Q5® High-Fidelity DNA Polymerase by New England Biolabs Inc.

4.5
/
5.0
  |  5 reviews
Q5® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate > 100-fold lower than that of Taq DNA Polymerase and 12-fold lower than that of Pyrococcus furiosus (Pfu) DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons.Q5 High-Fidelity DNA Polymerase is supplied with an optimi... Read more


Q5® High-Fidelity DNA Polymerase by New England Biolabs Inc. product image
Q5® High-Fidelity DNA Polymerase

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Average Rating: 4.5
5 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2021

  • Organization: Miami University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product!
Rating: 5.0

  • Application Area: DNA cloning

"I always achieve high quality and reproducible results using this product."

Review date: 17 Apr 2021 | Q5® High-Fidelity DNA Polymerase
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Unviersity of Chicago



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Efficient PCR amplification for difficult templates.
Rating: 4.7

  • Application Area:PCR for recombinant DNA synthesis

"The formulation that we purchased is the High Fidelity Hot Start Master Mix. Remarkably simple to use given the formulation without the need to pipette individual components. Accounting for the slightly higher annealing temperature needed for Q5, we were able to obtain several PCR products that were not amplified with our other favorite proofreading polymerase. It seemed to work particularly well with some of our genomic templates. Overall a great product."

Review date: 17 Jun 2020 | Q5® High-Fidelity DNA Polymerase
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: University of Montreal



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
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Reliable!
Rating: 4.0

  • Application Area:Molecular biology

"Reliable and fast. Price is not competitive."

Review date: 26 Feb 2020 | Q5® High-Fidelity DNA Polymerase
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: University Hospital Southampton NHS Trust



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great Reagent
Rating: 5.0

  • Application Area:NGS amplicon library preparation

"Great easy to use reliable Master mix. A proof reader with 3-5' exonuclease activity, great amplification of many targets but can permit binding and amplification to pseudogenes so careful with your designs."

Review date: 01 Nov 2017 | Q5® High-Fidelity DNA Polymerase
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Columbia University



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great!
Rating: 4.0

  • Application Area:Cloning, next gen library prep

"The 2x mastermix makes any PCR set up super quick."

Review date: 12 Jul 2016 | Q5® High-Fidelity DNA Polymerase
Q5® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification.

With an error rate > 100-fold lower than that of Taq DNA Polymerase and 12-fold lower than that of Pyrococcus furiosus (Pfu) DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons.

Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM MgCl2 at final (1X) reaction concentrations and is recommended for most routine applications.

For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.


Q5® High-Fidelity DNA Polymerase Applications:

  • High-fidelity PCR
  • Cloning
  • Long or Difficult Amplification
  • High-throughput PCR