NovoCyte Quanteon Flow Cytometer by Agilent Technologies

"Tool frequently breaks"

"We had a chance to try out this instrument as part of our workshop on cell tracking and cell functional assays at the 42nd and 43rd Annual Courses in Research Applications of Cytometry (Bowdoin and Madison). Flexibility of optical configuration, wide dynamic range, and ability to independently adjust settings of up to 4 different detectors in a shared spectral window were perfect for applications in which we needed to match multicolor immunofluorescence (including several low-expression antigens) with extremely bright proliferation dyes to analyze differential proliferative responses based on dye dilution. Technical support personnel were extremely knowledgeable and helpful before, during and after the workshop."

"We have had the Quanteon for more than 7 months now. Daily maintenance is straightforward and easy, passing the validation every day with no drama! Users found the software easy to learn and to set up new experiments. Change between the tube mode and plate mode is simply done by choosing the right button within the software, so no tricky fiddling for loading station or sample line. The instrument was ready to use after weeks of COVID-19 lockdown, after the simple routine startup, with no extra clean or alignment, which is impressive!"

"We have had a NovoCyte 3000 for a couple of years and it is a high quality, solid instrument and ACEA’s customer support is among the best I’ve seen in the industry. Therefore, when we had the opportunity to get a more advanced instrument, the Quanteon naturally came to mind. There are several advantages to the Quanteon over the NovoCyte 3000. 1. While the NovoCyte has eight shared PMTs theoretically allowing for up to 13 colors, the Quanteon has 25 independent Silicon Photo Multipliers (each SiPM is essentially an array of APDs acting in unison) which are more sensitive than PMTs. Independent detectors add flexibility for more advanced users. 2. The NovoCyte has 3 lasers, with the Quanteon having 4 more powerful lasers which both adds to sensitivity and ease of panel design. 3. The NovoCyte has 0.2 μm. resolution, the Quanteon pushes this down to 0.1 μm. The greatest thing about the Quanteon, is that if you already know how to use the NovoCyte, the software is the same user-friendly and powerful NovoExpress that you are accustomed to. I have trained 3 undergraduates (one a second semester freshman) to independently operate the Quanteon in the first few months we owned it. As far as customer service, everyone I’ve encountered at ACEA is top drawer. Excellent. I once observed one of their team members, Heather Paich, “triple hat” as a salesperson, engineer, and applications specialist in the span of about 4 hours. It was impressive. I forgot to mention the autosampler – the best I’ve ever seen."

"I honestly feel proud to use this machine as it is so easy to use but very effective in producing the results."

"It was great to have the chances to try this instrument, it is so accurate and provides great levels of complexity of analysis in such an easy way."

"High resolution immunophenotyping with greater sensitivity and detection rate."

"As someone who has demoed NovoCyte Quanteon Flow Cytometer, I have found that the product is built with the user in mind. The device has a wider (7.2 log) dynamic range for detecting signals compared to competitors. Another pro is that there is direct abs"

"It is very simple to use with the best response and time efficient."