RNA Clean & Concentrator™-5 by Zymo Research

Manufacturer Zymo Research  |   Model: R1013
4.7
/
5.0
  |  31 reviews
The RNA Clean & Concentrator™-5 cleans and concentrates up-concentration at 10 µg of RNA in as little as 5 minutes.


RNA Clean & Concentrator™-5 by Zymo Research product image
RNA Clean & Concentrator-5
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Average Rating: 4.7
31 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2021

  • Organization: UPF



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Quicker than traditional ethanol precipitation and recovery of 100% for RNA.
Rating: 5.0

  • Application Area: Molecular biology

"This kit is great to concentrate samples after poly(A) selection. I use it all the time so that I can have 5ugs of poly(A)selected RNA in 15ul volume without the need of the tradicional ethanol precipitation with glycogen. This way your sample is pure and without glycogen. the value for money is also very good."

Review date: 16 Jun 2021 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: University of Wisconsin



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Quintessential for RNAseq prep!
Rating: 5.0

  • Application Area:RNA sample preparation for sequencing

"This kit is allows for me to clean and concentrate RNA from nascent zebrafish embryos which have an abundance of yolk that can contaminate sample preparations."

Review date: 09 Jun 2021 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: University of Maine



  • Ease of use
    5 out of 5
    After sales service
    2 out of 5
    Value for money
    5 out of 5
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Great results.
Rating: 4.0

  • Application Area:RNA extraction for qPCR

"This kit is easy to use. I used it to extract RNA for qPCR and this kit worked."

Review date: 07 Jun 2021 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Auburn University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Excellent product.
Rating: 5.0

  • Application Area:RNA isolation

"The best kit for RNA isolation, which will provide super-pure RNA products in a couple of minutes, is widely used in our daily work. It will provide more than 90% recovery of RNA product from enzymatic digestion, ligation, etc. "

Review date: 26 May 2021 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Beckman Coulter



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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Will continue to use this kit for similar assays in the future.
Rating: 3.7

  • Application Area:Analysis of RNA in plants

"The product has proved very effective, easy to use and efficient for my experiments. However, when I first received the kit, one bottle was unlabeled, so I had to contact customer service in order to get it replaced."

Review date: 26 May 2021 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Auburn University



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Great kit! We use it everyday!
Rating: 4.3

  • Application Area:RNA purification

"My lab's product of choice for total RNA purification after phenol-chloroform extraction! We rely on this kit to effectively regain the most amount of input RNA compared to other purification kits. Much quicker than EtOH precipitation, as well! The kit lasts a long time in our lab, even though we constantly use it between 4 graduate students! The only issue we have is that if you're not careful, the filters will come out and you could lose your product."

Review date: 26 May 2021 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Donald Danforth Plant Science Center



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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I will continue to use this kit for similar assays.
Rating: 3.7

  • Application Area:Analyze RNA from plant chloroplasts

"The kit is easy to use and seems to be effective at cleaning my RNA samples. When I first received the kit, one of the bottles was unlabeled, so I had to contact customer service to get it replaced. I was still able to use the kit and will continue to order it as long as I am working on RNA experiments."

Review date: 20 May 2021 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Auburn University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Dependable and effective!
Rating: 4.7

  • Application Area:Total RNA extraction from BCBL-1 cells.

"We use this product for total RNA extraction of BCBL-1 cells! We preferentially use this kit above all other purification methods because of great results, the quick protocol, and ease of use!"

Review date: 20 May 2021 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Uniformed Services University Health Sciences Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results! It was a saver.
Rating: 5.0

  • Application Area:Biomarkers in tissue samples

"The product was great. It was very easy to use and I was able to purify the RNA sample. The quality was very good after using this kit. I shall use this in future if needed. I highly recommend it for purifying RNA samples."

Review date: 16 Mar 2021 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Yale University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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It’s a must have in an RNA lab.
Rating: 4.7

  • Application Area:Analyze RNA via qPCR, RNA-sew, and ribosome profiling

"Very easy to use for many downstream applications. It makes what could take forever take just minutes and results in clean RNA. Results are consistent and the recovery is fantastic. I just wish we could get more columns as we have a lot of buffers left over."

Review date: 16 Mar 2021 | RNA Clean & Concentrator™-5


  • Status:

    Reviewer

  • Member since: 2020

  • Organization: UCLA



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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Work well, easy and fast!
Rating: 4.0

  • Application Area:Analyze gene expression in the liver

"Worked very well, total amount of RNA may decrease a little bit."

