Precision Plus Protein™ WesternC™ Blotting Standards, 250 µl
250 µl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications
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Live with instrument for routine analysis of protein in lab
Efficient separation of protein
High proficient reproducible protein separation. Due to the range of separation limit the reduced interference and increased accuracy of the techniques. Value for the money.
Review Date: 19 Nov 2022 | Bio-Rad
Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. These standards contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development.
Applications and Uses
- Monitoring electrophoretic separation
- Monitoring blot transfer efficiency with easy orientation via multicolor standards
- Molecular weight sizing of proteins after blot development
- Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range
More Information
Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN3, 0.01% bromophenol blue.
Fluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. No conjugate is needed for fluorescent blot detection.
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More Precision Plus Protein Standards:
- Precision Plus Protein Unstained Standards
- Precision Plus Protein Prestained Standards
- IEF and 2-D Electrophoresis Protein Standards
Related Categories
- Protein Ladders and Standards
- Precast Protein Gels
- Protein Electrophoresis and Blotting
- Western Blotting
Online Resources
- Protein Electrophoresis
- Western Blotting
