Overnight Express Instant TB Medium is a complete granulated culture medium for high-level protein production without the need to monitor cell growth in the pET and other IPTG-inducible bacterial expression systems. Cell mass and target protein yield are often increased several-fold as compared with induction using IPTG.
The method is based on media components that are metabolized differentially to promote growth to high density and automatically induce protein expression from lac promoters.
The Instant Medium is supplied in two formats: EasyPak is an aluminum foil pouch containing 60 g granulated medium sufficient for 1 L culture. Just add the EasyPak contents to 1 L water, supplement with 10 ml glycerol, and microwave for 2 minutes to dissolve.
In addition, the Overnight Express Instant TB Medium is supplied in 1 kg bottles. The granules ensure rapid and uniform dissolution in water and prevent clumping of the medium and inhalation of the airborne powder.