The myTXTL® Linear DNA Kit is particularly useful for the screening of DNA libraries that were generated by PCR amplification. In combination with the ability to utilize an automated liquid handling system for Master Mix dispensing, myTXTL® Linear DNA Expression Kit is ideal for high-throughput applications. The myTXTL® Linear DNA Expression Kit is also recommended to produce live bacteriophages starting from their whole RNA or DNA genome. This can be employed to study phage therapy, a promising approach to treatpathogenic bacterial infections.
The myTXTL® Linear DNA Expression Kit contains an engineered E. coli cell extract, energy buffer and amino acids mix ready-to-use for in vitro protein production in a single tube. In vitro protein synthesis is initialized by adding a linear DNA fragment. A linear fragment of P70a-deGFP plasmid is included in the kit as the positive control.