NEBuilder® HiFi DNA Assembly Cloning Kit by New England Biolabs Inc.

Manufacturer New England Biolabs Inc.  |  Available Worldwide
5.0
/
5.0
  |  4 reviews
NEBuilder® HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–80 bp). It has utility for the synthetic biology community, as well as those interested in one-step cloning of multi... Read more


NEBuilder® HiFi DNA Assembly Cloning Kit by New England Biolabs Inc. product image
NEBuilder® HiFi DNA Assembly Cloning Kit

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Average Rating: 5.0
4 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2019

  • Organization: University of Louisville



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product! Can't live without it. Great value for the money; will save you time!
Rating: 5.0

  • Application Area: Genetic construct building

"This is the best system we have used for generating vectors for cloning, gene expression, gene disruption, CRISPR/Cas9, etc. We've struggled with this rate-limiting step in genetic engineering for decades and finally have a reliable and effective technique!"

Review date: 25 Jan 2020 | NEBuilder® HiFi DNA Assembly Cloning Kit
  • Status:

    Advanced Reviewer

  • Member since: 2015

  • Organization: IBPS, UPMC, Paris



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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It works like a miracle for complicated cloning.
Rating: 5.0

  • Application Area:Molecular cloning

"This product is a life saver for the molecular biologist. It works like a miracle for complicated cloning."

Review date: 17 Dec 2018 | NEBuilder® HiFi DNA Assembly Cloning Kit
  • Status:

    Advanced Reviewer

  • Member since: 2015

  • Organization: IBPS, UPMC, Paris



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Fast, efficient & reliable product! I highly recommend it for a molecular biology lab
Rating: 5.0

  • Application Area:Molecular cloning

"This is an outstanding product. This makes my life easier and I am not afraid of carrying out any kind of molecular cloning anymore. It works like magic."

Review date: 27 Nov 2018 | NEBuilder® HiFi DNA Assembly Cloning Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: University of Hawaii



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Great results, wouldn't want to live without it
Rating: 5.0

  • Application Area:DNA Assembly Cloning

"This kit has replaced "regular" cloning for me, at least if I need more than 2 fragments cloned into a backbone. Super-easy to use and takes very little time. In my experience, it works better when I use the upper limit of the suggested amounts for the individual fragments. Also, while it dosen't always work as expected, it literally is a hit or miss. Meaning either all clones are correct or none."

Review date: 01 Nov 2017 | NEBuilder® HiFi DNA Assembly Cloning Kit

NEBuilder® HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–80 bp). It has utility for the synthetic biology community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple master-mix format.

  • Assembly and transformation in just under two hours
  • Flexible sequence design (scar-less cloning)
  • No PCR clean-up step required
  • High transformation efficiencies for inserts up to 20 kb
  • Easily adapted for multiple DNA manipulations, including site-directed mutagenesis
  • Includes competent cells 

Product Overview

NEBuilder® HiFi DNA Assembly Cloning Kit by New England Biolabs Inc. product image

NEBuilder® HiFi DNA Assembly Cloning Kit

Manufacturer New England Biolabs Inc.  |  Available Worldwide

5.0 / 5.0 | 4 reviews