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Member since: 2018
Application Area: realtime intereaction of bio molecules.
"Octet is very useful for High Throughput Screening screening of antibodies and mutants. very useful for antibody engineering."
Member since: 2017
Organization: University of Cambridge
Has become the most popular instrument in our busy biophysics facility.
Application Area:Protein-protein interactions
"Very easy and intuitive to use and relatively low cost of biosensors allows pilot work to be undertaken without a huge initial financial commitment, which is important in an academic (i.e. grant-funded) setting. Good choice of biosensors, and is increasing over time, although the manufacturers could provide more details about surface chemistry (e.g. capture sensors that are in fact biotin-streptavidin based). Evaporation on the plate has been addressed by a possible instrument upgrade. Would like to see an option for half-well/lower volume plates. Software upgrades are expensive!"
Member since: 2017
Great instrument, easy to use!
Application Area:Protein protein interaction
"Octet Red96 is a very nice piece of equipment. It is great for measuring protein interactions in a high-throughput manner. The affinities obtained are equivalent to what can be measured by other techniques, such as ITC, SPR, MST and others. The equipment can also be used for protein quantification, and can be used in "dirty" samples, such as extracts, as it doesn't use any microfluidics."
Member since: 2014
Organization: Fox Chase Cancer Center
"It's an easy going and user friendly instrument. You can get high quality results with lowest amount of protein sample."
Member since: 2013
Organization: XBiotech USA Inc.
The Octet RED is an excellent instrument for fast quantification and initial screens of antibody binding kinetics.
"The Octet RED is an excellent instrument for fast quantification and initial screens of antibody binding kinetics. While it may not give the same resolution that is achieved through traditional SPR techniques, the Fortebio Octet RED system is able to produce reliable, accurate and repeatable results at a much higher throughput and a greatly reduced consumables cost. It is absolutely recommended for any application that requires routine kinetics screening of multiple protein binding partners or binding conditions. "
Member since: 2010
"The system works well."
The 8-channel system - Octet RED96e - offers an advanced fluidics-free approach reducing assay run time and minimizing maintenance efforts and cost. The system utilizes Bio-Layer Interferometry (BLI) technology, enabling direct detection of specific proteins and other biomolecules — even in complex mixtures like cell culture supernatants and lysates.
Popular industrial applications include expression screening, on- and off-rate screening, epitope binning, cell line development and bioprocess optimization. The Octet RED96e system is widely used in analytical core labs for protein structure/function studies and small molecule screening campaigns. The system has been developed to operate reliably in a regulated environment. Pall ForteBio offers 21 CFR Part 11 software and a full line of GxP products and services as part of the Octet RED96e GxP Package.