Status:
ReviewerMember since: 2019
Organization: Maimónides Institute for Biomedical Research at Córdoba
Great!
Application Area: Molecular Biology
"It was really easy to use and understand, the protocol is well written and the post service is great."
Status:
ReviewerMember since: 2019
Organization: IMIBIC
Great qPCR kit for gene expresion.
Application Area:Molecular Biology
"qPCR is a technology with high variability. This kist improves the variability in comparison with other ones."
Status:
ReviewerMember since: 2019
Organization: UPCH
Very good product.
Application Area:Molecular Biology
"Very good product. Allows me to get good performance in my experiments with real-time PCR. I fully recommend it. "
Status:
ReviewerMember since: 2019
Organization: Euroeducation
It's very easy to use and it's given me high-quality intact RNA profiling .
Application Area:Molecular biology analysis of microarray experiments of gene expression profiling
"This kit was vital in my work for finding differences in expression levels between predefined groups of samples, that is in my "class comparison" experiment. It's been also a great help for analyzing sets of gene expression profiles with the goal of discovering subgroups that share common features."
Status:
ReviewerMember since: 2019
Organization: UPCH
Very good product, you have to try it NOW
Application Area:Research in Neurocysticercosis
"I have had remarkably good results like no other. I was surprised by the efficiency of the product and the support received by the company. I recommend them totally."
Status:
ReviewerMember since: 2019
Organization: Cordoba University
Formidable product
Application Area:Molecular biology
"It´s an easy to use product to obtain high quality results in gene expression experiments"
Status:
ReviewerMember since: 2019
Organization: REDOX LAB S.L.
Best value in the market
Application Area:Investigation area
"The product is high quality, easy to use and has a magnificent price "
Status:
ReviewerMember since: 2019
Organization: Aristotle University Of Thessaloniki
I do not need to replace it with another similar kit.
Application Area:Veterinary research
"is a product that yielded very satisfactory results, with great precision and repeatability. it has a very low cost and there is an immediate response to the order."
Status:
ReviewerMember since: 2019
Organization: Cordoba University
Good product
Application Area:Molecular biology
"It is easy and ready to use for expression analysis"
Status:
ReviewerMember since: 2019
Organization: Anopoli
Very valuable mastermix, greater specificity, sensitivity & yields in a single tube.
Application Area:Dual probe RT qPCR assay for the detection of Mers-Corona Virus
"We could generate high quality results it is very flexible, easy to use, and short hands on time. We will recommend it to research groups working in the same field."
Status:
ReviewerMember since: 2019
Organization: I+D
Great performance.
Application Area:PCR Analysis
"Easy to use and accurate high quality results. Used for simultaneously detection and quantification of DNA or RNA. Really optimized master mix."
Status:
ReviewerMember since: 2019
Organization: University Of Veterinary And Animal Sciences Lahore
This products helps me alot in my research work.
Application Area:I am using this product in the detection of Foot and Mouth Disease Virus
"I am using this product in my research work for the detection of Foot and Mouth Disease Virus. These kits are very effective and give accurate, high quality results. I recommend it to others."
Status:
ReviewerMember since: 2008
Organization: University of Texas health science center
It is an essential product in the lab for cDNA synthesis and saves time.
Application Area:cDNA synthesis for QPCR
"Greater specificity, sensitivity & yields in cDNA synthesis and qPCR in a single tube."
Status:
ReviewerMember since: 2019
Organization: IMIBIC
As good as kits that are twice the price.
Application Area:Detection and quantification of DNA and cDNA targets
"Results are equally good at almost half the price. We have been using it a lot in the past few months and we will not be switching back to the competition."
qMAXSen™ One-Step Green RT-qPCR Kit (Low Rox™) offers greater specificity, sensitivity, and yields in cDNA Synthesis and qPCR in a single tube. The kit includes a qPCR MasterMix supplied in a 2x concentration to perform Real-Time PCR using a proprietary green dye. The qPCR MasterMix contains all the reagent (except PCR Primers and Template) needed for running PCR reactions. In addition, a separate RT mix that comprises a balanced mixture of both RTase and RNase Inhibitor is also supplied.
Available with the option of ROX™ as the internal passive reference dye. The ROX™ dye provides an internal reference to which the reporter-dye signal can be normalized during data analysis.
RT-PCR is used to amplify double-stranded DNA from single-stranded RNA templates. In the RT step the Reverse Transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step synthesis, Taq DNA polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. During subsequent rounds of cycling the DNA polymerase exponentially amplifies the double-stranded DNA template.