Quick-RNA™ Miniprep Plus by Zymo Research

"Easy to use."

"In our lab, we always use Zymo RNA isolation kit while dealing with important samples. Its a real blessing to have this awesome, time saving, highly reproducible kit which always gives high quality RNA."

"It is a great kit (R1057) compared with the Miniprep one (R1054). I used both, and this kit helped to remove the protein contamination and the purity was good. To identify the quality, I used Nanodrop to test the A260/A230 and A260/A280, and gel electrophoresis applied, the ratio of 28S/18S was 2:1. Also, some samples were analyzed by 2100 bioanalyzer, the RINs were in 7-8.5. Read the protocol carefully and it easy to follow. One suggestion is to use the RNase AWAY to clean the environment and pipettes before the isolation. Another is that do not use too many samples, I tried to use different amount samples including liver, brain, and testes, less than 50 mg was good enough, more samples may result in less production. It is a good idea if you used DNA/RNA shield (or RNA latter) to store samples, if not please do not store those samples too long. I choose the Miniprep kit first because of the low price, but the results were not ideal, and I send the result to the Zymo. They helped to analyze the result and made good suggestions and send me samples of the Plus kit for free. It really helped my study. I recommend the plus kit (R1057)."

"I have used many different kits from different suppliers, I have struggled a lot with RNA quality from small amounts of cultured cells when using other kits; however, with this Quick RNA miniprep plus kit, the quality has been excellent! My ratios never fell below 2. The quantity of RNA is more consistent when using the same amount of cells in different extractions. The price is reasonable and less expensive compared to what our lab was purchasing before. Add to it, its ease of use, and the short time you spend doing the extractions."

"I love using this kit because it is so easy and I can always rely on the results. I consistently get high RNA yield which helps moving forward into qRT-PCR assays. I've compared this kit to other similar kits and prefer it over others. Thanks Zymo for making a great product!"

"The bennefit of this product is that the amount of sample can be very small, that is very important when you work with a primary cell culture. Also the quality of RNA is very high even when the amount of RNA is not high. RNA isolated using this kit is always good quality. We do regular checks using an Agilent system and the RIN is always better than with other companies' kits."

"This product is very easy to use, quick, the amount of RNA extracted is higher compared to similar products from other manufacturers. Great price/quality ratio."

"I used the Quick-RNA Miniprep Plus in my own research, and thought the instructions so straightforward, and the kit so consistent in results, that I bought several kits for a senior undergraduate lab class that I teach. Each group has their own original individual project, and most of them start with having to clone a gene de novo from a tissue or organism.The DNA/RNA shield meant that I didn't have to worry too much about watching them in the first few stages of the prep, and most of them got the concept of keeping things RNAse-free during the latter stages. Every group got excellent total RNA (the RNA gels looked wonderful) and were able to make cDNA and preform successful PCR. So many possible. teaching moments - who knew that different organisms had slightly different sizes os 18s and 28s RNAs? This class does now."

"We switched from the Q company to Zymo And couldn't be happier. Before we switched we performed extensive testing to make sure that we were not going to lose quality in our RNA. The RNA isolated from this kit was actually better than our previous kits. The time to go through the protocol is about the same, maybe a little longer because the DNase is included in this kit (I previously did this step prior to my RT step). So overall no major time difference. The columns and the collection tubes are packaged separately so you have to put them together yourself as opposed to have them pre-assembled in the Q company's kit."

"This product works very well on tough, waxy leaves of tropical rain forest plant species. I have even compared the effectiveness of the DNA/RNA Shield versus other products meant to reduce RNA degradation (i.e. RNALater) and found it to be more effective at maintaining non-degraded RNA. In my opinion, there is no product on the market that is as good a value and as effective as this kit for plant tissue RNA extractions."

"There is everything inside the Kit. It's easy and really fast compare to Tri reagent protocol "

"High quality RNA was obtained. The product was very easy to use, and no genomic DNA contamination was detected."