Review date: 04 Dec 2020 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Stanford University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Very useful tool for transcriptome study using low input samples.
Rating: 5.0

  • Application Area:Study the transcriptome of microglia cells in spinal cord in animal pain model

"The RCC-5 kit can effectively purify high quality total RNA from a very low amount of cells (~ 4000) for RNA-seq. It's convenient and cost-effective for transcriptome study. The sales team were supportive by providing samples and suggestions for optimization."

Review date: 04 Dec 2020 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Smith College



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great kit and great value with it's ease of use and robust, reproducible results.
Rating: 5.0

  • Application Area:RNA preparation from E. coli for RNA-Seq analysis

"We used the RNA Clean and Concentrator kit to clean up RNA after a DNase treatment during an RNA-Seq class with 6 students. From there, we were able to either perform another DNase treatment, if needed, or go directly to library prep for next-generation sequencing. We found that the Zymo kit was quicker and easier to use, and therefore gave us more robust and reproducible results, with minimal loss of sample, than other kits we had used in the past, making it a better value for us as students were less likely to introduce error into the processing of their samples. Zymo was also very responsive in asking questions about how to accommodate very large or small sample volumes or amounts."

Review date: 08 Jun 2020 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: UNSW Sydney



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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RNA Clean & Concentrator™-5 product is quick, easy to use, and effective!
Rating: 4.7

  • Application Area:Cleaning up of 30 nt RNA fragments

"The RNA Clean & Concentrator™-5 product has helped streamline my experiments by reducing time during intermediate steps where I had to clean up my RNA species after several reactions."

Review date: 15 Jan 2020 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Wisconsin - Madison



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results - streamlined my workflow!
Rating: 5.0

  • Application Area:Library prep for sequencing

"Great results! Super at concentrating your RNA for downstream sequencing. Really easy to use!"

Review date: 11 Dec 2019 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Iowa State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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The best kit for RNA purification.
Rating: 5.0

  • Application Area:Purification of in vitro transcribed RNAs, clean up RNA after DNAse treatment etc.

"Compared to other brands' kits in the market, kits from Zymo, including RNA Clean and Concentrator are very to use, quicker, and give phenomenal quality and consistent results. Zymo kits are very cost-effective as well."

Review date: 27 Nov 2019 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Stem Pharm



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Excellent.
Rating: 5.0

  • Application Area:RNA extraction

"Excellent product."

Review date: 10 Nov 2019 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Iowa State University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Great kits. Have recommended to several colleagues and friends.
Rating: 4.7

  • Application Area:RNA sequencing, in vitro purification, etc.

"High quality, reproducible results. Cost-effective. Low elution volume is the main strength of the columns."

Review date: 05 Jun 2019 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Chapman University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great for RNA extraction, not only cleaning and concentrating!
Rating: 5.0

  • Application Area:RNA extraction from Trizol preserved tissues

"This kit has worked awesomely for me. I use it for RNA extraction from Trizol preserved tissues. I do the chloroform step first and then proceed with this kit, using the top transparent layer (RNA). Results are consistently good, but a great advantage of the kit is that if a result happens to be less good, you just run the protocol again through a new column to clean and concentrate the RNA (the original purpose of the kit!)."

Review date: 05 Jun 2019 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Iowa State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Good service.
Rating: 4.7

  • Application Area:Separate RNA from a mixture (RNA SHAPE)

"I get high quality RNA using this kit. Initially my experiment was not working due to lack of pure and high quality RNA but after using this kit, my experiment worked."

Review date: 05 Jun 2019 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: IZSPLVA



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great kit to increase RNA concentration for precious samples!
Rating: 5.0

  • Application Area:Concentration of RNA and DNAse digestion in difficult samples

"I used this kit to improve the quality and concentration of RNA obtained with trizol or equivalent from difficult samples before starting NGS library prep and I got very good results in terms of yield and purity. Sometimes I also applied the on-column DNAse digestion and it was ok."

Review date: 05 Jun 2019 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Georgia



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great item in the lab.
Rating: 5.0

  • Application Area:Isolate PCR-inhibitor free RNA on plants

"Very easy to use. Instructions are simple to follow. Wish the protocol could be written in more of stream-lined way. Crisitian was an Amazing sales rep and made sure I had the right product for my project."

Review date: 05 Jun 2019 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: USAMRIID



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great for purifying small amounts of RNA
Rating: 5.0

  • Application Area:Purify small amounts of RNA

"These kits are great for purifying RNA. I use a bioanalyzer after and I can see the difference from before, cleaner bands and the concentration increases with a smaller amount of elutant."

