Status:
ReviewerMember since: 2021
Organization: Carnegie Institution for Science
Stop spending weeks on cloning.
Application Area: Cloning
"After learning cloning with standard restriction/ligation, I was introduced to Gibson assembly at the start of my postdoc. Using this, I've been able to clone large genes quite efficiently with up to 5 pieces to assemble. I would definitely recommend it to anyone. NEBuilder also has online tools to design your cloning and determine the amount of each part needed so tip top!"
Status:
ReviewerMember since: 2021
Organization: San Diego Biomedical Research Institute
Must try.
Application Area:Measuring replication timing in different cell lines.
"It is awesome cloning technique. It is very easy, super efficient and quick. I would highly recommend this product."
Status:
ReviewerMember since: 2019
Organization: University of Louisville
Great product! Can't live without it. Great value for the money; will save you time!
Application Area:Genetic construct building
"This is the best system we have used for generating vectors for cloning, gene expression, gene disruption, CRISPR/Cas9, etc. We've struggled with this rate-limiting step in genetic engineering for decades and finally have a reliable and effective technique!"
Status:
Advanced ReviewerMember since: 2015
Organization: IBPS, UPMC, Paris
It works like a miracle for complicated cloning.
Application Area:Molecular cloning
"This product is a life saver for the molecular biologist. It works like a miracle for complicated cloning."
Status:
Advanced ReviewerMember since: 2015
Organization: IBPS, UPMC, Paris
Fast, efficient & reliable product! I highly recommend it for a molecular biology lab
Application Area:Molecular cloning
"This is an outstanding product. This makes my life easier and I am not afraid of carrying out any kind of molecular cloning anymore. It works like magic."
Status:
ReviewerMember since: 2015
Organization: University of Hawaii
Great results, wouldn't want to live without it
Application Area:DNA Assembly Cloning
"This kit has replaced "regular" cloning for me, at least if I need more than 2 fragments cloned into a backbone. Super-easy to use and takes very little time. In my experience, it works better when I use the upper limit of the suggested amounts for the individual fragments. Also, while it dosen't always work as expected, it literally is a hit or miss. Meaning either all clones are correct or none."
NEBuilder® HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–80 bp). It has utility for the synthetic biology community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple master-mix format.
Manufacturer New England Biolabs Inc. | Available Worldwide
5.0 / 5.0 | 6 reviews