Status:
ReviewerMember since: 2020
Organization: Ferring Pharmaceuticals
Would buy that instrument again.
Application Area: IgG Quantitation in Cell Line Development
"Easy to use instrument, good results. Easy to explain to new users. Great support."
Status:
ReviewerMember since: 2021
Organization: Jawaharlal Nehru University
Superb instrument, ideal for high throughput work!
Application Area:Protein-ligand and Protein-protein interaction, fragment based drug development
"The equipment is very easy to operate and great user interface. Moreover, we have achieved great results in our experiments. In fact, we need a very less quantity of protein/ligand to quantitate. It is extremely fast and can perform high throughput experiments easily. Great for screening purposes."
Status:
ReviewerMember since: 2019
Organization: Tampere University
First choice for interaction studies.
Application Area:Drug discovery
"Octet is very easy to use system for fast screening of protein - small molecule interaction studies. Easy to maintain and definitely value for the money because of time saved in measurements time."
Status:
ReviewerMember since: 2022
Organization: Kashvi Life Sciences
Using BLI technology - Octet R series has no match
Application Area:Drug discovery
"Octet R series is fast, easy to use & software is very friendly. Results can be obtained in 3 - 4 hrs run time, even with low volume samples. I strongly recommend as it is very economical , compared to other players in market. Efficient sales & service team to serve customers. Prompt service by team Sartorius. "
Status:
ReviewerMember since: 2021
Organization: Florey Institute of Neuroscience
Powerhouse biomolecular equipment.
Application Area:Protein ligand binding and antibody kinetics
"A powerful addition to our lab. The striking feature that comes up time and again is that the sample is preserved so the ability to control experiments and analyse samples by other techniques is a huge advantage over injection-based flow systems. I can run multiple antibodies through the very same sample and be certain that the result is real and not just ruled out as an anomaly or error."
Status:
ReviewerMember since: 2021
Organization: Sterogene Bioseparations Inc
A must have instrument for drug/antibody discovery and vaccine research labs.
Application Area:Antibody discovery, discovery and clinical
"Octet is one of the best system for determination of binding constants, antibody discovery and vaccine research. It is versatile with various chemistries for ligand immobilization. High throughput, robust, easy to use and excellent technical support."
Status:
Advanced ReviewerMember since: 2020
Organization: Precisionantibody
It is a great tool to have in every antibody development group.
Application Area:Affinity measurements, quantification etc.
"The BLI platform is a great tool, to downsize and pick top ranked potential top candidates. Depending on your end application the uses are many, you can rank antibodies, find potential pairs for sandwich application, quant analysis, Accurate KD measurements, inhibition assays, serum interference and many more. The machine is user friendly, very flexible and a powerful tool."
Status:
ReviewerMember since: 2018
Organization: UPENN
Need consistency.
Application Area:Antibody engineering
"Its worked well for the 1st year, but there is huge variation in the biosensors. Control reactions won't work for months, even application specialists have no idea what is going on."
Status:
ReviewerMember since: 2019
Organization: CSIR IICB
Great product and very useful for drug discovery.
Application Area:Protein-protein, protein-drug interaction studies
"Instrument performance is good. With 8 channels, we are getting results within very short time frames when compared to similar equipment. Software is also very user friendly, all the details are available in a report file. Sensor prices are reasonable and less expensive. No sample loss especially for ordered protein, DNA and other samples. Some sensor type are reusable with protein having histidine tag, GST tag etc.. Small molecules detection is also good for protein-drug, DNA-drug interaction studies. Overall product is good."
Status:
ReviewerMember since: 2019
Organization: CSIR IICB
Good results
Application Area:Protein-protein interactions
"Good and fast for kinetic studies, protein-protein interaction studies, and protein-drug interaction studies."
Status:
ReviewerMember since: 2018
Organization: UPENN
Great instrument
Application Area:realtime intereaction of bio molecules.
"Octet is very useful for High Throughput Screening screening of antibodies and mutants. very useful for antibody engineering."
Status:
ReviewerMember since: 2017
Organization: University of Cambridge
Has become the most popular instrument in our busy biophysics facility.
Application Area:Protein-protein interactions
"Very easy and intuitive to use and relatively low cost of biosensors allows pilot work to be undertaken without a huge initial financial commitment, which is important in an academic (i.e. grant-funded) setting. Good choice of biosensors, and is increasing over time, although the manufacturers could provide more details about surface chemistry (e.g. capture sensors that are in fact biotin-streptavidin based). Evaporation on the plate has been addressed by a possible instrument upgrade. Would like to see an option for half-well/lower volume plates. Software upgrades are expensive!"
Status:
ReviewerMember since: 2017
Organization: Biocant
Great instrument, easy to use!
Application Area:Protein protein interaction
"Octet Red96 is a very nice piece of equipment. It is great for measuring protein interactions in a high-throughput manner. The affinities obtained are equivalent to what can be measured by other techniques, such as ITC, SPR, MST and others. The equipment can also be used for protein quantification, and can be used in "dirty" samples, such as extracts, as it doesn't use any microfluidics."
Status:
ReviewerMember since: 2014
Organization: Fox Chase Cancer Center
Highly reliable
Application Area:Biochemistry
"It's an easy going and user friendly instrument. You can get high quality results with lowest amount of protein sample."
Status:
Advanced ReviewerMember since: 2013
Organization: XBiotech USA Inc.
The Octet RED is an excellent instrument for fast quantification and initial screens of antibody binding kinetics.
Application Area:Biotechnology
"The Octet RED is an excellent instrument for fast quantification and initial screens of antibody binding kinetics. While it may not give the same resolution that is achieved through traditional SPR techniques, the Fortebio Octet RED system is able to produce reliable, accurate and repeatable results at a much higher throughput and a greatly reduced consumables cost. It is absolutely recommended for any application that requires routine kinetics screening of multiple protein binding partners or binding conditions. "
The 8-channel system - Octet® R8 - offers an advanced fluidics-free approach to protein characterization. The independent biosensors allow analysis of single or up to eight channels, enabling flexibility in sample throughput based on need. The Octet system performs quantitation of 96 samples in 32 minutes and kinetic screening of 64 samples in 1.5 hours.
The system utilizes Bio-Layer Interferometry (BLI) technology. The real-time technique enables direct detection of specific proteins and other biomolecules — even in complex mixtures like cell culture supernatants and lysates and low concentration environments.
Furthermore, the label-free technology gathers physiologically relevant data quickly, proving to be a cost-effective method.
Key Benefits:
Popular industrial applications include expression screening, on- and off-rate screening, epitope binning, cell line development and bioprocess optimization.
The Octet® R8 system is widely used in analytical core labs for protein structure/function studies and small molecule screening campaigns. The system has been developed to operate reliably in a regulated environment. Sartorius offers 21 CFR Part 11 software and a full line of GxP products and services as part of the Octet® R8 GxP Package.