Status:
ReviewerMember since: 2022
Organization: BOKU
The Q5 master mix: Cost-effective product for any PCR application
Application Area: Reliable polymerase suitable for any PCR
"Q5 is a robust polymerase giving reproducible results using different PCR methods. The polymerase batches stay active beyond expiration date and the suggested reaction volumes can be significantly reduced (10μL) to save reagents. Moreover, the mastermix reduces pitpetting steps, thus the variability between reactions while saving again material."
Status:
Advanced ReviewerMember since: 2021
Organization: MiNK Therapeutics
Great overall HiFi polymerase
Application Area:Routine HiFi PCR
"Very robust HiFi polymerase, we use it daily in all our workflows that involve PCR, using a variety of inputs ranging from crude lysates, to gDNA and plasmid DNA."
Status:
ReviewerMember since: 2020
Organization: Folium
Great experience and working very well
Application Area:DNA amplification of highly complex gene
"Achieve my expectation of results in comparison with other polymerases"
Status:
Advanced ReviewerMember since: 2020
Organization: Dartmouth College
High fidelity and reliable DNA polymerase.
Application Area:PCR, DNA polymerase
"Q5 master mix is a DNA polymerase which is very accurate and much faster than Taq with proofreading ability."
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).