Review date: 15 May 2017 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: OMRF



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Zymo RNA clean & Concentrator is the best product CRISPR applications
Rating: 5.0

  • Application Area:Guide RNA purification

"We use RNA clean & Concentrator 5 for cleaning up guide RNA after in vitro transcription reaction. We have tried several other products including precipitation methods, Zymo product gave us the best results and yield."

Review date: 23 Jan 2017 | RNA Clean & Concentrator™-5
  • Status:

    Advanced Reviewer

  • Member since: 2008

  • Organization: Swansea University



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
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Effective and easy to use
Rating: 3.7

  • Application Area:Preparation of RNA for expression profiling

"Easy to use, cleaned up our recalcitrant samples well, a useful standby"

Review date: 18 Jan 2017 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Southern California



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Surprisingly good yields and results.
Rating: 5.0

  • Application Area:RNA clean up

"This kit is easy to use and is a very fast process to provide high efficient yields. Even with a low amount of input, the yields are still amazing good. Everybody would love it after trying it once."

Review date: 30 Sep 2016 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Zaragoza, Spain



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Good kit, does what it promises with good recovery. Can be also used for in column DNase treatment.
Rating: 4.3

  • Application Area:Concentration of RNA samples and treating them with DNAse I

"Easy to use kit. My primary purpose was to DNAse treat the RNA samples that were extracted before, and this kit seems suitable for that - taken that you have DNAse and its buffer from some other Zymo kit. The sample recovery in my hands was in the range of 64% - 99.5% of the input, with an average of 78% (n= 12). I know that the samples contained very little DNA so this output is probably quite close to the recovery % even without DNAse treatment. So taken that minimum recovery what you expect is around 60%, it is best to double the input with respect to the amount of RNA required after the clean up. The OD 260/280 was not affected. However, OD 260/230 decreased in all samples from an average of 2.2 (range 2.11 - 2.37) before to an average of 1.75 (range 1.44 - 1.96) post treatment. This decrease in OD 260/230 was observed also when centrifugation time was extended in order to better remove remaining remnants of buffer that might interfere with A230. I'm not personally worried about this but it may be good to have a look in case extra pure RNA is needed."

Review date: 19 Sep 2016 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Oxford University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Highly preferable to Trizol
Rating: 4.7

  • Application Area:RNA purification

"Really easy and quick to use, great quality RNA, very reproducible; low elution volume for low yield samples really useful. No phenol necessary and compatible if sample is already in a different buffer."

Review date: 28 Jul 2016 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Inserm U955, EQ15



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Very efficient in concentrating RNA with low loss of material.
Rating: 4.3

  • Application Area:Concentrate RNA for RNA-Seq experiments

"It is very easy to use and there is only a slight amount of RNA that is lost. It is very convenient for small amount of RNA, but not really adapted to larger volume. A bigger size of column might be proposed. DNase provided in the kit to remove DNA is not enough for the amount of RNA we use. "

Review date: 28 Jul 2016 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Case Western Reserve University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Easy to use.
Rating: 4.7

  • Application Area:RNA Extraction

"Product is easy to use, and easy to understand. It is useful for getting more concentrated RNA from samples."

Review date: 21 Jul 2016 | RNA Clean & Concentrator™-5
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Wisconsin-Madison



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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This could be a more useful kit if I didn't lose so much RNA to the column.
Rating: 4.3

  • Application Area:Preparation of RNA samples for downstream gene expression analysis.

"This kit was one of several I tested back-to-back when comparing options for RNA purification upstream of qPCR. Ultimately, I was looking for something easy and quick to use (for higher throughput with many samples) that would leave me with good quality and reasonably abundant RNA. I used this as a clean-up step after guanidinium thiocyanate-phenol-chloroform extraction from homogenized tissues. I found the process very simple, and adding the kit on did seem to significantly improve the quality of the RNA pool relative to the original phenol-chloroform extraction. However, I lost quite a bit of RNA to the column. I did not end up including the kit in my final workflow because of this issue, even though I was happy with the RNA quality it yielded. "

Review date: 18 Jul 2016 | RNA Clean & Concentrator™-5
RNA Clean & Concentrator ™ -5 provides a simple and reliable method for the rapid preparation of up to 10 µg of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA.  This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy:  Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA.  The RNA can be eluted from the Zymo-Spin ™ IC Column in as little as ≥6 µl of RNase-free water.  The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations. The entire procedure typically takes about 5 minutes.

Product Overview

RNA Clean & Concentrator™-5 by Zymo Research product image

RNA Clean & Concentrator™-5

Manufacturer Zymo Research

4.7 / 5.0 | 31 reviews