Member since: 2021
Organization: Miami University
Application Area: DNA cloning
"I always achieve high quality and reproducible results using this product."
Member since: 2019
Organization: Unviersity of Chicago
Efficient PCR amplification for difficult templates.
Application Area:PCR for recombinant DNA synthesis
"The formulation that we purchased is the High Fidelity Hot Start Master Mix. Remarkably simple to use given the formulation without the need to pipette individual components. Accounting for the slightly higher annealing temperature needed for Q5, we were able to obtain several PCR products that were not amplified with our other favorite proofreading polymerase. It seemed to work particularly well with some of our genomic templates. Overall a great product."
Member since: 2017
Organization: University Hospital Southampton NHS Trust
Application Area:NGS amplicon library preparation
"Great easy to use reliable Master mix. A proof reader with 3-5' exonuclease activity, great amplification of many targets but can permit binding and amplification to pseudogenes so careful with your designs."
With an error rate > 100-fold lower than that of Taq DNA Polymerase and 12-fold lower than that of Pyrococcus furiosus (Pfu) DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons.
Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM MgCl2 at final (1X) reaction concentrations and is recommended for most routine applications.
For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.
Q5® High-Fidelity DNA Polymerase Applications: