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Nucleofector® II Device by Lonza Group Ltd

Manufacturer Lonza Group Ltd
4.2
/
5.0
  |  5 reviews
The basic Nucleofector® II Device is a single cuvette based system that has been used in research labs since 2001. It allows efficient transfection of hard-to-transfect cell lines and primary cells with different substrates, e.g. DNA vectors or siRNA oligonucleotides, in low throughput format. What benefits are important for your work? Efficient transfection in single cuvette format? Up to 90% efficiency with plasmid DNA Up to 99% efficiency with siRNA duplexes Also suited for peptides... Read more


Nucleofector® II Device by Lonza Group Ltd product image
Nucleofector® II Device

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Average Rating: 4.2
5 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
Write your own review


  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Incyte



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great system for nuceofecting!
Rating: 4.7

  • Application Area: Transfection

"This system works exactly as intended. Nucleofections are easier to perform as well as scalable to low and high throughput. Great service and wide selection of protocols available with minimal troubleshooting."

Review date: 06 Feb 2019 | Nucleofector® II Device

  • Status:

    Advanced Reviewer

  • Member since: 2015

  • Organization: University of Manchester



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great method for getting constructs and siRNA into cell lines
Rating: 4.7

  • Application Area:Transfection of cell lines

"This is a great method for very high efficiency of cultured cell lines. It's simple to use, very highly reproducible."

Review date: 09 Sep 2016 | Nucleofector® II Device

  • Status:

    Reviewer

  • Member since: 2013

  • Organization: University Of British Columbia



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Rating: 4.0

  • Application Area:Nucleofection Of Primary Cortical Neurones (E16)

"I use the lonza kits (cuvettes) and provided buffer. On the day of plating I get over 80% nucleofection in general and minimal cell death. I've used this product to nucleofect primary cortical, striatal, hippocampal and mixed cultures with great success. "

Review date: 29 Nov 2013 | Nucleofector® II Device

  • Status:

    Reviewer

  • Member since: 2012

  • Organization: National Chiao Tung Univ



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Rating: 3.7

  • Application Area:mice hippocampal neuron

"I used this equipment to study the mophology of neuronal cytoskelon in cell line induced neurons and primary culture hippocampal neurons and cortical neurons."

Review date: 16 Dec 2012 | Nucleofector® II Device

  • Status:

    Reviewer

  • Member since: 2011

  • Organization: UNIVERSITY OF UTAH



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Rating: 3.7
"There are new versions available, but we are concerned about reproducibility if we switch."

Review date: 28 Jun 2011 | Nucleofector® II Device

The basic Nucleofector® II Device is a single cuvette based system that has been used in research labs since 2001. It allows efficient transfection of hard-to-transfect cell lines and primary cells with different substrates, e.g. DNA vectors or siRNA oligonucleotides, in low throughput format.

What benefits are important for your work?

  • Efficient transfection in single cuvette format?
  • Up to 90% efficiency with plasmid DNA
  • Up to 99% efficiency with siRNA duplexes
  • Also suited for peptides, proteins or small molecules

Reliable easy-to-use technology?

  • More than 150 ready-to-use Amaxa® Optimized Protocols
  • High viability and preservation of cell funcionality
  • Data for more than 650 cell types in our Cell Database
  • Consumables tailored to your needs?
  • Nucleofector® Kits are available in low (10 reactions), normal (25 reactions) and high (100 reactions) usage format
  • More than 50 dedicated primary cell kits available
  • A collection of 5 cell line kits and an optimization kit covering the broadest range of cell lines
    cGMP kits available