Status:
ReviewerMember since: 2022
Organization: uthealth
Great results can't live without this instrument!
Application Area: RNA sequencing
"We have been using the ZYMO RNA Miniprep for many years. with its high quality and effectiveness, our experiments get done much easier. We just ordered 5 packages from them last Friday, and we received it on Monday, its delivery is quick and no hassle. I gave 5 stars on ease of use, after sale care and value for money. We are satisfied with the products from ZYMO research. Glad to collaborate with them!"
Status:
ReviewerMember since: 2022
Organization: University of Bristol
Great results!
Application Area:Extracting RNA from cells
"It works extremely well. Fast and easy to use. You always get a very big amount of RNA and it is of high quality too"
Status:
Advanced ReviewerMember since: 2022
Organization: Pontificia Universidad Católica de Chile
High integrity RNA
Application Area:RNA extraction
"The Quick-RNA™ Miniprep Plus enables you to obtain high-integrity RNA for various downstream analyses, including RT-PCR to RNA-Seq. Its cost is low compared to others available on the market, and it can be combined with the DNA/RNA Shield sample collection and storage kits."
Status:
ReviewerMember since: 2022
Organization: Aridis Pharmaceuticals, Inc.
It is a great kit.
Application Area:RNA isolation from mammalian cells
"It is easy to use, and the RNA quality after isolation is good."
Status:
Advanced ReviewerMember since: 2021
Organization: the Univeriaty of Alabama in Huntsville
Easy to use and we pay extra for this ease of use
Application Area:Total RNA isolation for NGS
"Easy instructions to follow and we get consistent results among experimenters with different backgrounds."
Status:
ReviewerMember since: 2022
Organization: Virginia Tech
Great RNA quality for PCR analysis.
Application Area:RNA extraction
"Very high and good quality RNA yields in a very short time."
Status:
Advanced ReviewerMember since: 2021
Organization: ASDRP
This kit is fast, innovative, and easy! Would definitely recommend.
Application Area:Isolate RNA from tissue culture
"This kit was extremely good at isolating RNA, and was also very fast. We were able to use it multiple times and get good concentrations of RNA, which we used later. Overall, would recommend!"
Status:
ReviewerMember since: 2021
Organization: Sam Houston State University
Easy to use.
Application Area:Isolate RNA from bacteria
"The RNA isolation kit was easy to use. The protocol was easy to follow and the kit came with everything needed to perform good quality RNA isolation. This kit is perfect for someone (like me) who was just starting out with RNA isolation."
Status:
ReviewerMember since: 2021
Organization: MDxHealth Inc.
Always getting accurate results!
Application Area:Buffer preparation
"We have been using this products for so many years now, and the quality and consistency are still the same."
Status:
ReviewerMember since: 2021
Organization: University of Arizona
Great product, I love it.
Application Area:Cardiovascular studies
"The Mini-RNA kit is quite convenient and work really well. The price is relatively low compared to other brands."
Status:
ReviewerMember since: 2021
Organization: University of California
Cant imagine RNA isolation without this kit!
Application Area:Isolation of RNA from postmortem human brain
"In our lab, we always use Zymo RNA isolation kit while dealing with important samples. Its a real blessing to have this awesome, time saving, highly reproducible kit which always gives high quality RNA."
Status:
ReviewerMember since: 2021
Organization: Mississippi state university
It really is a good product if you are planning to isolate RNA from tissue.
Application Area:RT-PCR
"It is a great kit (R1057) compared with the Miniprep one (R1054). I used both, and this kit helped to remove the protein contamination and the purity was good. To identify the quality, I used Nanodrop to test the A260/A230 and A260/A280, and gel electrophoresis applied, the ratio of 28S/18S was 2:1. Also, some samples were analyzed by 2100 bioanalyzer, the RINs were in 7-8.5. Read the protocol carefully and it easy to follow. One suggestion is to use the RNase AWAY to clean the environment and pipettes before the isolation. Another is that do not use too many samples, I tried to use different amount samples including liver, brain, and testes, less than 50 mg was good enough, more samples may result in less production. It is a good idea if you used DNA/RNA shield (or RNA latter) to store samples, if not please do not store those samples too long. I choose the Miniprep kit first because of the low price, but the results were not ideal, and I send the result to the Zymo. They helped to analyze the result and made good suggestions and send me samples of the Plus kit for free. It really helped my study. I recommend the plus kit (R1057)."
Status:
ReviewerMember since: 2019
Organization: University of Wisconsin Madison
Excellent RNA quality and consistent quantity.
Application Area:Extract RNA from cultured cells
"I have used many different kits from different suppliers, I have struggled a lot with RNA quality from small amounts of cultured cells when using other kits; however, with this Quick RNA miniprep plus kit, the quality has been excellent! My ratios never fell below 2. The quantity of RNA is more consistent when using the same amount of cells in different extractions. The price is reasonable and less expensive compared to what our lab was purchasing before. Add to it, its ease of use, and the short time you spend doing the extractions."
Status:
ReviewerMember since: 2019
Organization: AMRI
Awesome kit that I can always rely on for my RNA needs!
Application Area:Extract RNA from PBMC samples
"I love using this kit because it is so easy and I can always rely on the results. I consistently get high RNA yield which helps moving forward into qRT-PCR assays. I've compared this kit to other similar kits and prefer it over others. Thanks Zymo for making a great product!"
Status:
ReviewerMember since: 2019
Organization: National cancer institute
It save time, samples, and grants.
Application Area:Separate total RNA from cell lines cultured in 2D and fresh tissue. and from primary cells
"The bennefit of this product is that the amount of sample can be very small, that is very important when you work with a primary cell culture. Also the quality of RNA is very high even when the amount of RNA is not high. RNA isolated using this kit is always good quality. We do regular checks using an Agilent system and the RIN is always better than with other companies' kits."
Status:
ReviewerMember since: 2016
Organization: University of Verona - Dept. Diagnostics and Public Health - section microbiology
Good product for good results.
Application Area:Analyze transcripts after drug treatment
"This product is very easy to use, quick, the amount of RNA extracted is higher compared to similar products from other manufacturers. Great price/quality ratio."
Status:
ReviewerMember since: 2019
Organization: Department of ephemiology, University of Life
A great RNA extraction kit with simple instructions that gives consistent results.
Application Area:Extraction and purification of RNA from tissue
"I used the Quick-RNA Miniprep Plus in my own research, and thought the instructions so straightforward, and the kit so consistent in results, that I bought several kits for a senior undergraduate lab class that I teach. Each group has their own original individual project, and most of them start with having to clone a gene de novo from a tissue or organism.The DNA/RNA shield meant that I didn't have to worry too much about watching them in the first few stages of the prep, and most of them got the concept of keeping things RNAse-free during the latter stages. Every group got excellent total RNA (the RNA gels looked wonderful) and were able to make cDNA and preform successful PCR. So many possible. teaching moments - who knew that different organisms had slightly different sizes os 18s and 28s RNAs? This class does now."
Status:
ReviewerMember since: 2016
Organization: University of Washington
Great quality RNA, better price, includes DNase in kit
Application Area:Gene expression in tissue and cultured cells
"We switched from the Q company to Zymo And couldn't be happier. Before we switched we performed extensive testing to make sure that we were not going to lose quality in our RNA. The RNA isolated from this kit was actually better than our previous kits. The time to go through the protocol is about the same, maybe a little longer because the DNase is included in this kit (I previously did this step prior to my RT step). So overall no major time difference. The columns and the collection tubes are packaged separately so you have to put them together yourself as opposed to have them pre-assembled in the Q company's kit."
Status:
ReviewerMember since: 2016
Organization: Occidental College
Amazing results, the only RNA extraction kit I ever buy now.
Application Area:Plant RNA extractions
"This product works very well on tough, waxy leaves of tropical rain forest plant species. I have even compared the effectiveness of the DNA/RNA Shield versus other products meant to reduce RNA degradation (i.e. RNALater) and found it to be more effective at maintaining non-degraded RNA. In my opinion, there is no product on the market that is as good a value and as effective as this kit for plant tissue RNA extractions."
Status:
ReviewerMember since: 2016
Organization: CEA/Genoscope
Useful
Application Area:Transcriptomic analysis
"There is everything inside the Kit. It's easy and really fast compare to Tri reagent protocol "
Status:
ReviewerMember since: 2016
Organization: MIT
Great product, will definitely continue using this.
Application Area:qRT-PCR for quantifying transcripts
"High quality RNA was obtained. The product was very easy to use, and no genomic DNA contamination was detected."
The Quick-RNA ™ Miniprep kits are innovative product designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 10 7 ) and tissue samples (up to 50 mg) . The procedure combines a unique buffer system with Zymo-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes. The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin ™ Columns . The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc . In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.