Precision Plus Protein™ WesternC™ Blotting Standards, 250 µl by Bio-Rad

Manufacturer Bio-Rad  |   Model: 1610376
250 µl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications


Precision Plus Protein™ WesternC™ Blotting Standards, 250 µl by Bio-Rad product image
Precision Plus Protein™ WesternC™ Blotting Standards, 250 µl
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Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. These standards contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development.

Applications and Uses

  • Monitoring electrophoretic separation
  • Monitoring blot transfer efficiency with easy orientation via multicolor standards
  • Molecular weight sizing of proteins after blot development
  • Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range

More Information

Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN3, 0.01% bromophenol blue.

Fluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. No conjugate is needed for fluorescent blot detection.

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More Precision Plus Protein Standards:

  • Precision Plus Protein Unstained Standards
  • Precision Plus Protein Prestained Standards
  • IEF and 2-D Electrophoresis Protein Standards

Related Categories

  • Protein Ladders and Standards
  • Precast Protein Gels
  • Protein Electrophoresis and Blotting
  • Western Blotting

Online Resources

  • Protein Electrophoresis
  • Western Blotting