Elucidating PROTAC MoA with live-cell kinetic monitoring of ternary complex formation and target protein ubiquitination

18 May 2021

In this application note, BMG LABTECH demonstrates how the CLARIOstar with ACU can be used with NanoBRET™ technology, developed by Promega, to monitor PROTAC function in live cells.

NanoBRET™ Target Engagement BET BRD Assays

Promega Corp.

The NanoBRET™ Target Engagement (TE) Intracellular BET BRD Assay measures compound binding at select BET bromodomain target proteins within intact cells. This target engagement assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells

(1)

Links

Tags