Q5® High-Fidelity 2X Master Mix by New England Biolabs Inc.

4.9
/
5.0
  |  4 reviews
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance. Working with uracil-containing DNA templates or using dUTP? Learn about Q5U ... Read more


Q5® High-Fidelity 2X Master Mix by New England Biolabs Inc. product image
Q5® High-Fidelity 2X Master Mix

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Average Rating: 4.9
4 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2022

  • Organization: BOKU



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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The Q5 master mix: Cost-effective product for any PCR application
Rating: 4.7

  • Application Area: Reliable polymerase suitable for any PCR

"Q5 is a robust polymerase giving reproducible results using different PCR methods. The polymerase batches stay active beyond expiration date and the suggested reaction volumes can be significantly reduced (10μL) to save reagents. Moreover, the mastermix reduces pitpetting steps, thus the variability between reactions while saving again material."

Review date: 18 Nov 2022 | Q5® High-Fidelity 2X Master Mix
  • Status:

    Advanced Reviewer

  • Member since: 2021

  • Organization: MiNK Therapeutics



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great overall HiFi polymerase
Rating: 5.0

  • Application Area:Routine HiFi PCR

"Very robust HiFi polymerase, we use it daily in all our workflows that involve PCR, using a variety of inputs ranging from crude lysates, to gDNA and plasmid DNA."

Review date: 18 Nov 2022 | Q5® High-Fidelity 2X Master Mix
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Folium



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great experience and working very well
Rating: 5.0

  • Application Area:DNA amplification of highly complex gene

"Achieve my expectation of results in comparison with other polymerases"

Review date: 29 Mar 2022 | Q5® High-Fidelity 2X Master Mix
  • Status:

    Advanced Reviewer

  • Member since: 2020

  • Organization: Dartmouth College



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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High fidelity and reliable DNA polymerase.
Rating: 5.0

  • Application Area:PCR, DNA polymerase

"Q5 master mix is a DNA polymerase which is very accurate and much faster than Taq with proofreading ability."

Review date: 17 Jun 2020 | Q5® High-Fidelity 2X Master Mix

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Hot start and master mix formats available