PrimePCR™ PCR Primers, Assays, and Arrays
Experimentally validated PCR primer and probe assays for gene expression, copy number variation, and mutation detection analysis for real-time PCR and Droplet Digital PCR.
PrimePCR PCR Primers, Assays & Arrays
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Highly useful equipment for a lab working on mutational analysis
Mutation analysis
Highly sensitive method and reproducible assays. It provides absolute quantification of copy number without the use of external calibrator.
Review Date: 9 Apr 2018 | Bio-Rad
PCR Primer and Probe Assays for Real-Time PCR
Our real-time PCR primers were designed in collaboration with leading experts in real-time PCR research. Every PCR primer pair has been experimentally validated to ensure optimal assay performance.
Our fluorescently labeled oligonucleotide probes were designed to enable multiplexing. These 5' nuclease probes were designed for maximum specificity and transcript coverage. qPCR probe assays are available with a FAM, HEX, TEX615, Cy5, or Cy5.5 fluorophore.
Real-time PCR assays are available in the following formats:
- Predesigned primer and probe assays — gene expression assays for the human and mouse genomes
- Custom oligonucleotides — design your own PCR primer or probe assay
- Customizable 96- and 384-well plates — use the custom plate configurator to conveniently design a PCR plate
- Predesigned PCR Arrays — select from a wide variety of disease- and pathway-specific arrays
- PreAmp assays — 1,000-fold unbiased target-specific preamplification of limited nucleic acid
Controls and Reference Assays are available to facilitate assay normalization and control:
- Experimental Controls — for evaluating reverse transcription performance, RNA quality, genomic DNA contamination, and PCR reaction performance
- Reference Gene Assays — for relative gene expression analysis to normalize for variation in the amount of input messenger RNA (mRNA) between samples
- DNA templates — serve as a positive real-time PCR control for the corresponding gene assay
All real-time PCR assays and arrays can be used with CFX real-time PCR detection systems and data analysis software, streamlining PCR data collection and analysis.
Probe Assays for Droplet Digital PCR
Droplet Digital PCR (ddPCR™) technology provides an absolute measure of target DNA molecules with unrivaled accuracy, precision, and sensitivity, accelerating discovery and enabling new research strategies for inherited disorders, cancer, and infectious disease.Probe assays for the QX200™ Droplet Digital PCR system enable the fine quantitative discrimination required to resolve small fold changes without a standard curve. ddPCR probe assays are available with a FAM or HEX fluorophore.
ddPCR assays are available in the following formats:
- Predesigned mutation detection probe assays — for human samples
- Predesigned copy number variation (CNV) probe assays — for human samples
- Custom oligonucleotides — design your own ddPCR probe assay
PrimePCR assays, plates, and controls have a guaranteed shelf life of 12 months when stored at 4°C.
PrimePCR™ Analysis Software
Our custom data analysis software enables gene expression analysis of PrimePCR data generated on any real-time PCR instrument. Analysis options include bar chart, clustergram, scatter plot, volcano plot, and heat map analysis.
Key considerations in qPCR assay controls
Quantitative PCR (qPCR) is a widely utilized technique for the semi-quantitative study of gene expression. However, its accuracy and reproducibility depend significantly on the proper use of assay controls.
This eBook explores the distinct types of assay controls crucial for reliable qPCR results and provides guidance on the design and application of these controls, including the DNA contamination control, positive PCR control, RNA quality assessment, reverse transcription control, and on the selection and validation of reference genes with the use of prevalidated assays.
Multiplex Screening of KRAS Mutations in Cell-Free DNA Colorectal Cancer Samples
This application note describes a ddPCR KRAS Screening Multiplex Kit for screening actionable KRAS mutations using DeopletDigital PCR (ddPCR). The ability to screen for multiple KRAS mutations down to 0.2% in a single well reduces the likelihood that mutations will be missed in poor-quality cell-free DNA anf formalin-fixed, paraffin-embedded (FFPE) samples with limited amounts of amplifiable DNA.
Protocol for Single Cell Gene Expression Profiling by Multiplex RT-qPCR
Single cell gene expression analysis is a powerful technique that provides a unique and insightful perspective on biological pathways and processes. This application note describes a validated protocol that enables fast and accurate analysis of up to 100 genes in isolated single cells, using multiplex reverse transcription quantitative PCR (RT-qPCR).
Accelerating Cancer Genetics Research Using Digital PCR
Professor of Genetics, Diptasri Mandal and graduate student, Kirsten Wood, of the Department of Genetics at Louisiana State University Health Sciences Center, explain how custom digital PCR assays are facilitating their research into hereditary biomarkers of prostate cancer.
New Method to Assess Effectiveness of Genome-Editing Technologies
Droplet Digital™ PCR–based assay quickly and cost-effectively assesses genome-editing events
Risk Factors for Prostate Cancer Identified and Validated Using Droplet Digital PCR
Learn how digital PCR is advancing cancer genetics research at Louisiana State University Health Sciences Center
