Member since: 2018
Organization: Galapagos NV
The nanoBRET assay is set-up for several projects in our company and we recommend it!
Application Area: evaluate compounds for their inhibitory activity
"The nanoBRET assay has the additional advantage of using a ratiometric readout, allowing intra-well normalization and showing increased assay quality. It enables medium/high quality results depending on the constructs used. It makes the link between in vitro and in vivo assays."
The NanoBRET™ Target Engagement (TE) Intracellular BET BRD Assay measures compound binding at select BET bromodomain target proteins within intact cells. This target engagement assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells