Quick-DNA™ Fecal/Soil Microbe Microprep Kit by Zymo Research

Manufacturer Zymo Research  |   Model: D6012  |  Available Worldwide
4.8
/
5.0
  |  3 reviews
Extract high quality, inhibitor-free metagenomic DNA from feces, soil, water, etc.


Quick-DNA™ Fecal/Soil Microbe Microprep Kit by Zymo Research product image
Quick-DNA Fecal/Soil Microbe Microprep Kits
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Average Rating: 4.8
3 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2021

  • Organization: Alexandria Faculty of Medicine



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results.
Rating: 5.0

  • Application Area: Analyze stool for reseach for gut microbiome

"Zymo kits are friendly, easy to use, short time, simple, all reagents are present in the kit, good extraction for stool samples."

Review date: 16 Mar 2021 | Quick-DNA™ Fecal/Soil Microbe Microprep Kit
  • Status:

    Advanced Reviewer

  • Member since: 2019

  • Organization: MicrobiType LLC



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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This kit eliminates two of the most important problems with getting usable DNA preps.
Rating: 4.3

  • Application Area:Bacterial DNA purification from diverse samples containing PCR inhibitors: stool, soil, food etc.

"PCR inhibitors and particulates are common within fecal and soil samples and can complicate downstream use (in my case, direct Sanger sequencing of PCR product). This kit (available in different capacities - I have used both microprep and miniprep kits) is designed to deal with these problems, by incorporating an initial column to remove particulates and a final column to remove PCR inhibitors. I have used this kit to successfully detect and type bacterial pathogens in stool. But beyond that, the kit has proven ideal for other "messy" samples, including food (poultry, etc.) rinses, food enrichments, and environnmental samples (specifically, listeria and c. diff cultures from environmental sampling tests that incorporate agar and other particulate or PCR inhibitor components). Indeed, several comparisons that I made of DNA preps from complete purifications versus purifications without the final inhibitor-removal column have demonstrated the importance of that final step. I routinely use this kit for DNA preps now, because with diverse samples you never know when an inhibitor might rear its ugly head!"

Review date: 30 Nov 2019 | Quick-DNA™ Fecal/Soil Microbe Microprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Biology, Western University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Zymo DNA kits work - the proof is in the PCR.
Rating: 5.0

  • Application Area:Soil microbial metabarcoding

"We have been users of the Zymo Soil DNA kits for years, first the ZR Soil Microbial DNA Microprep kit (D6003), then the Quick-DNA Fecal/Soil Microbe Microprep (D6012), and most recently the ZymoBIOMICS (Soil) DNA Microprep (D4301). All have been easy to use and consistent in their results. Indeed, when a previous PhD student started her work on metabarcoding from peat, I had her do a side-by-side comparison of the Zymo kit with kits from two other manufacturers, some of which were touted as especially designed for high humic acid samples. The DNAs recovered using the Zymo kits were more consistently PCR-amplifiable than those recovered using the other kits."

Review date: 05 Jun 2019 | Quick-DNA™ Fecal/Soil Microbe Microprep Kit
The Quick-DNA™ Fecal/Soil Microbe Kits are designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads™. Zymo-Spin™ column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.

Product Overview

Quick-DNA™ Fecal/Soil Microbe Microprep Kit by Zymo Research product image

Quick-DNA™ Fecal/Soil Microbe Microprep Kit

Manufacturer Zymo Research  |  Available Worldwide

4.8 / 5.0 | 3 reviews