The Sage Pippin instruments are used to prepare accurate, reproducible and reliable genomic DNA (or cDNA) fragment libraries for subsequent NGS. Further to accurate DNA (or cDNA) size selection, the Pippin instruments remove potential contaminants, including primer dimers (introduced during the PCR reaction) or adaptor dimers (introduced during the adaptor ligation step):
Sage produces a range of Pippin models depending upon your application needs:
Pippin Prep – Automated size selection of DNA fragments between 100bp and 1.5kb for short-read NGS.
Blue Pippin – Incorporating pulse-field technology for accurate and reproducible size selection between 100bp up to 50kb and High-Pass filtering (addresses both short and long-read NGS).
Pippin HT - Automated size selection of up to 24 samples in parallel. Incorporates pulse-field technology to allow size selection between 100bp to 1.5 kb and High-Pass filtering
Pippin Pulse – An electrophoresis power supply incorporating the Sage pulse-field technology. It allows users to resolve DNA up to 450kb in their analytical gels.