NTA fast flow histrap column by Cytiva

Manufacturer Cytiva  |  Available Worldwide
4.3
/
5.0
  |  1 reviews
HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).


NTA fast flow histrap column by Cytiva product image
NTA fast flow histrap column
Request Pricing

Receive your quote directly from the manufacturer.




Average Rating: 4.3
1 Scientist has reviewed this product

5 out of 5
Ease of use
3 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Advanced Reviewer
  • Member since: 2019

  • Organization: University of Colorado, School of Medicine, USA



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
Share this Review on LinkedinShare this Review on TwitterShare this Review on Facebook
My go-to column for purification of proteins
Rating: 4.3

  • Application Area: biomolecules purification

"I'm always having his-tagged protein to purify and this is my go-to column. As the column seems to be losing the blue/Ni color quite fast, it's always important to strip and recharge. The column comes with clear instructions on using and recharging."

Review date: 18 Jun 2021 | NTA fast flow histrap column

HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

High binding capacity, approx. 40 mg/mL resin.
Negligible leakage of Ni2+.
Prepacked columns offer reliable and convenient time-saving purification of histidine-tagged recombinant proteins.
Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications

The high flow rates made possible by the Ni Sepharose 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap IMAC FF is the product of choice when charging the resin with different metal ions for optimization of purification protocols.

Product Overview

NTA fast flow histrap column by Cytiva product image

NTA fast flow histrap column

Manufacturer Cytiva  |  Available Worldwide

4.3 / 5.0 | 1 reviews