Agilent Seahorse XF Palmitate Oxidation Stress Test Kit provides validated reagents to measure long chain fatty acid oxidation (FAO) via assessing changes in oxygen consumption (OCR) in intact cells. The kit includes the XF Palmitate-BSA FAO Substrate, L-carnitine, etomoxir, as well as oligomycin, FCCP, and rotenone/antimycin A to determine the intrinsic rate and capacity of a cell to oxidize palmitate in the absence or limitation of other exogenous substrates. This kit is best applied when