Proteinase K (PK) Solution by Promega Corp.
Status:
Reviewer
Member since: 2021
Organization: MNCN-CSIC
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- Ease of use
- After sales service
- Value for money
Very good results
Application Area: DNA extraction
"I have been working with this product for many years and it always gives very good results, I would not change it for any other. It may cost a little more but it is worth it."
Review date: 08 Jun 2022 | Proteinase K (PK) Solution
- Active over pH range 4.3–12.0 in 0.5% SDS or 1% Triton® X-100
- Retains >80% activity at temperatures up to 60°C
- No resuspension or thawing before use
- Cat.# MC5005, MC5008 supplied at a concentration of 20mg/ml
Proteinase K, produced by the fungus Tritirachium album Limber, is a serine protease that exhibits broad cleavage activity. It cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids and is useful for general digestion of protein in biological samples. It has been purified to remove RNase and DNase activities. The stability of Proteinase K in urea and SDS and its ability to digest native proteins make it useful for a variety of applications including preparation of chromosomal DNA for pulsed-field gel electrophoresis, protein fingerprinting and removal of nucleases from preparations of DNA and RNA. A typical working concentration for Proteinase K is 50–100μg/ml.
Formulation: Proteinase K (PK) Solution is supplied at a concentration of 20mg/ml in 10mM Tris-HCl (pH 7.5), 1mM calcium chloride and 50% glycerol.
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